African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12213

Table of Content: 5 February, 2014; 13(6)

February 2014

Nanotechnology in sustainable agriculture: Present concerns and future aspects

Nanotechnology is a promising field of interdisciplinary research. It opens up a wide array of opportunities in various fields like medicine, pharmaceuticals, electronics and agriculture. The potential uses and benefits of nanotechnology are enormous. The current global population is nearly 7 */billion with 50% living in Asia. A large proportion of those living in developing countries face daily food shortages as a...

Author(s): Ram Prasad, Vivek Kumar and Kumar Suranjit Prasad

February 2014

Plant regeneration in eggplant (Solanum melongena L.): A review

Eggplant is highly responsive to various tissue culture techniques. Somatic embryogenesis and direct organogenesis are widely studied protocols in this crop, but potential of regeneration varies with genotype, explant and culture media supplemented with different combination and concentration of growth hormones. The genotype is the most important factor affecting somatic embryogenesis and organogenesis. Embryogenic...

Author(s): M. K. Sidhu, A. S. Dhatt and G. S. Sidhu

February 2014

Comparative effects of some medicinal plants on blood glucose concentration and lipid levels in alloxan-induced diabetic rats

The comparative effects of the chloroform extracts of the leaves of Psidium guajava (Myrtaceae), Anacardium occidentale (Anacardiaceae) and Eucalyptus globulus (Myrtaceae) and fruits of Xylopia aethiopica (Annonaceae) on blood glucose concentration and lipid levels of diabetic rats were investigated using standard methods. The results show 74, 82 and 83% reductions in the blood glucose concentrations upon the...

Author(s): GOMETI, Ajiroghene S., OGUGUA, Victor N., ODO, Christian E. and JOSHUA, Parker E.

February 2014

Quantitative trait loci analysis for seed yield and component traits in sunflower

The present investigation was carried out to identify the molecular markers associated with various characters in sunflower using recombinant inbred lines. Linkage analysis was carried out and five linkage groups were obtained with 19 simple sequence repeats (SSR) markers. Linkage map construction, single marker analysis (SMA) and composite interval mapping analysis were carried out with SSR primers and quantitative...

Author(s): J. Vanitha, N. Manivannan and R. Chandirakala

February 2014

Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) and its usefulness in soil microbial ecological studies - A review

The reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) is a highly specific polymerase chain reaction (PCR) method that allows one to detect very low transcription levels of functional gene(s) in soil. RT-qPCR helps us to know the active members of the microbial community, and their activities can be linked with other ecological processes in soil. If after the extraction of RNA from soil, the mRNA is...

Author(s): Stella Asuming-Brempong

February 2014

Diversity analysis of sweet potato (Ipomoea batatas [L.] Lam) germplasm from Burkina Faso using morphological and simple sequence repeats markers

Collecting and characterizing plant material has been basic for crop improvement, and diversity has long been seen as vital for rational management and use of crops. Thirty (30) morphological characters and thirty (30) simple sequence repeat (SSR) markers were used to assess the diversity among 112 sweet potato (Ipomoea batatas [L.] Lam) cultivars in Burkina Faso and to develop a core collection. Eight morphological...

Author(s): Somé Koussao, Vernon Gracen, Isaac Asante, Eric Y. Danquah, Jeremy T. Ouedraogo, Tignegre Jean Baptiste, Belem Jerome and Tarpaga M. Vianney

February 2014

Ashwagandha (Withania somnifera L. Dunal) crop as affected by the application of farm yard manure (FYM) and inorganic phosphorus in typic Torripsamment of Hisar

The vegetative parameters of ashwagandha (viz. plant height, number of primary branches, plant spread and dry weight of shoot) were enhanced significantly with the application of 12.5 mg P2O5 kg-1 soil, whereas; dry weight of roots was enhanced up to the application level of 25 mg P2O5 kg-1 soil. FYM at the rate of 12.5 t ha-1 in combination with 12.5 mg P2O5 kg-1 soil significantly improved all the vegetative...

Author(s): Vishal Goel and B. S. Duhan

February 2014

Genomic DNA extraction method from Annona senegalensis Pers. (Annonaceae) fruits

Extraction of DNA in many plants is difficult because of the presence of metabolites that interfere with DNA isolation procedures and downstream applications such as DNA restriction, replications, amplification, as well as cloning. Modified procedure based on the hexadecyltrimethyl ammoniumbromide (CTAB) method is used to isolate DNA from tissues containing high levels of polysaccharides. The procedure is applicable to...

Author(s): Cletus Anes UKWUBILE

February 2014

The use of histological analysis for the detection of somatic embryos in sugarcane

The aim of this study was to establish an in vitro system for the induction, maturation and regeneration of somatic embryo in sugarcane from buds of cultivar RB 867515. Embryogenic calluses were obtained on semi-solid MS medium supplemented with 4.42 mg L-1 2,4-D. After four weeks of culture of explants on the callus induction medium, globular structures were obtained. At the end of 20 days in maturation medium, somatic...

Author(s): Ramos Rachel Soares, Melo Emanuelle Ferreira,  Melo Cristiane Gamarano, Rodrigues Caroline Ribeiro, Vieira Marcone Sabino and Barbosa Márcio Henrique Pereira

February 2014

Safety and efficacy of a Labisia pumila var alata water extract on sexual well being and lipid profile of pre- and postmenopausal women: A randomized double-blind pilot study

This randomized double-blind, placebo-controlled study investigated the safety and efficacy of Labisia pumila (LP) water extract on sexual health, lipid profile and inflammatory markers in 36 healthy pre-and post-menopausal North American women. Participants were randomized to either LP (200 mg) or placebo for 12 weeks. The female sexual function index (FSFI) and short form-36 health survey (SF-36) were completed, and...

Author(s): Annie George, Dale Wilson, Azreena Abas and Malkanthi Evans

February 2014

Resistance-related gene transcription and antioxidant enzyme activity in Nicotiana spp. resistant to anthracnose

The two tobacco relatives of Nicotiana alata and Nicotiana longiflora display a high level of resistance against Colletotrichum nicotianae and the two genes NTF6 and NtPAL related to pathogen defense transcription were higher in N. alata and N. longiflora than the commercial cv. K326. Inoculation with C. nicotianae reduced the abundance of NTF6 and NtPAL transcript during the first 48 h post inoculation (hpi) when only...

Author(s): Jiang Dingxin, Wang Lijin, Chen Zepeng and Wei Jianyu

February 2014

Potentiality of benthic dinoflagellate cultures and screening of their bioactivities in Jeju Island, Korea

Eleven strains of benthic dinoflagellates (Amphidinium carterae (D1), Prorocentrum rhathymum (D2), Symbiodinium sp. (D3), Coolia malayensis 1 (D4), Ostreopsis ovata 1 (D5), Ostreopsis ovata 2 (D6), Coolia malayensis 2 (D7), Amphidinium operculatum 1 (D8), Heterocapsa psammophila (D9), Coolia malayensis 3 (D10) and Amphidinium operculatum 2 (D11)) were collected in Jeju Island, Korea and cultured in 20 L carboys after...

Author(s): Md. Mahfuzur Rahman Shah, Kalpa W. Samarakoon, Ju-Young Ko, H. H. Chaminda Lakmal, Ji-Hyeok Lee, So-Jeong An, You-Jin Jeon and Joon-Baek Lee