African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12209

Full Length Research Paper

Strain improvement and optimization of the media composition of chitosanase-producing fungus Aspergillus sp. CJ 22-326

Xiao-E Chen1*, Xu-Bo Fang1 and Wen-Sui Xia2
1School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316004, P.R. China. 2School of Food Science, Southern Yangtze University, Wuxi 214036, P.R. China.
Email: [email protected]

  •  Accepted: 18 June 2008
  •  Published: 18 July 2008

Abstract

 

A mutant Aspergillus sp. CJ 22-326-14 with higher production of chitosanase was selected by serial mutation procedure, and the optimization of medium components was performed using one-factor-at-a-time combined with orthogonal array design method. The results showed that chitosan, wheat bran and (NH4)2SO4 were the most important components by one-factor-at-a-time experiments. And the optimal concentrations were obtained by orthogonal array design method as (g/L): chitosan 20, wheat bran 10, (NH4) 2SO4 2, KH2PO4  2 and MgSO4·7H2O  0.5. After the strain improvement and optimization of the media, the chitosanase activity from Aspergillus sp. CJ 22-326-14 reached to 3.92 U/mL in 250 mL flasks, which was nearly 4-fold increase of chitosanase production at the parent lowest level.

 

Key words: Aspergillus sp., chitosanase, mutant, fermentation, optimization, orthogonal array design method.