African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12227

Full Length Research Paper

Potential cyclodextrin glycosyltransferase producer from locally isolated bacteria

Norhayati Ramli1, Suraini Abd-Aziz1*, Mohd Ali Hassan1, Noorjahan Alitheen2and Kamarulzaman Kamaruddin3
1Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti of Putra Malaysia, 43400 Serdang, Selangor, Malaysia. 2Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences,  Universiti of Putra Malaysia, 43400 Serdang, Selangor, Malaysia. 3Environment and Bioprocess Technology Centre, SIRIM Berhad, No. 1, Persiaran Dato’ Menteri, Section 2, 40911 Shah Alam, Malaysia.
Email: [email protected]

  •  Accepted: 17 August 2010
  •  Published: 25 October 2010

Abstract

Cyclodextrin glycosyltransferase (CGTase) is one of the most important groups of microbial amylolytic enzymes that have been used for degradation of starch to yield cyclodextrin (CD) via cyclization reaction. The increasing demand for CD in industrial application has led to an extensive study about CGTase which begin with screening, isolation and characterization of CGTase-producing bacteria. The identification of CGTase producer involves the use of solid media containing phenolphthalein and methyl orange as indicators that was detected by the colour changes. The formations of clearance zone around the bacterial colony in the starch-containing medium were observed and the diameters were measured to gauge the hydrolytic efficiency of the bacteria. Out of 65 soil bacterial samples screened, Bacillus sp. NR5 UPM was identified as the most prolific CGTase producer, which produced highest CGTase activity (11.709 U/ml) and highest b-CD concentration (2.504 mg/ml) with a-CD:b-CD:g-CD ratio was 0.5:91.1:8.4 when using raw soluble starch as a substrate. It also showed as the best CGTase producer when using sago starch as a substrate (15.514 U/ml). This isolate was known as a raw starch-degrading enzyme producer since it has the capability touse raw starch as a substrate. Thus, in the future, this new isolate perhaps can share the biggest market in industrial application.

 

Key words: Cyclodextrin, cyclodextrin glycosyltransferase, starch.

Abbreviation

CGTase, Cyclodextrin glycosyltransferase; CD, cyclodextrin;HPLC, high performance liquid chromatography.