African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12227

Table of Content: December 2019; 18(32)

December 2019

Effect of ethanolic leaf extract of Vinca major L. on biochemical parameters and glucose level of alloxan induced diabetic rats

In view of World Health Organization (WHO) recommendation on the use of medicinal plants for the management of diabetes mellitus, this study evaluated the effect of ethanolic leaf extract of Vinca major L. on biochemical parameters and glucose level of alloxan induced diabetic rats as objective. Sixty-four male Wistar albino rats were induced with diabetes by a single intraperitoneal injection and were separated into...

Author(s): Monago-Ighorodje Comfort, Duru Majesty, Adindu Eze, Nwauche Kelechi, Ezekwe Ahamefula, Nosiri Ijeoma, Odika Prince, Onyeabo Chimaraoke, Ogar Ishade, Berezi Ebitimi Peter, Ugoh Austin Ikenna, Eboagwu Ijeoma and Otta Emmanuel  

December 2019

Development of a rice yellow mottle virus amplicon biotechnological tool for the production of high value proteins in rice

Lately, advances on biotechnology have led to the emergence of plants as bioreactor for the production of molecules of industrial, pharmaceutical and agribusiness interests. One of the major advances was the exploitation of plant viruses as “amplifier” for the protein production. This amplicon technology relies on viral vector engineered to contain a gene encoding candidate protein that is produced in...

Author(s): Pingdwende Kader Aziz Bamogo,, Christophe Brugidou, Fidèle Tiendrébéogo, Drissa Sérémé, Florencia Wendkuuni Djigma, Jacques Simpore and Séverine Lacombe,

December 2019

An alternative caprine infection model for Mycoplasma mycoides

Ruminant infection models for Mycoplasma mycoides are invasive and laborious factors that may interfere with the experiment outcomes. This brief review presents an alternative method that may minimize on the mentioned challenges earlier mentioned. M. mycoides subsp. capri GM12 and M. mycoides subsp. capri YCP1.1 ∆68 strains were used; the former, causing a septicemia in goats. An intra-tracheal inoculation technique was...

Author(s): Ssajjakambwe Paul, Elise Schieck, Anne Liljander, Tweyongyere Robert, Matovu Enock, Kazibwe Ann, Nanteza Ann and Mwiine Frank

December 2019

Phenotypic and molecular characterization of extended spectrum β-lactamase producing Pseudomonas aeruginosa in Nigeria

This present study was undertaken for detection of extended spectrum β-lactamases (ESBLS) enzyme genes among clinical isolates of Pseudomonas aeruginosa using phenotypic and molecular techniques. Thirty-four P. aeruginosa isolates from different hospitals in Nsukka and University of Nigeria Teaching Hospital (UNTH), Enugu were screened for the presence of ESBL-encoding genes. Phenotypic screening for ESBL producers...

Author(s): Martina C. Agbo, Ifeoma M. Ezeonu, Maurice N. Odo, Chukwuebuka  M. Ononugbo, Beatrice O. Onodagu, Chinelo C. Eze, Ezinwanne  N. Ezeibe and Chizoba A. Ozioko  

December 2019

Evaluation of Lactobacillus spp. isolated from locally consumed probiotic food in Nsukka, Enugu State, Nigeria for antimicrobial activity utilizing agar well diffusion and pH tolerance tests

Antimicrobial activity against entero-pathogens and tolerance to acid stress are crucial characters of probiotic bacteria. Lactobacillus spp. isolates were phenotypically characterized using colony observation, catalase test and Gram stain reaction.  The pH (1.5, 2.5 and 3.5) tolerance of each isolate was evaluated at 0 and 4 h. The antibacterial activities of the isolates were tested against pathogenic strains of...

Author(s): Dinebari P. Berebon, Kenneth C. Ofokansi, Anthony A. Attama, Christopher O. Eze, Restus C. Onwusoba, Chukwudi T. Ugwu, Charity C. Eze and Somtochukwu A. Evurani  

December 2019

First report of bovine papillomavirus genotypes in cutaneous lesions by polymerase chain reaction (PCR) and direct sequencing in Panama

Bovine papillomavirus (BPV) causes benign tumours in the mucosal and cutaneous epithelium and is characterized by the presence of warts. The present study includes the molecular identification of BPV strains in samples of warts using degenerate polymerase chain reaction (PCR) primers FAP59/64. Skin biopsies were taken from crossbred cattle from two experimental farms of Instituto de Investigación Agropecuaria de...

Author(s): Rita González, Manuel Murillo, Hilda Elena Castillo M., Marcelino Jaén  and Axel Villalobos-Cortés

December 2019

Survey of the incidence and distribution of groundnut rosette disease in major groundnut-producing regions of Western Kenya

Groundnut rosette disease (GRD) is the most important viral disease of groundnuts in sub-Saharan Africa. In Kenya, GRD infection especially before flowering results in 100% loss in pod yield. Surveys were conducted in 2016 and 2017 to determine the incidence and distribution of GRD in five major groundnut growing Counties of western Kenya. A structured questionnaire was used to assess GRD incidence and severity and...

Author(s): Eric O. Were, Evans N. Nyaboga and Elijah M. Ateka  

December 2019

In vitro regeneration of coconut (Cocos nucifera L) through indirect somatic embryogenesis in Kenya

Regeneration of the coconut (Cocos nucifera L) through indirect somatic embryogenesis using 9 to 12 months embryos explants was established in Y3 medium supplemented with 100 to 250 µM 2,4 -dichlorophenoxyacetic acid (2, 4-D) alone and in combination with gibberellic acid (GA3), 6-benzylaminopurine (BAP) and thiadiazuron (TDZ). The highest callus induction (100%) was observed in medium containing 150 and 250...

Author(s): Caren C. Bett, Cecilia M. Mweu and Aggrey B. Nyende