African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12213

Table of Content: 25 October 2010; 9(43)

October 2010

Polymorphism in TNP-1 gene of Murrah buffalo bulls

Transition nuclear proteins (TPs), the major proteins found in chromatin of condensing spermatids, have been reported to be important for histone displacement and chromatin condensation during mammalian spermatogenesis. In the present study, transition nuclear protein-1 (TNP-1) gene was analyzed using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) technique- to detect polymorphism in...

Author(s): S. K. Panigrahi and B. R. Yadav

October 2010

Analysis of glutathione S-transferase (M1, T1 and P1) gene polymorphisms in Iranian prostate cancer subjects

Glutathione S-transferase enzymes are active in detoxifying a wide number of endogenous and exogenous chemical carcinogens and subsequently, are crucial in protecting the DNA. Several studies show some differences in association of glutathione S-transferase M1, T1 and P1 genetic polymorphisms with the risk of prostate cancer in various populations. The current study was done with Iranian subjects to evaluate the...

Author(s): Sahar B. Ansari, R. Vasudevan, Ali Bakhshi, M. Mirinargesi, I. Patimah, A. R. Sabariah and P. Pasalar

October 2010

Proteomics-based dissection of biotic stress responsive proteins in bread wheat (Triticum aestivum L.)

Proteomic techniques that allow the identification and quantification of stress-related proteins, mapping dynamics of their expression and post translational modifications represent an important approach in the research of plant stresses. Biotic stress is one of the major stresses limiting crop productivity and the geographical distribution of many important crops worldwide. Two hundred and seventeen protein spots...

Author(s): Abu Hena Mostafa Kamal, Ki-Hyun Kim, Kwang-Hyun Shin, Da-Eun Kim, Myeong-Won Oh, Jong-Soon Choi, Hisashi Hirano, Hwa-Young Heo and Sun-Hee Woo

October 2010

Assessment of somaclonal variation for salinity tolerance in sweet potato regenerated plants

Genetic variation is the source for plant breeding. Somaclonal variation is genetic variation induced during tissue culture and also during ordinary growth in vivo, and occurs rather, often in sweet potato. The aim of the present study was to evaluate the degree of somaclonal variation in regeneration via somatic embryogenesis by phenotypic analysis under salinity stress condition and to assess the potential of...

Author(s): Nadia Anwar, Akira Kikuchi and Kazuo N. Watanabe

October 2010

Genetic diversity among populations in Scotch pine (Pinus silvestris L.) seed stands of Western Black Sea Region in Turkey

The aim of this study was to determine the differences among some of the registered seed stands in the Western Black Sea Region in Turkey by means of the morphological characters. The number of 18 morphological characters were designated by measuring and observing on seed (seed length, seed width, seed color and 1000 seed weight), juvenile seedling (root length, hypocothyl length, epycotyl length, cotyledon number,...

Author(s): Hakan Sevık, Sezgin Ayan, Ibrahim Turna and Zeki Yahyaoglu

October 2010

Effect of space flight factors on alfalfa seeds

To explore the effect of space flight factors on the early development of alfalfa seedling, dry seeds were placed onboard a satellite for a 15-day flight. After retrieval, the ultra structure of seed coat and the chemical content of seed were tested, followed by tests for germinate ability, seedling growth, and mitotic and chromosome aberrations. Results showed that space flight factors have both positive and negative...

Author(s): WeiBo Ren, Yunwei Zhang, Bo Deng, Huiqin Guo, Libo Cheng and Yaxue Liu

October 2010

Molecular characterization and similarity relationships among sunflower (Helianthus annuus L.) inbred lines using some mapped simple sequence repeats

Information about the genetic diversity and relationships among breeding lines and varieties is not only useful for germplasm conservation and inbred line identification, but also for the selection of parental lines for quantitative trait loci (QTL) mapping as well as hybrid breeding in crops, including sunflower. In order to develop mapping populations, genetic distances among twenty eight sunflower genotypes were...

Author(s): R. Darvishzadeh, M. Azizi, H. Hatami-Maleki, I. Bernousi, B. Abdollahi Mandoulakani, M. Jafari, and A. Sarrafi

October 2010

Isolation of volatile compounds of Aloe excelsa (Berger)

Industrial and pharmacological applications of volatile and non-volatile compounds isolated from plants have been dominating the commercial sector over the recent two decades. Attempts in isolation of volatile compounds of aloes have impact on the medicinal as well as the cosmetic industries. Volatile compound isolation and extraction was performed on leaf exudates of Aloe excelsa. Three commercially important...

Author(s): R. M. Coopoosamy

October 2010

Prevalence of Vibrio cholerae in rivers of Mpumalanga province, South Africa as revealed by polyphasic characterization

Cholera is a life-threatening diarrhoeal disease, which mainly affects inhabitants of developing countries due to poor socio-economic conditions and lack of access to potable water and sanitation. Toxigenic Vibrio cholerae are the aetiological agents of cholera. These bacteria are autochthonous to aquatic environments, hence water plays a central role both in the epidemiology and transmission of cholera. The...

Author(s): Evelyn Madoroba and Maggy N. B. Momba

October 2010

Dynamics of photosynthesis in Eichhornia crassipes Solms of Jiangsu of China and their influencing factors

With LI-6400 portable photosynthesis system, the photosynthetic characteristics of artificially cultured Eichhornia crassipes in Jiangsu, China, were monitored from June 1 to November 14, 2009. Both the net photosynthetic rate (Pn) in differentpositions and light and temperature-response curves of the top fourth leaf were measured in an open-circuit gas channel system in June, July, and...

Author(s): Li Xia, Zheng Jianchu, Yan Shaohua, Ren Chenggang, Wang Man, Cong Wei, Sheng Jing and Zhu Puping

October 2010

Statistical screening of factors affecting glucoamylase production by a thermotolerant Rhizopus microsporus TISTR 3518 using Plackett-Burman design

Glucoamylase is a key enzyme used in food processing as well as in commercial production of glucose from starch. The use of thermotolerant strain of Rhizopus microsporus TISTR 3518 offers the advantages of cooling-costs reduction during fermentation and high thermostable enzyme production. The effect of various carbon and nitrogen sources on glucoamylase production was evaluated. It was found that...

Author(s): Jantima Arnthong, Boonpa Wanitchaploy,  Kenji Sakai,  Jean-Jacques Sanglier and Vichien Kitpreechavanich,

October 2010

Potential cyclodextrin glycosyltransferase producer from locally isolated bacteria

Cyclodextrin glycosyltransferase (CGTase) is one of the most important groups of microbial amylolytic enzymes that have been used for degradation of starch to yield cyclodextrin (CD) via cyclization reaction. The increasing demand for CD in industrial application has led to an extensive study about CGTase which begin with screening, isolation and characterization of CGTase-producing bacteria. The identification of...

Author(s): Norhayati Ramli, Suraini Abd-Aziz, Mohd Ali Hassan, Noorjahan Alitheenand Kamarulzaman Kamaruddin

October 2010

Optimization of physico-chemical and nutritional parameters for pullulan production by a mutant of thermotolerant Aureobasidium pullulans in fed batch fermentation process

A mutant of thermotolerant Aureobasidium pullulans was isolated using growth inhibitors viz. nystatin and deoxyglucose in the growth medium. An improved mutant strain, RG-5, was developed using methyl methane sulfonate (MMS) (0.5%) for 5 minutes, having killing rate of 70% level, produced 6 g l-1 higher pullulan as compared to the wild type without loosing thermotolerant and non-melanin producing ability. The mutant...

Author(s): Rajeeva Gaur and Ranjan Singh

October 2010

Biochemical changes occurring during fermentation of camel milk by selected bacterial starter cultures

The biochemical changes in amino acids, water soluble vitamins, soluble sugars and organic acids occurring during fermentation (at 43°C for 6 h) of camel milk inoculated with Streptococcus thermophilus 37, Lactobacillus delbrueckii sub sp. bulgaricus CH2, Lactococcus lactis, Lactobacillus acidophilus and mixed yogurt culture (S. thermophilus and L. bulgaricus 1:1)...

Author(s): Magdi, A. Osman, Ibrahim, E. Abdel Rahman and Hamid, A. Dirar

October 2010

Antitumor activity of tamoxifen loaded solid lipid nanoparticles on induced mammary tumor gland in Sprague-Dawley rats

An in vivo study was conducted to determine the effect of tamoxifen-loaded solid lipid nanoparticle on LA7 cell-induced rat mammary tumor. After the first tumors appearance, the thirty rats were divided into five groups. Group I served as normal control animal. The other four groups were mammary gland tumor-bearing animals treated with soy oil (Group II), tamoxifen (TAM) dissolved in soy oil (Group...

Author(s): Roghaieh Abbasalipourkabir, Aref Salehzadeh and Rasedee Abdullah

October 2010

Fluoxetine treatment for major depression decreases the plasma levels of cytokines

Elevated levels of pro-inflammatory biomarkers have been reported in major depressive disorder (MDD). The aim of this study is to investigate the plasma levels of interleukin-18 (IL-18), macrophage-inflammatory protein-1α (MIP-1α), monocyte chemoattractant protein 1 (MCP-1), stromal cell derived factor-1 (SDF-1), and regulated upon activation, normal T cell expressed and secreted (RANTES) in patients with...

Author(s): Yuedi Shen, Peilin Lu, Lili Wei, liqiang Cai, Xingyue Hu, and Wei Chen,

October 2010

Investigation of the chemical composition and biological activity of Xylopia aethiopica Dunal (Annonacae)

The phytochemical composition and physicochemical properties of oil extractable from the fruits of Xylopia aethiopica were determined. Extracts’ effects on cell membrane stability and prostaglandin synthetase activity were also evaluated. X. aethiopica oil extracted with chloroform: methanol (2:1, v/v) mixture contained carbohydrates, glycosides, flavonoids, saponins, tannins and phytosterols....

Author(s): C. N. Ezekwesili, O. F. C. Nwodo, F. U. Eneh and H. A. Ogbunugafor

October 2010

Cytotoxicity and antibacterial studies of iridoids and phenolic compounds isolated from the latex of Himatanthus sucuuba

The latex of Himatanthus sucuuba (Spruce) Woodson, used popularly in the Amazon for the treatment of tumors, gastritis, inflammations and infections, was evaluated for cytotoxicity and antibacterial activities. The iridoid lactones, plumericin and isoplumericin were isolated from latex by bioassay fractionation and were found to be associated with DNA damage. Gallic acid exhibited the highest...

Author(s): Jefferson Rocha A. Silva, Claudia M. Rezende, Angelo C. Pinto and Ana Claudia F. Amaral

October 2010

Analysis of bone mass density of lumbar spine zone of athletes

This study was carried out to evaluate T-Z scores of lumbar spine zone (L1, L2, L3, L4, L1-L4) bone mass density (BMD) of elite active male athletes in different branches and to determine the differences between them. 42 healthy male athletes aged 18 - 25 competing in different branches (Taekwondo 12, wrestling 8, Judo 10, Running 12) volunteered to take part in the study during the competition period. The measurements...

Author(s): Ibrahim Bozkurt

October 2010

Characterization of the Antheraea pernyi abnormal wing disc gene that may contribute to its temperature tolerance

De-Fu Jiang1,2, Yan-Qun Liu1,2*, Xi-Sheng Li1 and Sheng-Lin Shi2

Author(s): De-Fu Jiang, Yan-Qun Liu, Xi-Sheng Li and Sheng-Lin Shi

October 2010

Breaking the spores of Ganoderma lucidum by fermentation with Lactobacillus plantarum

Ganoderma lucidum has been utilized in medical field from ancient time. In recent studies, it is found that spores of G. lucidum consists of several bioactive substances which are much abundant than in the fruiting body. A hard sporoderm limits the absorption of these active compounds. Therefore, it is necessary to break down the sporoderm to allow complete absorption. The spore breaking methods...

Author(s): Chaiyavat Chaiyasut, Chakkrapong Kruatama and Sasithorn Sirilun

October 2010

Effects of dietary probiotic supplementation on promoting performance and serum cholesterol and triglyceride levels in broiler chicks

The aim of this study was to investigate the effect of two commercially-available probiotics, based on Enterococcus faecium and Bifidobacterium genera on performance and cholesterol and triglyceride amounts of broiler chicks' sera. One hundred and fifty-six (156), day-old, Ross chicks were randomly divided into groups A, B, and C (52 each). The birds in group A received...

Author(s): Mansour Mayahi, Mohammad Razi-Jalali and Rezvan Kiani

October 2010

Successful treatment of benign lesions by bis 3-azophenyl-4-hydroxy-6-methyl-pyran-2-one cobalt (II) in albino rats

The study of the effect of bis 3-azophenyl-4-hydroxy-6-methyl-pyran-2-one cobalt (II) as chemotherapeutic agent was undertaken with the intention of studying the effects in 7,12-dimethylbenz[a] anthracene (DMBA) and tetradecanoyl phorbol-13-acetate (TPA) induced chemical carcinogenesis on skin of 5 - 7 weeks old healthy albino rats. The animals were divided into 5 groups (A, B, C, D and E) of twenty each. The rats were...

Author(s): Samina Shahid, Shafiq ur Rehman and Ahmad Adnan

October 2010

Developmental morphology of the small intestine in Yangzhou goslings

The objective of this study was to investigate the development of the weight and the morphological development of the small intestine in Yangzhou geese. The weight, length and perimeter of the small intestine, height and width of the villi, depth of the crypts were measured when geese were 1, 14, 28, 42, 56 and 70 days of age. The results revealed that the weight of the duodenum, jejunum, and ileum (relative to the...

Author(s): B.Y. Liu, Z. Y. Wang, H. M. Yang, X. B. Wang, P. Huand J. Lu