Abstract

Bulblets propagation by tissue culture was one of the key techniques in the production of lily (Lilium) bulbs. Therefore, in vitro micro-propagation of lily bulblets was studied in detail in this paper. L A hybrids lily cultivar ‘eyeliner’ was selected as the materials. By using the method of orthogonal design, the following were concluded from the research: the optimum treatment and disinfection methods of ‘eyeliner’ bulb scales was soaking in 1:500 carbendazim solution for 30 min, disinfection in 75% alcohol for 10 to 60 s, disinfection in 2% NaClO solution for 15 min; the optimum medium for bud induction of ‘eyeliner’ was MS + 0.5 mg·L^-1 6-benzyl aminopurine (6-BA) + 0.1 mg·L^-1naphlene acetic acid (NAA) + 90 g·L^-1sucrose, and 25°C and in darkness; the optimum medium for bulblets induction of ‘eyeliner’ was 2MS + 1.0 mg·L^-1 6-BA + 0.5 mg·L^-1 NAA + sucrose 90 g·L^-1 + Paclobutrazol (PP333) 2 mg·L^-1; the optimum culture condition for bulblets induction of ‘eyeliner’ was 20°C, 14 h·day^-1 lightness + 10 h·day^-1 darkness. The optimum medium for rooting culture of ‘eyeliner’ was ½ MS + 0.8 mg·L^-1 NAA + 3 g·L^-1 activated charcoal, 20°C, 14 h·day^-1 lightness + 10 h·day^-1 darkness.

Key words: Lily bulb, orthogonal experiment, in vitro micro-propagation.