African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12213

Full Length Research Paper

Optimization of Saccharomyces cerevisiae SKM10 single cell protein production from mango (Magnifera indica L.) waste using response surface methodology

Marius K. SOMDA
  • Marius K. SOMDA
  • Laboratory of Microbiology and Microbial Biotechnology, Research Center in Biological Food and Nutrition Sciences (CRSBAN), Department of Biochemistry and Microbiology, University Ouaga1 Pr Joseph KI-ZERBO, Burkina Faso.
  • Google Scholar
Cheik Amadou Tidiane OUATTARA
  • Cheik Amadou Tidiane OUATTARA
  • Laboratory of Food Technology, Department of Biochemistry and Microbiology, University Ouaga1 Pr Joseph KI-ZERBO, Burkina Faso.
  • Google Scholar
Iliassou MOGMENGA
  • Iliassou MOGMENGA
  • Laboratory of Microbiology and Microbial Biotechnology, Research Center in Biological Food and Nutrition Sciences (CRSBAN), Department of Biochemistry and Microbiology, University Ouaga1 Pr Joseph KI-ZERBO, Burkina Faso.
  • Google Scholar
Mahamadi NIKIEMA
  • Mahamadi NIKIEMA
  • Laboratory of Microbiology and Microbial Biotechnology, Research Center in Biological Food and Nutrition Sciences (CRSBAN), Department of Biochemistry and Microbiology, University Ouaga1 Pr Joseph KI-ZERBO, Burkina Faso.
  • Google Scholar
Ibrahim KEITA
  • Ibrahim KEITA
  • Laboratory of Microbiology and Microbial Biotechnology, Research Center in Biological Food and Nutrition Sciences (CRSBAN), Department of Biochemistry and Microbiology, University Ouaga1 Pr Joseph KI-ZERBO, Burkina Faso.
  • Google Scholar
Nicolas OUEDRAOGO
  • Nicolas OUEDRAOGO
  • Laboratory of Microbiology and Microbial Biotechnology, Research Center in Biological Food and Nutrition Sciences (CRSBAN), Department of Biochemistry and Microbiology, University Ouaga1 Pr Joseph KI-ZERBO, Burkina Faso.
  • Google Scholar
Desire TRAORE
  • Desire TRAORE
  • Laboratory of Microbiology and Microbial Biotechnology, Research Center in Biological Food and Nutrition Sciences (CRSBAN), Department of Biochemistry and Microbiology, University Ouaga1 Pr Joseph KI-ZERBO, Burkina Faso.
  • Google Scholar
Alfred S.TRAORE
  • Alfred S.TRAORE
  • Laboratory of Food Technology, Department of Biochemistry and Microbiology, University Ouaga1 Pr Joseph KI-ZERBO, Burkina Faso.
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  •  Received: 17 August 2017
  •  Accepted: 15 September 2017
  •  Published: 08 November 2017

Abstract

The present study aims at optimizing the production of Saccharomyces cerevisiae SKM10 single cell proteins (SCP) from mango waste. To optimize the production process, the effect of two independent variables was evaluated by a three-level full factorial design using response surface methodology (RSM). The parameters studied were inoculum size (2 to 12% v/v) and concentration of substrates (5 to 10 g/L). Analytical methods were used to determine yeast cell biomass, sugar and protein content in yeast. Under optimal conditions, sugar content and maximum cell dry weight attained were 15.28 and 29.85% (w/w). Optimal protein content of 79.14% (w/w) was achieved at 8% (v/v) inoculum and 8% (g/L) substrate concentration. These results have provided useful information on how to improve the production by RSM and suggested that S. cerevisiae SKM10 might be applied effectively to produce SCP using mango waste as a low-cost substrate.

 

Key words: Mango waste, Saccharomyces cerevisiae, bioconversion, production, single cell protein.