African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12485

Table of Content: December 2023; 22(12)

December 2023

Callus induction of Tacca integrifolia Ker Gawl using stem nodal segment

A study was conducted to determine the optimum concentration of plant growth regulators on in vitro callus induction of Tacca integrifolia using stem nodal segment. Fresh, dry weight and morphology of callus were evaluated and the results showed significant effects on callus induction when analyzed at a 5% level of significance. Among the treatments, Murashige and Skoog with only 6-Benzylaminopurine (1.0 mg/L) produced...

Author(s): Nur Azalina Azman, Asmah Awal, and Fatimah Abdol Latif

December 2023

Isolation and molecular identification of tannase producing fungi from soil

The study was conducted to isolate and identify tannase producing fungi from soil. Soil samples were collected aseptically and cultured on tannic acid agar (TAA) to screen for tannic acid hydrolysis. The observed isolates were sub-cultured to obtain pure culture and were later subjected to secondary screening in the Czapek medium. The biomass weight, tannase activity, and gallic acid concentration in the fermentation...

Author(s): Mutiat Adetayo Omotayo, Oluremi Nurudeen Olaleye, Simeon K. Odetunde and Mary Oluwatoyin Avungbeto  

December 2023

Identification of antagonistic bacterium strain and biocontrol effects on ginseng root rot disease

From healthy mountain-cultivated ginseng leaves, 45 endophytic bacteria strains were isolated. Antagonistic bacterium strain FS17 was screened with the confront culture method, and identified as Bacillus subtilis based on morphological characteristic and 16S rRNA sequence analysis. Growth rate method was used to determine that the fermentation broth of FS17 had inhibitory effect on 10 plant pathogenic fungi, and the...

Author(s): Chunyuan Zhou, Chengci Piao and Hao Zhang,

December 2023

Inoculation effectiveness of native and exotic Bradyrhizobium species strains in a Senegalese agricultural soil: A comparison on modern and traditional peanut (Arachis hypogaea L.) cultivars

Peanut is a key component of Senegal’s predominantly cereal-based farming systems, but its production is challenged by low soil fertility. Rhizobial inoculation is a promising strategy to improve crop yield and reduce the use of chemical nitrogen fertilizers. The aim of this study was to isolate the most specific and effective bradyrhizobial strain for peanut, and to determine the degree of variability in the...

Author(s): Godar Sene, Nibourou Top, Maimouna Cissoko, Nogaye Niang, Cheikh Ndiaye, Issa Faye, Mansour Thiao, Saliou Fall, and Samba Ndao Sylla,

December 2023

Preferential expression of somatic embryogenesis in five elite genotypes of Theobroma cacao (L.) associated with explant type and protocols used

This study aims to identify the suitable subculturing time, culture media (protocols) and explants type suitable for somatic embryogenesis expression in five elite genotypes of Theobroma cacao L. Thus, petals and staminodes explants extracted from immature flower buds of genotypes (C1, C8, C9, C14, and C16) are first grown in different media (CIM and CDM) to assess the subculturing time. Then, successive transfers in...

Author(s): N’goran Poh Konan Georges, Minyaka Emile, Kouassi Kan Modeste, Kone Siaka, Djeni N’dede Theodore, Kouamé Christophe and N’zi Jean-Claude