African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12294

Full Length Research Paper

In vitro antimicrobial potential of organic solvent extracts of novel actinomycetes isolated from forest soil

M. Valan Arasu
  • M. Valan Arasu
  • Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
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T.S.Rejiniemon
  • T.S.Rejiniemon
  • Department of Zoology, Thiru Vika Government Arts College, Thiruvarur, 610 003, Tamil Nadu, India.
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N. A. Al-Dhabi
  • N. A. Al-Dhabi
  • Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
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V. Duraipandiyan
  • V. Duraipandiyan
  • Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
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P. Agastian
  • P. Agastian
  • Department of Plant Biology and Biotechnology, Loyola College, Chennai, 600 034, India.
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V.A.J. Huxley
  • V.A.J. Huxley
  • Department of Zoology, Thiru Vika Government Arts College, Thiruvarur, 610 003, Tamil Nadu, India.
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C.E. Song
  • C.E. Song
  • Lifelong Education Center, Chonnam National University, Kwangju, 500-757, Republic of Korea
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K.C Choi*
  • K.C Choi*
  • Grassland and forage division, National Institute of Animal Science, RDA, Seonghwan-Eup, Cheonan-Si, Chungnam, 330-801, Republic of Korea.
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  •  Received: 12 December 2013
  •  Accepted: 31 March 2014
  •  Published: 30 April 2014

Abstract

In vitro screening of antibacterial and antifungal activities of hexane, chloroform, ethyl acetate, methanol and water extracts of selected promising actinomycetes strains were studied towards Gram-positive, Gram-negative bacteria, dermatophytes and opportunistic pathogens. Crude antimicrobial metabolites were extracted by liquid-liquid extraction and solid-liquid extraction method using hexane, chloroform, ethyl acetate and methanol. The lowest minimum inhibitory concentration (MIC) of the extracts was assessed by the broth micro dilution method. All the extracts obtained from eight strains showed promising activity against tested Gram-positive bacteria. The hexane extracts of strain ERI-1 exhibited activity against Bacillus subtilis, Staphylococcus aureus and Staphylococcus epidermidis at a concentration of 5 mg/ml. Ethyl acetate extract of strain ERI-4 showed MIC of 5 mg/ml but S. epidermidis and S. aureus. B. subtilis exhibited activity at 2.5 mg/ml. However, ethyl acetate and lyophilized water extract of strain ERI-3 inhibited the growth of Trichophyton mentagrophytes, Trichophyton rubrum, Epidermophyton floccosum and Scropulariopsis sp at 10 mg/ml. Overall significant antibacterial and antifungal activities were noted in the ethyl acetate extract of ERI-1 and ERI-3. Methanol extract of ERI-26 exhibited good antibacterial and antifungal activities.

 

Key words: Actinomycetes, organic extracts, minimum inhibitory concentration (MIC), antimicrobial activity.