Abstract

A dominant male sterility (DGMS) line 79-399-3 was developed from spontaneous mutation in Brassica oleracea var. capitata and has been widely used in the production of hybrid cultivar in China. In this line, male sterility is controlled by a dominant gene Ms-cd1. In the present study, primary mapping of Ms-cd1 was conducted by screening a segregating population developed by four times backcrossing of B. oleracea var. alboglabra into a male sterile B. oleracea var.italica line harboring Ms-cd1. Bulked segregation analysis (BSA) was performed for 226 BC₄ individuals using SRAPs regarding of male sterility and fertility. Using 800 SRAP primers and 2,340 SRAP combined random primers, a primary map surrounding Ms-cd1 was constructed. Eight markers closely linked to the target gene were identified, among which the closest one on each side to Ms-cd1 was 0.53 and 5.04 cM, respectively. Markers linked closely to the Ms-cd1 gene will enrich resources of molecular marker of Ms-cd1 locus; also serve to lay the foundations for molecular-assisted selection in breeding program, as well as fine mapping and map-based cloning of Ms-cd1 gene.

Key words: Sequence-related amplified polymorphism, DNA markers, linkage map, Chinese kale.

Abbreviation

GMS, Genic male sterility; DGMS, dominant male sterility; NMS,nuclear male sterility; CMS, cytoplasmic male sterility; SRAP, sequence-related amplified polymorphism; CTAB, cetyltrimethylammonium bromide; BSA, bulked segregant analysis; MAS, marker-assisted selection; SNP, single nucleotide polymorphism; Ms-cd1, male sterility dominant gene. PCR, polymerase chain reactions; RAPD, random amplified polymorphic DNA; ORFs, open reading frames;SSR, simple sequence repeat; SCAR, sequence characterized amplified region;ERPAR, extended random primer amplified region; AFLP, amplified fragment length polymorphism.