Abstract

The principal objective of this study was to assess the presence of Salmonella sp. in imported amphibians and reptiles. A total of 49 samples were collected from commercial pet shops and life science museums in Korea and examined via the traditional detection method (TDM) and the PCR method. Salmonella typhimuriumand Salmonella enteritidis were screened via multiplex PCR (mPCR). The results ofthe TDM showed a positive isolation in 18.4% of the samples, whereas the PCR method had a 28.6% positive detection rate. These results indicate the possibility that humans can contact Salmonella infections from amphibians and reptiles. Our findings are also important from a public health standpoint. Additionally, the presence ofSalmonella sp. in these samples indicates the necessity of appropriate quarantine practices to be established.

Key words: Amphibian, PCR, reptile, Salmonella sp.

Abbreviation

TDM, Traditional detection methods; mPCR, multiplex polymerase chain reaction; BPW, buffered peptone water; RV, Rappaport Vassiliadis; XLD, xylose lysine desoxycholate agar; BGA, brillant green agar; TSB, tryptic soy broth.