African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12184

Full Length Research Paper

Extraction of high quality RNA from hard tissues of adult coconut palms

Ramon Souza-Perera
  • Ramon Souza-Perera
  • Instituto Tecnológico Superior de los Ríos. Km. 3 carretera Balancán-Villahermosa, Balancán 86930, Tabasco, México.
  • Google Scholar
Nelson Torres-Hernandez
  • Nelson Torres-Hernandez
  • Unidad de Recursos Naturales, Centro de Investigación Científica de Yucatán. Calle 43 No. 130, Chuburná de Hidalgo, Mérida 97200, Yucatán, México.
  • Google Scholar
Jose Juan Zuniga-Aguilar
  • Jose Juan Zuniga-Aguilar
  • Instituto Tecnológico Superior de los Ríos. Km. 3 carretera Balancán-Villahermosa, Balancán 86930, Tabasco, México.
  • Google Scholar


  •  Received: 25 January 2014
  •  Accepted: 12 August 2016
  •  Published: 28 February 2018

Abstract

An experimental protocol originally designed to isolate plant DNA was modified to obtain high quality total RNA from organs of adult coconut palms collected in situ. With this protocol, high quality RNA was extracted from leaves, inflorescences, primary and secondary roots, zygotic embryos and solid endosperm, with no carbohydrate or protein contamination. Reverse transcription-polymerase chain reaction (RT-PCR) amplification of a 470 bp cDNA, corresponding to a highly conserved domain of the eukaryotic mitogen-activated protein kinases, demonstrated the integrity of the RNA samples. Isolation of intact RNA from coconut palms growing under wild conditions facilitates the study of gene regulation ex vivo.
 
Key words: Coconut palms, RNA extraction, secondary metabolites.

Abbreviation

CTAB, Cethyl-trimethyl-ammonium bromide; PVP, polyvinylpyrrolidone; FL, flag leaf; IF, immature inflorescence; SR, secondary root; ZE, zygotic embryo; SE, solid endosperm, MAPK, mitogen-activated protein kinase.