African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12227

Full Length Research Paper

Inhibition of carbon disulfide on bio-desulfurization in the process of gases purification

Ziyu Song
  • Ziyu Song
  • National key Laboratory of Biochemical Engineering, Institute of process Engineering, Chinese Academy of Sciences, Beijing 100190, People’s Republic of China; Graduate University of Chinese Academy of Sciences, Beijing 100049, People’s Republic of China.
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Qiang Li
  • Qiang Li
  • National key Laboratory of Biochemical Engineering, Institute of process Engineering, Chinese Academy of Sciences, Beijing 100190, People’s Republic of China.
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Dan Wang
  • Dan Wang
  • College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044, People’s Republic of China.
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Hang Tang
  • Hang Tang
  • Graduate University of Chinese Academy of Sciences, Beijing 100049, People’s Republic of China; Graduate University of Chinese Academy of Sciences, Beijing 100049, People’s Republic of China.
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Maohua Yang
  • Maohua Yang
  • National key Laboratory of Biochemical Engineering, Institute of process Engineering, Chinese Academy of Sciences, Beijing 100190, People’s Republic of China.
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Jianmin Xing*
  • Jianmin Xing*
  • National key Laboratory of Biochemical Engineering, Institute of process Engineering, Chinese Academy of Sciences, Beijing 100190, People’s Republic of China.
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  •  Received: 05 April 2012
  •  Published: 16 April 2014

Abstract

Biological desulfurization is a novel technology for the removal of hydrogen sulfide from some biogas or sour gas, in which there are always a certain amounts of carbon disulfide together with much hydrogen sulfide. Nowadays, carbon disulfide is found to have negative effect on the biological desulfurization, but seldom research is afforded to investigate how carbon disulfide inhibits the process of biological desulfurization. In this paper, we investigated the effect of carbon disulfide both on the growth of Thiobacillus thioparus and the resting cells under various concentrations, including 0.01, 0.05, 0.10, 0.15 and 0.20%. In this process, the rate of the cell growth was characterized by the rate of nitrogen consumption in order to solve the problem that the adsorption of cells to sulfur granules have on the accuracy of biomass test. Under the cell density of 23.92 mg N/L, which is lower than the maximum of cell density 36.13 mg N/L, the average rate of thiosulfate oxidation reached the maximum (26.50 S2O32- mg/L-h). Carbon disulfide at titers of 0.01% significantly inhibited the growth of cells, but hardly affected the biological desulfurization of resting cells. Although carbon disulfide at titers of 0.05% had negative effect on the biological desulfurization of resting cells, the effect of inhibition could be relieved by the increased density of resting cells. For the resting cells, the parameters of Michaelis-Menten equation were calculated by the method of Lineweaver-Burk. The Vmax of biological desulfurization was decreased from 27.93 to 14.0 S2O32- mg/L-h, and the Km was increased from 0.264 to 0.884 mM, with the concentration of carbon disulfide rising up from 0.0 to 0.1%. These results show that the growth of cells was sensitive to carbon disulfide, and the resting cells had resistance to the low level of carbon disulfide (0.05%). Thus, the inhibition of carbon disulfide to biological desulfurization should be attributed to T. thioparus growth suppression function.

 

Key words: Carbon disulfide, bio-desulfurization, inhibition, Thiobacillus thioparus, resting cell, gases purification.