African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12346

Full Length Research Paper

Influence of triadimefon on the growth and development of banana cultivars

Abdelnasser A. Galal*
  • Abdelnasser A. Galal*
  • Botany Department, Faculty of Science, Sohag University, Egypt.
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Ibrahiem A. Ibrahiem
  • Ibrahiem A. Ibrahiem
  • Genetic Engineering Laboratory, Monofia University, Egypt.
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Jehan M. Salem
  • Jehan M. Salem
  • Botany Department, Faculty of Science, Sohag University, Egypt.
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  •  Received: 11 April 2012
  •  Accepted: 17 October 2012
  •  Published: 16 April 2014


Triazole fungicide triadimefon (bayletone) is a broad systemic fungicide used in agriculture as screening agent. Triadimefon interferes with plant sterol biosynthesis leading to a changeable sterol profile, consequently morphological and cytological abnormalities. Its effect on banana cultivars was studied using shoot-tip cultures placed on Murashige and Skoog solid medium supplemented with 5 mg/L of 6-ben­zylaminopurine (BAP). The growth and proliferation of triadimefon treated shoot-tip cultures of the three-dessert banana cultivars (Hindi, Basrai and Williams) were affected compared to the control. The optimum culture conditions for root formation were obtained in the case of sub-culturing. The excised shoot cultures into Murashige and Skoog solid medium were supplemented with 1 mg/L indole-3-butyric acid (IBA). The efficiency of root system formation decreased as fungicide concentration increased. Many variations were observed among chlorophyll, carotenoids and protein contents of triadimifon (50 mg/L) treated cultures and untreated ones. High decrease was observed among the usual sterol content of triadimifon (50 mg/L) treated shoot buds compared to the control.


Key words: Banana, cultivars, fungicides, proliferation, shoot-tips, sterol biosynthesis, triadimefon, triazoles.


Abbreviations: BAP, 6-Benzylaminopurine; DMIs, sterol demethylation inhibitors; EtOH, ethanol; GLC, gas-liquid chromatography; IBA, indole-3-butyric acid; MS, Murashige and Skoog.