African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12278

Full Length Research Paper

Hepatoprotective activity of polyherbal formulation against carbon tetrachloride-induced hepatotoxicity in rats

  K. Gurusamy1*, R. Kokilavani2  and K. Arumuasamy3  
  1Department of Biochemistry,Dr.N.G.P Arts and Science College, Kalappatti Road,Coimbatore- 641 048. Tamilnadu. India. 2Department of Biochemistry,Kongunadu Arts and Science College, Coimbatore – 641 029. India. 3Department of Botany, Kongunadu Arts and Science College, Coimbatore – 641 029. India.
Email: [email protected]

  •  Accepted: 18 June 2010
  •  Published: 06 December 2010



The ethanloic extracts of the polyherbal medicinal plants (Asteracantha longifolia, Cyperus rotundus and Bryophyllum pinnatum) were evaluated for hepatoprotective activity in carbon tetrachloride induced liver damage in rats. The ethanolic extract of polyherbal formulation at 250 mg/kg b.w. exhibited a significant protective effect by lowering serum and liver activities of aspartate transaminase (AST), alanine transaminase (ALT), acid phosphatase (ACP), alkaline phosphatase (ALP), lactate dehydrogenase(LDH), serum bilirubin, serum cholesterol and serum total protein when compared with standard silymarin. The hepatoprotective activity of the extracts may be attributed to increased regeneration of hepatocytes and inhibitory effects on microsomal enzymes.


Key words: Polyherbal formulation, carbon tetrachloride, silymarin.


Abbreviations: AST, Aspartate transaminase; ALT, alanine transaminase; ACP,acid phosphatise; ALP, alkaline phosphatise; LDH, lactate dehydrogenase; PHF,polyherbal formulation.