African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12489

Full Length Research Paper

Genetic diversity analysis of some exotic, Indian and mutant Brassica sp. through RAPD markers

S. N. Malode1*, S. M. Shingnapure1, V. N. Waghmare2 and S. Sutar2
  1P.G. Department of Botany, Government Vidarbha  Institute of Science and Humanities, Amravati-444604, India. 2Biotechnology Division, C.I.C.R., P. B. No.2, Shankar Nagar, P.O., Nagpur-440010, India.
Email: [email protected]

  •  Accepted: 02 July 2009
  •  Published: 28 June 2010

Abstract

 

Genetic make-up of Brassica crops has been playing a major contributory role towards its enhanced production. The exploitation of genetic variation among races and gene pools in oilseed Brassica is essential as considerable diversity exists in the set of plants. In the present investigation, the isolated DNA from 20 Brassicagenotypes including exotic, Indian and few mutant varieties were subjected to 40 RAPD primers among which 31 detected polymorphism. A total of 240 DNA fragments were generated by the 31 primers. Cluster analysis of 1 - 0 bivariate data using UPGMA method delineated the genotypes into 4 groups and the data was further used for constructing a dendrogram.

 

Key words: Brassica carinata, Brassica juncea, Brassica napus, RAPD markers, polymorphism, genetic diversity