African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Short Communication

Free radical scavenging activity and phenolic content of Cassia sophera L

Atiqur Rahman1, 2 *, M. Mizanur Rahman3, 4, Md. Mominul Islam Sheik3, M. Mashiar Rahman3, Shabah Mohammad Shadli3 and M. F. Alam5
  1Department of Biotechnology, Daegu University, Kyoungsan 712-714, Korea. 2Department of Applied Chemistry and Chemical Technology, Islamic University, Kushtia 7003, Bangladesh. 3Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia 7003, Bangladesh. 4Dipartimento di Biotecnologie Agrarie, Viale dell’ Università 16, 35020 - Legnaro (Padova), Italy. 5Department of Botany, Rajshahi University, Rajshahi, Bangladesh.
Email: [email protected]

  •  Accepted: 31 March 2008
  •  Published: 16 May 2008

Abstract

 

Dried methanol extract of leaves of Cassia sophera L. was dissolved in distilled water, and then fractioned by re-extracting with n-hexane, chloroform, and ethyl acetate, subsequently. The free radical scavenging activity (FRSA) of methanol extract and various fractions of methanol extract was determined by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The FRSA of ethyl acetate fraction was superior to all other fractions (IC50 = 15.42 μg/ml), which was higher than synthetic antioxidant butylated hydroxyanisole, BHA (18.25 μg/ml). The amount of total phenolic compounds was further determined. The total phenolic content in EtOAc fraction (13.25%) was the highest as compared to other extracts. The results of this study suggest that this plant could serve as a source of natural antioxidants and preservative agents with potential applications in food industries.

 

Key words: Cassia sophera L., radical scavenging activity, phenolic content.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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