African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12193

Full Length Research Paper

Development of a high-throughput microsatellite typing approach for forensic and population genetic analysis of wild and domestic African Bovini.

B.J. Greyling1,2*, P. Kryger1,5, S. du Plessis2, W.F. van Hooft1,6, P. van Helden3, W.M. Getz1,4 and A.D.S. Bastos1
1Mammal Research Institute, Department of Zoology and Entomology, University of Pretoria, Pretoria 0002, South Africa. 2Agricultural Research Council, Irene, South Africa. 3Division of Molecular Biology and Human Genetics, University of Stellenbosch, South Africa. 4Department of Environmental Science Policy and Management, University of California at Berkeley, USA. 5Department of Integrated Pest Management, Research Centre Flakkebjerg, Denmark 6Resource Ecology Group, Wageningen University, Droevendaalsesteeg 3a, building nr. 100, 6708 PB, Netherlands 
Email: [email protected]

  •  Accepted: 23 January 2008
  •  Published: 04 March 2008

Abstract

Conservation management and forensic traceability of African buffalo and cattle rely on the timely provision of unbiased and accurate genetic information. An approach in which 17 cattle microsatellite markers are co-electrophoresed, following amplification in three core multiplex reactions was established for this purpose. Mean allelic richness per locus was 8.24 and 6.47, for buffalo and Bonsmara cattle, respectively, whilst an unbiased match probability of 6.5x10-17and 1.03 x 10-16 was obtained for each. These results confirm the usefulness of this rapid, cost-effective typing approach for forensic, paternity and fine-scale genetic analyses of wild and domestic African Bovini tribe members.

 

Key words: African buffalo, microsatellite, forensic, traceability, typing, Bovini, Africa.