African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12258

Full Length Research Paper

Biochemical profile of cuttings used for in vitro organogenesis of Prunus africana: An endangered species in Cameroon

Justine Germo Nzweundji*
  • Justine Germo Nzweundji*
  • Medicinal Plants and Traditional Medicine Research Centre, Institute of Medical Research and Medicinal Plants Studies (IMPM). P. O. Box 6163 Yaounde, Cameroon; Department of Biological Science, Higher Teacher Training College, University of Yaounde I. P.O. Box 47 Yaounde, Cameroon.
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Nicolas Niemenak
  • Nicolas Niemenak
  • Department of Biological Science, Higher Teacher Training College, University of Yaounde I. P.O. Box 47 Yaounde, Cameroon.
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Doungous Oumar
  • Doungous Oumar
  • Jay P. J. Biotechnology Laboratory, Institute of Agricultural Research for Development, P.O. Box 25, Buea, Ekona, Cameroon.
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Julie Judith Tsafack
  • Julie Judith Tsafack
  • Medicinal Plants and Traditional Medicine Research Centre, Institute of Medical Research and Medicinal Plants Studies (IMPM). P. O. Box 6163 Yaounde, Cameroon.
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Koffi Konan
  • Koffi Konan
  • IngateyGen LLC, 410 Interpath Parkway Unit J, Elisabeth City North Carolina 27909, USA.
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Leopold Nyochembeng
  • Leopold Nyochembeng
  • Department of Natural Resources and Environmental Sciences Alabama A&M University, P.O. Box 1208, Normal, 35762, Hunstville Alabama, USA.
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Christelle Ngamegne Noumi
  • Christelle Ngamegne Noumi
  • Department of Biological Science, Higher Teacher Training College, University of Yaounde I. P.O. Box 47 Yaounde, Cameroon.
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Nehemie Donfagsiteli Tchinda
  • Nehemie Donfagsiteli Tchinda
  • Medicinal Plants and Traditional Medicine Research Centre, Institute of Medical Research and Medicinal Plants Studies (IMPM). P. O. Box 6163 Yaounde, Cameroon;Department of Biological Science, Higher Teacher Training College, University of Yaounde I. P.O. Box 47 Yaounde, Cameroon.
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Denis Omokolo Ndoumou
  • Denis Omokolo Ndoumou
  • Department of Biological Science, Higher Teacher Training College, University of Yaounde I. P.O. Box 47 Yaounde, Cameroon.
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  •  Received: 21 September 2014
  •  Accepted: 03 August 2015
  •  Published: 19 August 2015

Abstract

Prunus africana (Kanda stick) is a valuable medicinal tree classified as priority species for domestication in Cameroon. To measure the ability for developing axillary buds and multiple shoots in relation with the origin and state of explants, some biochemical parameters were assessed in eight types of single-node cuttings. These cuttings were treated with different concentrations of kinetin (kin) indole butyric acid (IBA) and thidiazuron (TDZ) and cultured in Murashige and Skoog (MS) medium. The glutelin content (1.13 ± 0.201 mg/mg FW) was higher compared to those of total albumin, globulin and prolamin and most representative at mature vegetative apical stage (MVA). The highest (15.60 ± 1.966 mg/mg FW) and lower (2.82 ± 0.072 mg/mg FW) amount of phenol compound was observed in the mature dormant stages and immature vegetative distal stage (IVD), respectively. Regarding the peroxidases, highest activity (0.453 ± 0.055 OD/mg/ FW.5 min-1) was obtained in mature dormant apical stage. Apart from IVA state, there was no reactive explant cultured in vitro. The high percentage of budding and proliferation of budding (100%) and rooting (100%) were achieved with 12 µM Kin and 24 µM IBA, respectively. In the presence of 24 µM TDZ, multiple shoot was induced with a maximum of 5 shoots/explants. Micropropagation success of P. africana is influenced by cytokinin, physiological and biochemical status of single node cutting.

 

Key words: Biochemical profile, Prunus africana, budding, multiple shoots, rooting.

Abbreviation

MS, Murashige and Skoog; Kin, kinetin; IBA, indole butyric acid; MVA, mature vegetative apical; IVD, immature vegetative distal; TDZ , Thidiazuron.