African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12488

Full Length Research Paper

Streptomyces noboritoensis isolated from rhizosphere soil and its use in controlling banana-tissue culture contaminants

Mohamed, SH1,3, El-Helafiy, SS2, Ismail Mona, A2 and Sadik, AS1,4
1Department of Biology, Faculty of Science, Taif University, P.O. Box 888, Taif, KSA. 2Department of Biotechnology, Faculty of Science, Taif University, P.O. Box 888, Taif, KSA. 3Soil, Water and Environmental Research Institute, Agricultural Research Center, 9 Gamaa st., P.O. Box 12619, Giza, Egypt. 4Department of Agricultural Microbiology, Faculty of Agriculture, Ain Shams University, P.O. Box 68 Hadayek Shubra, 11241, Cairo, Egypt.  
Email: [email protected]

  •  Accepted: 13 May 2013
  •  Published: 15 May 2013

Abstract

In this study, the role of Streptomyces noboritoensis (14) isolated from the rhizosphere of banana plant and having antagonistic activity against the bacterial- and fungal-tissue culture contaminants was determined in vitro. Results show that the filtrate was more effective against the fungal-tissue culture contaminants than the bacterial-tissue culture contaminants. This was indicated when jars were fungi-free and bacteria-free after one month and 21 days from incubation, respectively. Results of in vitro application show that the filtrate of S. noboritoensis (14) as a bio-control agent could be used for controlling the contaminants in banana. Data showed that treatment F (sterilized shoots treated with Streptomyces filtrate and cultivated onStreptomyces-inoculated medium) was the most effective followed by treatment E (sterilized shoots untreated with Streptomyces filtrate and cultivated onStreptomyces-inoculated medium). Therefore, the study suggests conducting further studies towards the use of streptomycetes in the biological control in a large scale production.

 

Key words: Streptomyces, tissue culture contaminants, antagonistic activities, bio-control.