African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12294

Full Length Research Paper

Immobilization of the Carioca bean (Phaseolus vulgaris L.) extract on SiO2-TiO2 aiming the determination of the herbicide Simazine

Francisca das Chagas Sobral Silva Mihos
  • Francisca das Chagas Sobral Silva Mihos
  • Department of Biochemical Engineering, School of Chemistry, Technology Center, Federal University of Rio de Janeiro, 21941-909, University City, Rio de Janeiro - RJ, Brazil.
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Emerson Schwingel Ribeiro
  • Emerson Schwingel Ribeiro
  • Institute of Chemistry, Federal University of Rio de Janeiro, av. Athos da Silveira Ramos, 149, Block A, 7th Floor, CEP 21941-909, University City, Rio de Janeiro-RJ, Brazil.
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Maria Helena Wohlers Morelli Cardoso
  • Maria Helena Wohlers Morelli Cardoso
  • INCQS – National Institute of Health Quality Control, Fio Cruz,Rio de Janeiro-RJ, Brazil.
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Lucia Helena Pinto Bastos
  • Lucia Helena Pinto Bastos
  • INCQS – National Institute of Health Quality Control, Fio Cruz,Rio de Janeiro-RJ, Brazil.
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Lívia Maria da Costa Silva
  • Lívia Maria da Costa Silva
  • Department of Agricultural and Environmental Engineering, School of Engineering, Federal Fluminense University, 24210-240 Niterói - RJ, Brazil.
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Andréa Medeiros Salgado
  • Andréa Medeiros Salgado
  • Department of Biochemical Engineering, School of Chemistry, Technology Center, Federal University of Rio de Janeiro, 21941-909, University City, Rio de Janeiro - RJ, Brazil.
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  •  Received: 16 October 2019
  •  Accepted: 07 February 2020
  •  Published: 29 February 2020

Abstract

Pesticides are identified as hazardous contaminants due to high toxicity and persistence in the environment. In this context, it is necessary to monitor these pollutants in the environment in situ, with the use of low cost analytical techniques and of quick response. Biosensors are presented as a complementary analytical tool to more complex techniques, such as chromatography. The present work aims to develop, in a simple and low cost way, an amperometric biosensor for the detection of simazine in aqueous samples from the inhibition of the enzyme peroxidase extracted from the pinto bean (Phaseolus vulgaris L.). For the development of the biosensor, the enzyme peroxidase was extracted in sodium phosphate buffer solution, of pH 6.0, and pre-purified by ammonium sulfate precipitation 40% (w / v) salt saturation to 50.0 ml of the protein extract. Peroxidase was characterized by electrophoresis and immobilized on silica-titanium oxide, synthesized by the sol-gel method in the proportions of 70% / 30% respectively. The biosensor showed linearity in the concentration range between 1.0 and 6.0 μgL-1 of simazine (R2 = 0.989) in the potential range 0.20 V to 0.30 V vs Ag / AgCl by the square wave voltammetry technique. The curve was obtained in the presence of 2.0 mgL-1 phenol and 3% H2O2 (v / v)), where the current density signal decreased in the presence of successive additions of the simazine inhibitor. The biosensor was applied to a standard simazine-fortified sample and detected the presence of simazine at the concentration of 35.05 μgL-1. Even showing a relative percentage error of 12.63%, when compared with the result of the analysis by high performance liquid chromatography, the biosensor is useful to monitor the presence or absence of the simazine pesticide in the solution.

 

Key words: Simazine, Biosensor, Carioca beans (Phaseolus vulgaris L.).