African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12302

Full Length Research Paper

Influence of vermicompost humic acid on chlorophyll content and acclimatization in banana clone, Enano Guantanamero

Marcia Beatriz Moya Fernandez
  • Marcia Beatriz Moya Fernandez
  • Universidad Agraria de la Habana (UNAH) “Fructuoso Rodríguez Pérez”, Carretera Tapaste y Autopista Nacional, San José de las Lajas, Mayabeque, Cuba.
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Esteban Sanchez Chavez
  • Esteban Sanchez Chavez
  • Centro de Investigación en Alimentos y Desarrollo (CIAD), Unidad Delicias, Av. 4ª. Sur 3820, Fracc. Vencedores del Desierto, Cd. Delicias, Chihuahua. México.
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Daniel Cabezas Montero
  • Daniel Cabezas Montero
  • Universidad Agraria de la Habana (UNAH) “Fructuoso Rodríguez Pérez”, Carretera Tapaste y Autopista Nacional, San José de las Lajas, Mayabeque, Cuba.
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Andres Calderin Garcia
  • Andres Calderin Garcia
  • Federal Rural University of Rio de Janeiro, Soil Science Dept. Rodovia BR 465 km 7, Seropédica, RJ, Brazil.
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Dany Marrero Lopez
  • Dany Marrero Lopez
  • Havana Biofabric, Autopista Nacional Km 23 ½, San José de Las Lajas, Mayabeque, Cuba.
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Eduardo F. Hector Ardisana
  • Eduardo F. Hector Ardisana
  • Escuela Superior Politécnica de Chimborazo, Vicerrectorado de Investigación y Posgrado. Panamerican South Km 1 ½, Riobamba, Ecuador, C.P. ECO60155.
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Sandra Perez Álvarez*
  • Sandra Perez Álvarez*
  • Instituto Politécnico Nacional, CIIDIR-IPN, Unidad Sinaloa, Departamento de Biotecnología Agrícola, Blvd. Juan de Dios Bátiz Paredes No 250. Guasave, Sinaloa, México, C.P. 81101
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  •  Received: 19 September 2016
  •  Accepted: 11 November 2016
  •  Published: 23 November 2016

Abstract

Vermicompost humic acids (VHA) promote plants’ growth because they have similar effects with auxins. The aim of this research was to evaluate the effect of VHA in some physiological indicators in the micropropagation and acclimatization phase of banana clone Enano Guantanamero. Six concentrations were used (0, 10, 20, 30, 40 and 50 mg L-1) to evaluate in vitro the number of leaves, total chlorophyll content and chlorophyll a-b; also in the acclimatization phase, the plant height, stem diameter and number of leaves at three different times (transplant, 25 days and at the end of this phase-50 days) were determined. Root length and roots dry weight were evaluated at the end of the acclimatization. VHA applied improved total chlorophyll, chlorophyll a-b at concentrations of 20 and 50 mg L-1, but not the number of leaves. In the acclimatization stage, plants height at the end of this period (50 days) were higher with 10 mg L-1 (T1)  VHA, the number of leaves increased at 40 mg L-1 (T4) and the stem diameter was higher at both concentrations. In the evaluation of roots length, there were no significant differences, but the number of leaves was higher at 10 and 40 mg L-1 (T1 and T4) and it decreased at 50 mg L-1 (T5); roots dry weight increased at 40 mg L-1 (T4).  VHA promoted chlorophyll content under in vitro conditions, it reduced the period of acclimatization of banana clone Enano Guantanamero, led to a better growth of plants, and it saved time and resources.

Key words: Acclimatization, humic acid, in vitro crop, Musa sp. 

Abbreviation

DRIFT, Diffuse reflectance Fourier-transformed infrared; HS, humic substances; MS, Murashige and Skoog; UV- VIS, ultraviolet-visible spectroscopy; VHA,  vermicompost humic acids.