Abstract

The present paper deals with the effect of light intensity, photoperiod and growth room temperature on in-vitro morphogenetic responses of leaf sheath explants of sugarcane varieties, CoS 96258 and CoS 99259. High frequency callus initiation was recorded in leaf sheath explants incubated in dark for 10 to 15 days and then, transferred in light. Maximum shoot regeneration and number of shoots per culture could be recorded under 16 h photoperiod of 4000 lux light intensity at a growth room temperature of 25 ± 2°C in both varieties of sugarcane.

Key words: Callus culture, shoot regeneration, in-vitro growth condition, sugarcane.