Abstract

A method to induce indirect regeneration from Hypericum triquetrifolium Turra was described in the present study. Callus was induced from cotyledon explants of 35 days old aseptic seedlings on semi-solid MS supplemented with IAA (0.5 mg.L^-1) combined with BAP (2 mg.L^-1). Meristemoids developed on the surface of callus by decreasing of the cytokinins and plantlet regeneration with 100% frequency through these embryogenic calli occured in semi-solid medium when the PGRs were removed completely. Embryogenic calli obtained during the experiments were analyzed for their hypericin content. And they were found to produce hypericin as 48 µg/g DM. Regenerated plantlets were rooted in MS containing 1 mg/L IAA. The highest percentages (94%) of survival of transferred plantlets to free-living circumstances were limited when they were acclimatized in sand : peat : perlite (1:1:1; v/v/v) mixture.

Key words: Hypericum triquetrifolium, indirect regeneration, hormone-free MS

Abbreviation

IAA, Indole-3-acetic acid; BAP, 6-benzylaminopurine; NAA, naphtelene aceticacid; BA, benzyl adenine; MS, Murashige-Skoog; PGR, plant growth regulator; DM, dry mass; 2,4-D, 2,4-dichloro-phenoxyacetic acid.