Ruminant infection models for Mycoplasma mycoides are invasive and laborious factors that may interfere with the experiment outcomes. This brief review presents an alternative method that may minimize on the mentioned challenges earlier mentioned. M. mycoides subsp. capri GM12 and M. mycoides subsp. capri YCP1.1 ∆68 strains were used; the former, causing a septicemia in goats. An intra-tracheal inoculation technique was employed after aseptic preparation as an alternative to the common intubation method. The former is cheaper, less laborious to handlers, with minimal stress to goats and more time-efficient. In conclusion, this may be used as an alternative to the intubation method. The described technique here will be useful in contagious bovine pleuropneumonia and contagious caprine pleuropneumonia research with modifications, diseases for cattle and small ruminants, respectively.
Key words: Mycoplasma mycoides, animal infection model, ruminants.