African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12278

Full Length Research Paper

The use of histological analysis for the detection of somatic embryos in sugarcane

Melo Emanuelle Ferreira
  • Melo Emanuelle Ferreira
  • Laboratory of Plant Cell and Tissue, Department of Plant Science, University of Viçosa, CEP: 36570-000, Viçosa, MG, Brazil.
  • Google Scholar
Ramos Rachel Soares
  • Ramos Rachel Soares
  • Laboratory of Plant Cell and Tissue, Department of Plant Science, University of Viçosa, CEP: 36570-000, Viçosa, MG, Brazil.
  • Google Scholar
Melo Cristiane Gamarano
  • Melo Cristiane Gamarano
  • Laboratory of Plant Cell and Tissue, Department of Plant Science, University of Viçosa, CEP: 36570-000, Viçosa, MG, Brazil.
  • Google Scholar
Rodrigues Caroline Ribeiro
  • Rodrigues Caroline Ribeiro
  • Department of Forestry, University of Viçosa, CEP: 36570-000, Viçosa, MG, Brazil.
  • Google Scholar
Vieira Marcone Sabino
  • Vieira Marcone Sabino
  • Laboratory of Plant Cell and Tissue, Department of Plant Science, University of Viçosa, CEP: 36570-000, Viçosa, MG, Brazil.
  • Google Scholar
Barbosa Márcio Henrique Pereira
  • Barbosa Márcio Henrique Pereira
  • Laboratory of Plant Cell and Tissue, Department of Plant Science, University of Viçosa, CEP: 36570-000, Viçosa, MG, Brazil.
  • Google Scholar


  •  Received: 30 May 2013
  •  Accepted: 02 December 2013
  •  Published: 05 February 2014

Abstract

The aim of this study was to establish an in vitro system for the induction, maturation and regeneration of somatic embryo in sugarcane from buds of cultivar RB 867515. Embryogenic calluses were obtained on semi-solid MS medium supplemented with 4.42 mg L-1 2,4-D. After four weeks of culture of explants on the callus induction medium, globular structures were obtained. At the end of 20 days in maturation medium, somatic embryos were observed. Histological analysis showed somatic embryos with caulinar and root apex, protodermal tissue, and the vascular system, which apparently has no connection with the vascular tissue explant that gave rise to it confirming the presence of the somatic embryo. The embryos were transferred to regeneration medium containing 1 mg L-1 GA3 and BAP, and after 1 to 2 weeks of culture, green points were observed, indicating the beginning of the formation of shoots.

 

Key words: Saccharum spp, bud culture, 2.4-D, morphogenetic pathway, embryogenesis, plant regeneration.