Full Length Research Paper
Abstract
The aim of our work was the overexpression of the abiotic stress-inducible dehydrin protein, namely RAB16A, from rice in the BL21 strain of Escherichia coli. The Rab16Atranscript of 0.5 Kbp was amplified from the total RNA of the salt-tolerant indica rice cultivar Nonabokra by RT-PCR and cloned into the expression vector pGEX-3X. The 47 kDa protein, expressed as GST: RAB16A fusion protein, after 2 mM IPTG-mediated induction, was collected as S10 fraction and purified through glutathione-sepharose affinity resin. Immunoblot analysis with the maize dehydrin antiserum showed cross-reaction with the above band, but not with GST protein alone, showing functional expression of the heterologous RAB16A protein in the bacterial system.
Key words: Fusion protein, Glutathione-sepharose, GST: RAB16A, pGEX-3X.
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