African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Effect of different hydrolysis methods on starch degradation

F. A. Aderibigbe*
  • F. A. Aderibigbe*
  • Department of Chemical Sciences, Ondo State University of Science and Technology, Okitipupa, Ondo State, Nigeria.
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L. A. Adejumo
  • L. A. Adejumo
  • Department of Chemical Sciences, Osun State University, Osogbo, Nigeria.
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  •  Received: 26 November 2013
  •  Accepted: 17 October 2014
  •  Published: 21 January 2015

Abstract

Hydrolysis of cassava starch was carried out using different processing routes namely: malt extract; acid; combinations of α-amylase and amyloglucosidase; combinations of acid, α-amylase and amyloglucosidase and combinations of malt extract, α-amylase and amyloglucosidase. The results of hydrolysis of all the five routes shows a wide degree of variance in their susceptibility to acid/enzyme hydrolysis in starch conversion of malt extract; acid; combinations of α-amylase and amyloglucosidase; combinations of acid, α-amylase and amyloglucosidase; combinations of malt extract, α-amylase and amyloglucosidase with dextrose equivalent (DE) of 24.29; 33.33; 73.43; 61.29; 76,74 DE, respectively, except the combinations of α-amylase and amyloglucosidase and combinations of malt extract, α-amylase and amyloglucosidase that shows very closed range. The best result was obtained with hydrolysis of the combinations of malt extract, α-amylase and amyloglucosidase which was observed to be more efficient than using any of the other routes in the present work.

 

Key words: Degradation, routes, α-amylase, glucoamylase, hydrolysis, dextrose equivalent (DE).