African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12179

Full Length Research Paper

In vitro micropropagation of grape vine (Vitis vinifera L.) from nodal culture

Beza Kinfe
  • Beza Kinfe
  • Ethiopian Biotechnology Institute, Biotechnology Center, P. O. Box 2490, Addis Ababa, Ethiopia.
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Tileye Feyssa
  • Tileye Feyssa
  • Institute of Biotechnology, College of Natural Science, Addis Ababa University, P. O. Box 1176, Addis Ababa, Ethiopia.
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Girma Bedada
  • Girma Bedada
  • Centre of Plant Biology in Uppsala, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden.
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  •  Received: 23 November 2016
  •  Accepted: 22 June 2017
  •  Published: 25 October 2017

Abstract

The objective of this study was to optimize a protocol for in vitro micropropagation of selected grape vine varieties. Preliminary studies were conducted to optimize the duration of sterilization of the explants. For shoot initiation experiment nodes of the three varieties of grape vines were cultured on MS medium (Murashige-Skoog’s, 1962) supplemented with five different concentrations of 6-Benzylaminopurine (BAP) and the control. Various experiments were carried out to optimize shoot multiplication using MS medium supplemented with different concentrations of BAP alone or in combination with Indole-3-butyric acid (IBA). To optimize root induction, different concentrations of Indole-3-acetic acid (IAA) were used. Sterilization of explants using 1% of NaOCl for 7 min duration was optimum. Chenin blanc showed high percentage of survival rate (96%) followed by Ugni blanc and Canonannon (88%) at 0.5 mg/L BAP. Among the different concentrations and combination of Plant Growth Regulators (PGRs) used for multiplication, maximum mean number of shoots 7.2, 6.7, and 6.1 was achieved at 1 mg/L BAP combined with 0.1 mg/L IBA for Chenin blanc, Canonnanon and Ugni blanc, respectively. All varieties induced root for all the treatments used including the control but good roots were found on MS medium supplemented with 2 and 4 mg/L of IAA. The plantlets were acclimatized in the glasshouse and survival percentage was 92% for Chenin blanc followed by 78.6 and 73.9% for Ugni blanc and Canonannon, respectively. Thus, the achievements of this study will play a big role in the grape vine culture program.

Key words: Vitis vinifera L., acclimatization, explant, cytokinin, auxin.