African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12193

Full Length Research Paper

14C-Glucose uptake studies in the red rot toxin treated sugarcane callus

A. B. Vedamurthy1, M. K. Sateesh2, G. R. Naik3
1Post Graduate Department of Biotechnology, The Oxford College of science, J.P Nagar, Bangalore-560078, India. 2Department of Biotechnology and Microbiology Bangalore University, Jnana Bharathi, Bangalore 560056, India. 3Department of Biotechnology Gulbarga University, Gulbarga-585106, India.
Email: [email protected]

  •  Published: 04 March 2008

Abstract

Fungal toxins cause serious damage to the cellular functions of host tissue. In the present report the toxin extracted from Colletotrichum falcatum Went was partially purified and treatments were given to the callus of susceptible sugarcane callus variety CoC 671. The influence on 14C-glucose uptake and its further utilization was investigated. The toxin treatment reduced the total uptake of glucose and also inhibited its conversion into insoluble products of cellular metabolism. Accumulation of organic acids and lowered synthesis of total sugars was mainly noticed. The susceptibility of sugarcane var. CoC 671 to red toxin seems to be due to poor efficiency and imbalance in utilization of glucose under biotic influence.

 

Key words: Colletotrichum falcatum, fungal toxin, autoradiogram, callus.

Abbreviation

PPO, 5-Diphenyl Oxazole;  POPOP, 1,4-Bis(5-Phenyl Oxazolyl) Benzene.