African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12474

Full Length Research Paper

Comparative studies on inducers in the production of naringinase from Aspergillus niger MTCC 1344

V. Vinoth Kumar
Department of Biotechnology, Kumaraguru College of Technology, Coimbatore, Tamil Nadu, India
Email: [email protected]

  •  Accepted: 11 February 2010
  •  Published: 08 November 2010

Abstract

 

This research provides detailed systematic study of the effect of different inducers (hesperidin, naringenin, naringin, rhamnose and rutin) in naringinase production by Aspergillus niger MTCC 1344. Cultures were carried out in shake flasks and they produce extracellular naringinase in a complex (molasses, peptone and salts) medium. The optimized concentration (%) of naringin, rhamnose, naringenin, rutin and hesperidin for maximized naringinase production are 0.1, 0.375, 0.01, 0.2 and 0.2, respectively. Compared with control, inducers increased the naringinase production by many folds in the order of naringin (6.63) > rhamnose (4.87) > naringenin (3.26) > rutin (2.84) > hesperidin (2.35). Under optimum conditions, about 9.68 units of enzyme per ml complex medium containing naringin were obtained on the 7th day. The activity to inducer (A/I) ratio was 968 Ug-1 naringin, and the cultivation time was shorter in submerged production. The results indicate that naringinase activity used naringin as an inducer which was significantly higher than the other four inducers. Therefore naringin is recommended for naringinase production.

 

Key words: Naringin, naringenin, rutin, hesperidin, rhamnose, naringinase,Aspergillus, inducer, molasses.