African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Table of Content: 21 January 2015; 14(3)

January 2015

Phytosynthesis of eco-friendly silver nanoparticles and biological applications –A novel concept in nanobiotechnology

Nano-biotechnology is an undoubtedly future generation technology which offers potential applications in multidisciplinary areas of science and technology. In the present day’s production, stabilization and utilization of nanoparticles is the eliminatory division in modern science receiving immense attention of scientists engaged in different fields of research. A number of metal nanoparticles have been engineered...

Author(s): M. Chandrasekhara Reddy, K. Sri Rama Murthy, A. Srilakshmi, K.R.S.Sambasiva Rao and T. Pullaiah

January 2015

Molecular characterization of the plum collection [Prunus domestica (L.) Borkh] of the Pedagogical and Technological University of Colombia

Eight Random Amplified Microsatellite markers (RAMs) were used to characterize the genetic diversity found in 14 Prunus materials belonging to the deciduous collection of the Pedagogical and Technological University of Colombia. A total of 121 bands were generated: they range from nine for the GT primer to 26 for the ACA primer, and have molecular weights between 100 and 2050 Kb. At a Nei-Li similarity level of 0.75,...

Author(s): Ana Cruz Morillo C., Yacenia Morillo C., Elberth Hernando P. and Ivan Adiel Avila M.

January 2015

Genetic assessment of some phenotypic variants of rice (Oryza spp.) for some quantative characters under the Gangatic plains of West Bengal

Twenty two (22) recombinant inbreed lines (RIL’s) derived from interspecific cross derivative of Oryza sativa and Oryza rufipogon along with two local check varieties MTU 7029 and Ranjit were evaluated in randomized block design (RBD) with two replications at two different environments (1st at Regional Research Station, New Alluvial Zone (NAZ), Bidhan Chandra Krishi Viswavidyalaya, Sub-Centre, Chakdah, Nadia, West...

Author(s): B. K. Senapati and Awaneet Kumar

January 2015

‘Do-sono’ passion fruit: Ecogeographical prospecting and phenotypic dispersion in transition areas between the caatinga and cerrado of Brazil

This study performed eco-geographical prospecting on five populations of wild ‘de-sono’ passion fruit plants in the rural zones of five towns from two distinct agroecological units in the state of Bahia. Ripe fruit from plants from these populations that had fallen to the ground were physicochemically characterized and had their phenotypic variability dispersion among the populations estimated via uni- and...

Author(s): Douglas de Almeida Pereira,  Ronan Xavier Correa  and Antonio Carlos de Oliveira

January 2015

Antimicrobial susceptibility assessment of compound from Aspergillus fumigatus

Ethyl acetate extract of the culture filtrate of Aspergillus fumigatus on chromatographic analysis has led to the isolation of the compound, AF-1 which exhibited a significant in vitro antimicrobial activity against the tested pathogenic microorganism. The structure of the isolated compound, AF-1 was identified as 5-hydroxy-2-(hydroxymethyl)-4H-pyran-4-one from its IR, UV, EI-MS, 13C-NMR, 1H-NMR, HMBC and HMQC data....

Author(s): Zakia Sultana Sathi, Md. Mustafezur Rahman, Muhammad Arifur Rahman, Md. AL- Faruk and Mohammad Abdur Rashid,

January 2015

Physiological properties of facultative and obligate alkalophilic Bacillus sp. strains isolated from Saudi Arabia

Isolation and identification of new alkalophilic Bacillus strains have increasing interest due to their possessing valuable and commercially interesting enzymes. To date, several researchers have studied the identification and characterization of alkalophilic Bacillus strains based on the phenotypic characterization as phylogenetic analysis of 16SrRNA. In the present study, six obligate and facultatively alkalophilic...

Author(s): Assaeedi Abdulrahman

January 2015

Phytochemical screening and antimicrobial activities of the leaf extract of Entandrophragama angolense

The phytochemical screening and antimicrobial activities of the leaf extract of Entandrophragma angolense were investigated using the agar well diffusion method. The methanolic extracts at crude level were shown in vitro to inhibit Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli and Streptococcus cremoris with diameter zones of inhibition ranging from 6 to 14 mm. Minimum inhibitory concentration was 3.13...

Author(s): G. A. Shittu and E. S. Akor

January 2015

Enhancement of biomass production and nutrition utilization by strain Lactobacillus acidophilus DGK derived from serial subculturing in an aerobic environment

Successive subculturing under aerobic conditions for 14 days could be used to improve characteristics of a parent strain with a growth defect. Identification of the derived strain was based on the API 50 CHL test kit and partial sequencing of the hsp60 gene. Results show that an aerotolerant strain, Lactobacillus acidophilus DGK, was created with improved nutrition utilization....

Author(s): Chin-Fa Hwang, Chien-Ku Lin, Shin-Yi Yan, Ren-Han Chang and Hau-Yang Tsen

January 2015

Induction and characterization of pathogenesis-related proteins in roots of cocoyam (Xanthosoma sagittifolium [L] Schott) infected with Pythium myriotylum

Although Pythium myriotylum is a very destructive root pathogen of cocoyam, the host defense response in this plant-pathogen interaction has not been fully studied. Four cocoyam germplasm accessions were inoculated with P. myriotylum, and their induced defense responses were characterized. The induction and spatio-temporal accumulation of chitinase and b-1,3-glucanase were determined by enzymatic activity assays of...

Author(s): Leopold M. Nyochembeng and Caula A. Beyl

January 2015

Effects of Moringa oleifera Lam. aqueous leaf extracts on follicle stimulating hormone and serum cholesterol in Wistar rats

The study evaluated the effect of Moringa oleifera aqueous leaf extracts on follicle stimulating hormone and serum cholesterol in Wistar rats. Thirty six (36) mature Wistar rats (20 male and 16 female rats) were used. The male rats were grouped into four groups with five animals each, while the female animals were grouped into four made up of four animals per group, on the basis of their body weights. Graded doses (1, 5...

Author(s): Nwamarah, Joy Ugo, Otitoju, Olawale and Otitoju, Grace T. O.

January 2015

Effect of different hydrolysis methods on starch degradation

Hydrolysis of cassava starch was carried out using different processing routes namely: malt extract; acid; combinations of α-amylase and amyloglucosidase; combinations of acid, α-amylase and amyloglucosidase and combinations of malt extract, α-amylase and amyloglucosidase. The results of hydrolysis of all the five routes shows a wide degree of variance in their susceptibility to acid/enzyme hydrolysis...

Author(s): F. A. Aderibigbe and L. A. Adejumo

January 2015

Quality characterization of Niger seed oil (Guizotia Abyssinica Cass.) produced in Amhara Regional State, Ethiopia

In the present investigation, an attempt has been made to find out cholesterol and total free fatty acid content in Niger seed oil which is the most available edible oil in Ethiopia. Acid value, peroxide value, saponification value and cholesterol content were determined. The analysis performed using Liebermann-Burchard method revealed no level of cholesterol content. Unlike the peroxide value, acid value and...

Author(s): Dimberu G. Atinafu, Shamshad K. B. and Solomon Libsu