African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12254

Full Length Research Paper

The use of Amazon fungus (Trametes sp.) in the production of cellulase and xylanase

Salony Aquino Pereira
  • Salony Aquino Pereira
  • Laboratory of Applied Chemistry and Technology, Chemical Engineering Course, School of Technology, State University of Amazonas, CEP 69050-020, Manaus-AM, Brazil.
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Rafael Lopes e Oliveira
  • Rafael Lopes e Oliveira
  • Laboratory of Applied Chemistry and Technology, Chemical Engineering Course, School of Technology, State University of Amazonas, CEP 69050-020, Manaus-AM, Brazil.
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Sergio Duvoisin Jr.
  • Sergio Duvoisin Jr.
  • Laboratory of Applied Chemistry and Technology, Chemical Engineering Course, School of Technology, State University of Amazonas, CEP 69050-020, Manaus-AM, Brazil.
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Leonor Alves de Oliveira da Silva
  • Leonor Alves de Oliveira da Silva
  • Laboratory of Fermentative Processes, Department of Antibiotics, Biological Sciences Center, Federal University of Pernambuco, Brazil, CEP 50670-901, Recife-PE, Brazil.
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Patrícia Melchionna Albuquerque
  • Patrícia Melchionna Albuquerque
  • Laboratory of Applied Chemistry and Technology, Chemical Engineering Course, School of Technology, State University of Amazonas, CEP 69050-020, Manaus-AM, Brazil.
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  •  Received: 06 April 2016
  •  Accepted: 20 April 2016
  •  Published: 18 May 2016

Abstract

In order to use the residue from the beneficiation of Brazil nuts (Bertholletia excelsa HBK) as substrate in solid-sate fermentation (SSF), in this work, the production of cellulase and xylanase by the fungus Trametes sp. was investigated, using the residue as a carbon source. Employing a 24-1 fractional experimental design, the influence of substrate moisture, nutrient addition and inoculum quantity on enzymatic activities was verified. Moisture was detected to be statistically significant for the production of both enzymes, and increasing the moisture leads to the improvement of cellulase and xylanase activities. Nitrogen and phosphate were also important for enzymes production by the Amazon Trametes sp. The use of this Amazon strain to obtain cellulase and xylanase via SSF of Brazil nut residue appears to be feasible when maintaining substrate moisture at 80%, nitrogen source at 0.9% and low inoculum concentrations.

 

Key words: Cellulolytic activity, xylanolytic activity, Trametes sp., experimental design.