African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12269

Full Length Research Paper

Screening of some Zygomycetes for cellulase activity

Abolfazl Lotfi2, Mohammad Ali Tajick Ghanbary1*, Gholam Ali Ranjbar2 andAhmad Asgharzadeh3
1Department of Plant Protection, Sari Agricultural and Natural Resources University Sari, Iran. 2Department of Biotechnology, Sari Agricultural and Natural Resources University Sari, Iran. 3Department of Soil Biology, Soil and Water Researches Institute, Tehran, Iran.
Email: [email protected], [email protected]

  •  Accepted: 30 December 2009
  •  Published: 31 July 2010

Abstract

This study was aimed to screen the cellulytic ability of some genera ofZygomycetes under laboratory conditions. Carboxy methyl cellulose (CMC) and Wheat straw (WS) were used as the only carbon source in a minimal culture medium. Four days after inoculation, released proteins and sugars were assayed with related reagents and repeated each, 3 days up to the 31th day. Statistical analysis showed significant variation in released sugars but no significant variation in released proteins among tested genera. Mucor hiemalis f. corticola had highest and Mucor circinelloides f. circinelloides had lowest sugar levels. Glucose levels forM. hiemalis f. corticola increased until 16 days after inoculation, then decreased until 25th day, but had no variation until 30th day. These results showed that isolates belonging to the same forms had no significant difference in cellulase activity, but the ability of different genera and species were noticeable. This study also showed that WS medium can be effectively used for cellulase production by fungi.

 

Key words: Cellulase activiy, carboxy methyl cellulose, wheat straw, protein assay, sugar assay.