Abstract

In vitro shoot regeneration of Mentha piperita L. was investigated using shoot meristems, node, internode and petiole explants. Shoot regeneration was achieved on ½ strength MS salts and vitamins supplemented with various concentrations of BAP, Kin alone or with NAA. Shoot meristems and nodal segments showed variation in the frequency of shoot regeneration with single to multiple shoots. However, internode and petiole explants were not potent and failed to regenerate shoots at some of the tested plant growth regulators concentrations. Variable frequency of rooting was recorded at four different concentrations of auxins. The rooted plants were successfully acclimatized to in vivo conditions in the greenhouse.

Key words: Mentha piperita, plant hormone, organogenesis, shooting.

Abbreviation

Abbreviations: MS, Murashige and Skoog; BAP, N6-benzylaminopurine; Kin, kinetin; IAA, indole acetic acid; IBA, indole butyric acid; NAA, naphthaleneacetic acid.