Abstract

A study was conducted to determine the optimum concentration of plant growth regulators on in vitro callus induction of Tacca integrifolia using stem nodal segment. Fresh, dry weight and morphology of callus were evaluated and the results showed significant effects on callus induction when analyzed at a 5% level of significance. Among the treatments, Murashige and Skoog with only 6-Benzylaminopurine (1.0 mg/L) produced the highest result of fresh weight (1.2637 ± 0.14 g) and dry weight (0.1204 ± 0.01 g) and appeared the compact and green calli. Besides, the lowest results were 2,4-dichlorophenoxyacetic acid (2.5 mg/L) which only produced the fresh weight (0.2812 ± 0.04 g) and dry weight (0.0271 ± 0.00 g)  and appeared friable and yellowish. The result of this study has revealed that the presence of 2,4-Dichlorophenoxyacetic acid or 1-Naphthaleneacetic acid in combination with 6-Benzylaminopurine does not giving much impact on callus induction based on mass production in addition, while 6-Benzylaminopurine alone, can produce more calli.

Key words: White bat flower, compact callus, Dioscoreaceae, 6-Benzylaminopurine (BAP).