African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Response of French bean genotypes to Colletotrichum lindemuthianum and evaluation of their resistance using SCAR markers

Antony Kimani Kamiri
  • Antony Kimani Kamiri
  • Department of Agricultural Resource Management, University of Embu, P.O Box 6-60100, Embu, Kenya.
  • Search for this author on:
  • Google Scholar
Edith Esther Arunga
  • Edith Esther Arunga
  • Department of Agricultural Resource Management, University of Embu, P.O Box 6-60100, Embu, Kenya.
  • Search for this author on:
  • Google Scholar
Felix Rotich1 and
  • Felix Rotich1 and
  • Department of Agricultural Resource Management, University of Embu, P.O Box 6-60100, Embu, Kenya.
  • Search for this author on:
  • Google Scholar
Reuben Otsyula
  • Reuben Otsyula
  • Non-Ruminant Research Institute, Kenya Agricultural and Livestock Research Organization, Kakamega, P.O. Box 169-50100, Kakamega, Kenya.
  • Search for this author on:
  • Google Scholar


  •  Received: 10 November 2020
  •  Accepted: 10 December 2020
  •  Published: 28 February 2021

 ABSTRACT

Common bean anthracnose caused by Colletotrichum lindemuthianum is one of the major biotic constraints to production of French beans (Phaseolus vulgaris L.) in Kenya. This study aimed at screening French bean genotypes in relation to their response to common bean anthracnose in order to identify potential sources of germplasm for breeding. The genotypes were tested in three sites (Kakamega, Mwea and Kutus) where results revealed a significant genotype by environment interaction effect (p ≤ 0.05) which emphasizes the strong influence of the growing conditions on the expression of host resistance. Physiological characterization identified a total of 14 distinct races out of 16 successfully plated isolates, revealing a very high diversity of C. lindemuthianum in Kenya. Six races have not been reported in previous studies in the country and are considered as new races, that is, races 84, 141, 246, 515, 576 and 768. Andean race 401 was the most virulent race with a virulence index of 67% among the genotypes. Molecular analysis using six sequence characterized amplified region (SCAR) markers revealed polymorphism among the genotypes. The SCAR markers SBB-14, SH-18 and SAB-03 have shown to be useful for marker assisted selection (MAS) of the target resistance genes. This study has also identified locally improved breeding lines as potential donors for resistance breeding to C. lindemuthianum in Kenya.

 

Key words: Anthracnose, sequence characterized amplified region (SCAR), marker assisted selection (MAS).


 INTRODUCTION

French bean (also referred to as the snap or green bean) is valued for its production of tender, thin pods containing small seeds (Singh et al., 2015). It is a type of common bean (Phaseolus vulgaris L.), a crop grown widely as a source of protein (Broughton et al., 2003; Blair et al., 2007). The immature legumes have pods that are very tender and thin, with small seeds, features that make them to be considered as vegetables (Allaire  and  Brady, 2010). French bean is valued for its protein (23%); the pods are also rich in calcium (Ca), phosphorous (P) and iron (Fe). They are packed with dietary fiber which contains high levels of vitamin A, a strong antioxidant that fights against high cholesterol, heart disease and cancer. They also contain vitamin B6 (pyridoxine), thiamin (vitamin B-1), and vitamin C (Anderson et al., 2009). Locally in Kenya the green leaves are also used as vegetables (Mwaninki, 2017).
 
Anthracnose caused by the Ascomycete Colletotrichum lindemuthianum (Sacc. & Magn.) Scrib is among the main diseases of the common bean (Phaseolus vulgaris L.) in Kenya (Balardin et al., 1997). The seed-borne pathogen is a major production constraint affecting common beans worldwide, especially in areas that experience high relative humidity and moderate temperatures (Singh and Schwartz, 2010; Conner et al., 2019). Severe infections of anthracnose in common bean can lead to almost complete crop loss because the pathogen produces masses of conidia that are capable of being easily disseminated to healthy tissues (Fernández et al., 2000; Sharma et al., 2008; Padder et al., 2017). Successful dissemination of the spores to the pods produces lesions that reduce the marketable yield of French beans (Singh et al., 2015). From the pods, the inoculum that is deposited on the seed acts as a main source of inoculum in the subsequent crop (Melotto and Kelly, 2000).
 
Management of the pathogen can be achieved through clean seed programs although this is not a viable option for many farmers in Africa because they lack well organized dry bean seed production systems (Ferreira et al., 2013). The French bean seed systems are well organized resulting in low incidences of anthracnose transmission through seed, although inoculum transmission from neighboring dry bean farms is likely to occur in susceptible cultivars. In this regard, majority of farmers in Kenya mainly rely on fungicides to reduce production and post-harvest losses to this disease (Wahome et al., 2011).  The continued use of chemicals leads to emergence of disease resistant pathogen races, increased production costs and negative effect on the environment and human health (Burkett-Cadena et al., 2008).  Several other control measures have been applied including cultural methods such as crop rotation, intercropping, elimination of plant debris, adjustment of planting dates, use of compost, and blending heterogeneous cultivars which have shown to reduce disease severity (Deeksha et al., 2009). However, these management practices come with several challenges and therefore,  the  use  of  host  plant  resistance  is the most economical and environmentally  sustainable  method  for controlling anthracnose of beans.
 
The successful development of anthracnose resistant cultivars depends on the understanding of the levels of variability in the pathogen and in the host.  Populations of C. lindemuthianum comprise a collection of races, as recognized by their interaction with known major genes for host resistance (Batureine, 2009). A set of twelve differential varieties have been established as a means of characterizing the race structure of any given population of C. lindemuthianum; some of these races have arisen in the host’s Andean gene pool, and others in the Mesoamerican gene pool  (Melotto and Kelly, 2000; Gonçalves-Vidigal et al., 2009). Currently, nearly 250 races have been identified, 74 of which have been recorded in Kenya (Mogita et al., 2011a; Musyimi, 2014; De Lima et al., 2017). The dynamic race structure of C. lindemuthianum populations has implications for the deployment of resistance genes, particularly in Africa where the majority of farmers are unable to purchase pathogen-free seeds or fungicides (Otsyula et al., 2004; Mogita et al., 2011b).
 
Anthracnose resistance in common bean is governed by monogenic independent genes that are identified by the Co symbol (Kelly and Vallejo, 2004), although quantitative resistance loci (QRL) has also been reported (Oblessuc et al., 2014; González et al., 2015). Seventeen numbered genes (Co-1 to Co-17) and other unnumbered genes (Co-u, Co-w, Co-x, Co-y, Co-z, CoPv02c3-X, CoPv02c7-X, CoPv02c19-X, CoPv0c2449-X and CoPv09c453-C) have been mapped in common bean (Geffroy et al., 2008; Lacanallo et al., 2010; Ferreira et al., 2013; Campa et al., 2014; Coimbra‐Gonçalves et al., 2016; Zuiderveen et al., 2016). The resistance in all the loci is dominant except for the recessive co-8 gene. Furthermore, co-localization of the anthracnose resistance gene and genes for angular leaf spot and rust resistance have been reported offering additional resistance to other pathogens (Gonçalves-Vidigal et al., 2011; Sousa et al., 2015; Valentini et al., 2017).
 
The mapping of the Co genes has majorly been achieved using molecular markers which offer an opportunity for marker-assisted selection (MAS) to enhance anthracnose resistance in common bean. A number of sequence characterized amplified polymorphism (SCAR) markers, random amplified polymorphic DNA (RAPD) markers and single nucleotide polymorphism (SNP) markers that are tightly linked to anthracnose resistance genes have been reported (Young   and   Kelly,   1997;   Alzate-Marin   et  al.,  2000; Melotto and Kelly, 2000; Garzón et al., 2008; Vallejo and Kelly, 2009). These markers offer new opportunities for the identification of genes for resistance and have been used successfully in common bean breeding programs (Miklas et al., 2003; Ragagnin et al., 2003; Njuguna, 2014). This study was therefore undertaken to determine the response of Kenyan French bean germplasm to C. lindemuthianum under natural environment, to determine their resistance to local races and analyze these entries for their banding patterns to molecular markers that are linked to specific anthracnose resistance genes. This information is important to unlock opportunities for the development of French bean cultivars with complex resistance through MAS.


 MATERIALS AND METHODS

Plant material
 
The germplasm panel was made up of 33 entries comprising 16 commercial French bean genotypes commonly grown in Kenya, 3 local breeding lines, 2 landraces and the 12 standard differential cultivars for C. lindemuthianum. The French bean seed was sourced from various seed companies, research organizations and the National Gene bank of Kenya; while the seeds for the differential cultivars were sourced from the International Center for Tropical Agriculture (CIAT-Uganda). The differential cultivars were used as checks in the study because their gene pools and resistance genes have been documented (Kelly and Vallejo, 2004; Ferreira et al., 2013).
 
Field screening experimental sites
 
The plant materials were planted at three sites (Mwea, Kutus and Kakamega) in Kenya, chosen to provide a contrasting set of environments. Mwea and Kutus represent French bean growing zones in Kenya, while Kakamega represented a bean disease hot spot site based on previous studies in Kenya (Mogita et al., 2011b; Arunga et al., 2012; Kimno et al., 2016). The three sites experience a bimodal pattern of rainfall: the long rains season from March to June and the short rains season from September to December. The Kakamega site, located at the Non-Ruminant Research Institute (34°46´E, 0°16´N, elevation of 1555 meters above sea level), receives an annual rainfall of 1950 mm per year while the local mean temperature is 21°C.  The experiments in Mwea were set up at the Kenya Agricultural and Livestock Research Organization (KALRO) Center in Mwea (37°20´E, 0°37´S; elevation of 1159 meters above sea level), with a total annual mean rainfall of about 850 mm and a mean air temperature of 22°C. The experiments in Mwea and Kakamega were set up during the long and short rain seasons in 2017. During the short season, the disease incidence in Mwea was extremely low and therefore, another site (Kutus), representing another French bean growing zone was included during the long rains of 2018.  Kutus and Mwea sites are found in Kirinyaga County, a major French bean production region in Kenya. The Kutus site (35°37’E, 0°01´S; elevation of 1287 meters above sea level) climate is classified as tropical. The average annual temperature   is   21°C and receives   1095 mm rainfall annually.
 
Experimental layout and data analysis
 
The experiments were set out using a randomized complete block design, with three replicates included in each environment. Each entry was represented by a 4.8 m long single row at a spacing of 45 cm between rows and 15 cm between plants. The dry bean cultivar GLP 585 was used as a guard row. Disease severity was assessed on a whole plot basis, and was quantified using the 1-9 CIAT scale as described  by Pastor-Corrales et al., (1998), in which a score of 1-3 is taken to indicate a high level of resistance, 4-6 a moderate level of resistance and 7-9 susceptibility (Table 1). A disease severity index (DSI) was calculated as follows: (n1 + 2*n2 + 3*n3 +4 *n4 + 5*n5 + 6*n6 + 7*n7 + 8*n8 + 9*n9)/N, where 1 through 9 represent the disease score of individual plants and n1 through n9, the number of plants exhibiting a specific severity score (Ombiri et al., 2002). N represents the total number of plants examined per entry. The disease severity index (DSI) data collected from the field was subjected to analysis of variance (ANOVA) using routines implemented in SAS 9.4 software for Windows (Version 9.4. Cary, NC: SAS Institute Inc; 2014), considering four environments (Kakamega season 1, Kakamega season 2, Mwea and Kutus) and 34 entries as fixed factors. Means were compared using the Tukey’s honestly significant difference metric at a confidence level of 95%.
 
Physiological characterization of C. lindemuthianum
 
A total of 16 diseased leaf samples with signs of attack, that is, lower leaf surface with brick red to purplish red discoloration on the leaves and dark brown eyespots on the pods (Figure 1a) were collected from the four contrasting enviroments. Furthermore, purposeful sampling was employed to collect eight more samples from farmers’ fields around the study sites making a total collection of 16 samples. These samples were transferred to University of Embu Research Laboratory for isolation as described by Pastor-
Corrales et al.  (1998).
 
Isolates of C. lindemuthianum collected from the field were cultured on Potato Dextrose Agar (PDA) media (Figure 1b). For single spore isolation, successful colonies were plated on tap water agar (TWA) for 3 days and single hyphae picked under a stereo microscope and cultured on new PDA media. Colonies that failed to sporulate on PDA were cultured on V8 media to enhance sporulation. Incubation was done for 21 days at 22°C in alternating 12 h of light and darkness. Inoculum was prepared from 21 days old monospore cultures by adding 5 ml of distilled water on the surface of the culture and scrapped gently with a brush. Thereafter, the spore suspension was sieved using a cheese cloth and the inoculum was adjusted to a concentration of 1.2 x 106 spores/ml using a hemocytometer (Mahuku et al., 2002).
 
For race identification, screening of differential cultivars was carried out in a screen house. For each of the isolate, 12 differential cultivars were sown in seedling trays of five seedlings per row in three replicates. The growing media was made up of loam soil, manure and sand in the ratio of 2:1:1, respectively. Fourteen days after sowing, plants were inoculated with monosporic cultures of C. lindemuthianum. Using a small sprayer, the spores were sprayed on the stem and leaves of the plants until runoff. After inoculation, the plants were incubated and maintained in a moist chamber (20–22ËšC, 95% relative humidity) for three days, and thereafter, maintained at 20-28ËšC for 7 days. After this period, the disease symptoms were scored visually for anthracnose severity index on the leaves using a CIAT scale of 1-9 (Pastor-Corrales et al., 1998).  Identification of the races was based on  the  susceptibility  of  each differential cultivar to the isolates of the pathogen using a binary nomenclature as described by Pastor-Corrales (1991). In summary, each differential cultivar has an assigned number (2n), where n corresponds to the order number of the cultivar within the 12 standard bean differential series (Table 2). The race designation was obtained as the sum of all binary numbers of cultivars with susceptible reactions.
 
 
Screening French bean accessions for resistance to different races of C. lindemuthianum
 
Identified races in the differential cultivars were re-isolated to obtain monosporic cultures by plating the samples on TWA and single hyphae obtained under microscope. Each single hyphae was plated on PDA and V8 media for sporulation for 21 days at 22°C in alternating 12 h of light and darkness.  In order to screen the French bean genotypes, a modified detached leaf technique was used as described by Tu (1986). The detached leaf technique was earlier used to confirm the races of the collected samples and results obtained were similar to the analysis based on whole plants and therefore the method was suitable for screening the French bean accessions. The modified detached leaf technique involved inoculating detached leaves in clean Petri dishes in the laboratory. The procedure started with preparing clean plastic Petri dishes by lining them with a thin layer of cotton that was moistened with sterile water. In the evening hours (cool weather), young trifoliate leaves were carefully excised from three weeks old seedlings by pinching with a finger at the petiole. They were then placed in triplicates in Petri dishes with the lower sides of the leaves facing up. The monosporic spore suspension was sieved using a cheese cloth, and the inoculum was adjusted to a concentration of 1.2 x 106 spores/ml using a hemocytometer for each race. The inoculum was sprayed gently onto the leaves using a hand sprayer. The Petri dishes were then placed on the research laboratory tables (University of Embu) at room temperature (25 ± 2°C) for 10 days. The leaves were moistened daily using sterile water to prevent the leaves from wilting. The leaves were assessed on the 10th day post-inoculation for anthracnose severity by scoring the pathogen reaction from three trifoliate leaves (Figure 2) on a scale of 1-9 (Pastor-Corrales et al., 1998).
 
 
Molecular analysis of French bean genotypes using SCAR markers
 
Seeds of the French bean accessions and the differential cultivars were sown in plastic pots in a greenhouse maintained at 25 ± 5°C at the University of Embu, Kenya. The growth media comprised of loam soil, manure and sand in the ratio of 2:1:1, respectively. Each pot was supplied with Diammonium Phosphate (DAP) fertilizer. The pots were watered daily to field capacity.  For DNA extraction, three-week old young leaves for each French bean genotype and differential cultivars were collected. DNA was extracted according to Mahuku and Riascos (2004). The DNA samples were then amplified using six SCAR markers that are linked to anthracnose genes that have some levels of resistance in Kenya (Table 3). A total PCR reaction volume of 10 μl was used composing of 5 ηg/ul DNA, 20uM of each specific reverse and forward primer, 5x Bioline MyTaq Reaction buffer (5 mM dNTPs, 15 mM, MgCl2, stabilizers and enhancers), 1.2 units of Taq DNA Polymerase (Bioline) made up to the volume using molecular grade water. PCR reactions were performed using the following regime: an initial hybridization step at 95°C for 1 min; followed by 35 cycles of denaturation step at 95°C for 30 s, annealing step ranging from 60-65°C for 1 min (Table 5), a third extension step at 72°C for 2 min, followed by a final extension at 72°C for 5 min. After amplification, a volume of 2 µl of 6x DNA loading dye  (New  England  Biolabs-NEB) was added  to  each amplicons and resolved on 1.2% agarose gel containing ethidium bromide, run in 1x Sodium borate buffer at 100 volts for 2 h. The DNA bands were viewed under ultraviolet light and analyzed as binary data by recording 1 and 0 for presence and absence of the marker, respectively.
 


 RESULTS AND DISCUSSION

Reaction of French beans and differential cultivars to anthracnose under field conditions
 
According to the ANOVA, there were significant differences (p≤0.05) in the severity of anthracnose infection both among the genotypes and environments. The significant genotype by environment interaction effect emphasizes the strong influence of the growing conditions on the expression of host resistance which is agreement with work conducted by Kiptoo et al. (2020). The highest mean DSIs were experienced at Kakamega (long rains season: 4.0, short rains seasons: 2.64), with a lower disease pressure experienced at Mwea (1.71) and Kutus (1.64). In the Kakamega long rains season experiment, 10 of the 17 French bean genotypes revealed moderate resistance (mean DSI 4-6) while 4 were completely susceptible (7-9). Only 3 genotypes showed resistance (1-3) (Table 4). The entries which showed consistency with respect to resistance were the landrace GBK032921, three breeding lines (T19, MU#2 and MU#13) and two commercial French bean cultivars (Julia and Monel). Among the differential cultivars, Perry Marrow (Co-13), Kaboon (Co-12), Widusa (Co-15, Co-33), TU (Co-5), AB136 (Co-6, co-8) and G2333 (Co-42, Co-52, Co-35) showed the most consistent level of resistance across the four environments. Differentials of the Mesoamerican gene pool exhibited high anthracnose resistance compared to those of the Andean gene pool. Similar results have been observed for other common bean pathogens in Kenya and East Africa (Kimno et al., 2016; Chilagane, 2017). The resistance exhibited across the different environments by differential cultivars Widusa, AB136 and G2333 is likely to reflect their harbouring of several Co genes, since their resistance  is effective against multiple races of the pathogen (Alzate-Marin et al., 1997; Gonçalves-Vidigal et al., 1997; Young et al., 1998; Alzate-Marin et al., 2000; Vallejo and Kelly, 2009; De Lima et al., 2017). It is therefore important to consider gene pyramiding in resistance breeding against anthracnose.
 
 
 
The French bean varieties currently grown in Kenya expressed little resistance to anthracnose.  Nevertheless, the resistance expressed by some commercial cultivars, landraces and local breeding materials could be exploited as a basis for breeding French beans because, in French beans, yield is a complex trait brought about by additional pod quality traits (Singh et al., 2015). Furthermore, the differential cultivar Widusa, which is also a French bean from Europe (Drijfhout and Davis, 1989), can be exploited as a resistant donor in Kenya. Widusa is classified as an Andean differential cultivar but pedigree evidence suggests that it could be of Mesoamerican origin (Gonçalves-Vidigal and Kelly, 2006), supporting the importance of Mesoamerican genes in anthracnose resistance in Kenya. Further, ANOVA depicted significant environment main effect and a significant genotype by environment interaction effect which emphasizes the strong influence of the growing conditions on the expression of host resistance. This is likely driven by the effect of the environment on the growth and dispersal of the pathogen.  The high DSIs observed at the Kakamega site during the long rains season probably reflect the prevalence of warm, humid conditions coupled with high rainfall during the growing season. The ideal conditions for the growth of the C. lindemuthianum pathogen are an air temperature range of 13-26°C, the availability of abundant moisture and frequent rainfall which encourages the dispersion of conidia (Schwartz et al., 2005; Sharma et al., 2008). In contrast, Mwea and Kutus sites experience a much lower rainfall and are generally less humid, conditions which are less favourable for the development of anthracnose. These results therefore support the studies by Kimno et al. (2016) who recommended Kakamega as a hotspot for screening common bean diseases.
 
Physiological characterization of C. lindemuthianum
 
Evaluation using specific races was considered important in this study given the confounding effects brought about by other pathogens as observed in the field. Race characterization   will   also help one to understand the composition of races in the country to assist in breeding for resistance since anthracnose exhibits a vertical/qualitative form of resistance (Miklas et al., 2006). The current study identified 14 distinct races from 16 successfully plated isolates revealing a high level of diversity of C. lindemuthianum out of which 43% were considered new races in Kenya (Table 5). Similar results have been identified in the neighboring country, Tanzania where 96% of the 42 races were considered new obtained from 50 isolates (Mpeguzi et al., 2020). Previous studies conducted in Kenya by Musyimi (2014) identified 12 races most of them being different from the races that were obtained in the other studies too. Race 65 may be considered as widespread as it was identified in Kakamega and Mwea. Races 65 and 73 have also been documented to be prevalent in Brazil (Vieira et al., 2018). Furthermore, race zero (0) was also identified in the present study as it could not infect any of the twelve anthracnose differential cultivars as previously reported in Kenya (Mogita et al., 2011b)and Uganda (Nkalubo, 2006). 
 
There is a need therefore, to review the current set of differential cultivars to include a susceptible cultivar in the differential set that can distinguish this race especially in Eastern Africa which is considered to be a secondary center of diversity for common beans (Allen and Edje, 1990; Wortmann, 1998; Sperling, 2001).
 
The race composition in the current study was predominantly Andean (70%). This could be probably due to a large number of French bean genotype used in the study that have been reported to be largely of the Andean origin (Arunga et al., 2015, Arunga and Odikara, 2020). In addition, the common bean composition in the country has been reported to be of Andean origin as well (Asfaw et al., 2009). This supports the work  carried   out   by  Baradin  and  Kelly   (1998) where they discovered that races isolated from Andean cultivars have a broader virulence on germplasm of the Andean gene pool and a reduced virulence on Mesoamerican varieties, which suggests a co-evolution between the pathogen and the host. Among the differential cultivars, the most affected cultivar was Michelite having been overcome by seven (7) of the 14 isolates with cultivars AB136 and G2333 being the most resistant as they were not affected by any of the isolates. The susceptibility observed in cultivar Michelite has also been reported to be situated on the lower tire of the differential cultivars as a susceptible check that also succumbed to seven isolates. Similar to field results, high resistance was observed in the Mesoamerican genotypes as compared to the Andean genotypes with the exemption of the cultivar Kaboon (Co-1). Race characterization revealed that cultivar MDRK succumbed to five races including race 768, first to be reported in the country. Resistance exhibited by the cultivars AB136 and G2333 is likely to reflect their harbouring of several Co genes.
 
Virulence of 14 races of C. lindemuthianum on French bean genotypes
 
The evaluated French bean genotypes showed different virulence reactions to the 14 C. lindemuthianum races used in this study (Table 6). Races 401 and 84 were the most virulent across all the genotypes while race 768 was the least virulent race. Among the French bean cultivars, Lomami was the most susceptible cultivar as it was infected by 86% of the races; whereas cultivar Boston, breeding lines MU#3, MU#13 and T19 were the most resistant. The results for the breeding lines demonstrated that the greenhouse/laboratory results were not very different from the field experiment. The breeding lines were resistant to most Mesoamerican races except for Andean races 2, 38 and 84. These breeding lines were obtained from one breeding program which utilized resistance genes from both gene pools including dry beans (Arunga et al., 2015). This probably resulted in the introgression of the Mesoamerican genes into the French bean background.
 
Among the differential cultivars, the high susceptibility observed in cultivar Michelite was as expected given that the cultivar is situated on the lower tire of the differential cultivars as a susceptible check. Similar to field results, race characterization revealed high resistance among the differential cultivars of the Mesoamerican origin compared to those of the Andean origin with the exemption of the cultivar Kaboon. Cultivar Kaboon was only susceptible to two Andean races in the current study, that is, races 33 and 55. Susceptibility to race 55 has been reported in previous studies (Alzate-Marin et al., 1997). According to Melotto and Kelly (2000), the major dominant resistance gene present in the cultivar Kaboon is an allele of the Co-1 gene, called Co-12. The authors revealed the importance of this gene in common bean breeding programs, as it gives resistance to both Andean and Central American races recently identified in Michigan. Similarly, cultivar Kaboon was resistant under field conditions across the four environments. Given the great composition of the French bean genotypes in Kenya are of the Andean origin, this cultivar can be used for gene introgression to improve resistance to C. lindemuthianum. Andean differential genotypes possess locus (Co-1), the only most utilized locus in the cultivars of this origin conferring resistance to C. lindemuthianum (Melotto and Kelly, 2000; Zuiderveen et al., 2016). This could be the bottleneck facing genotypes within this gene pool. The resistance exhibited by the cultivars AB136 and G2333 is likely to reflect their harbouring of several Co genes.
 
Further, the study employed a better, simple and rapid screening method/technique to ensure efficient selection of resistant materials. Detached leaf technique has been deployed in the past as an alternative to conventional screening techniques in screen houses and has been reported to be successful with similar results (Rezene et al., 2018). This is particularly important where seeds are limited and there is a need to preserve the whole plant for further evaluation. The method can also be carried out in the lab reducing the need for installation of screen houses. In common beans, the method has been used in screening for ALS and anthracnose (Tu, 1986; Rezene et al., 2018). The technique has also been employed in other crops such as maize against southern corn leaf blight (Aregbesola et al., 2020), soybean against white mold (Twizeyimana et al., 2007)and Fusarium in wheat (Browne and Cooke, 2004).
 
Molecular markers analysis
 
All the six (6) SCAR markers used in this study generated polymorphisms between each parent and the resistant and susceptible genotypes which were specific for the loci to which they are linked. However, only SAB-03, SZ-20 and SH-18 markers were allele-specific (Table 7). The marker SY-20830bps a dominant marker reported to be very specific and tightly linked to the Co-4 locus at 0.0 cM (Costa et al., 2010), could not discriminate the other alleles at this locus among the 12 differential set. This is in agreement with similar work done by Beraldo et al., (2009). Figure 3C shows the clear band of cultivar TO (Co-4) (No 9), the tagged locus for the marker and the thicker bands of the alternative alleles possessed by cultivars PI 207262 (Co-43) and G2333 (Co-42) No 8 and12 respectively. This is in agreement with Vieira et al. (2018) who did similar work in Brazil. The marker however, did not detect the possible gene for resistance among the French bean genotypes used in this study.
 
 
Linkage to the locus Co-6 in chromosome Pv 07 was evaluated using the marker SZ-20845 bps linked at a distance of 7.1 cM derived from the differential cultivar AB 136 (Queiroz et al., 2004). The marker was only identified with the differential cultivar AB 136 revealing its specificity (Figure 3D). Cultivars Mitchelite (Co-11), MDRK (Co-1), Kaboon (Co-12), TO (Co-4), TU (Co-5) and G2333 (Co-35, Co-42and Co-5) amplified an unspecific band close to the locus. Among the French bean genotypes, the marker was detected in three cultivars Edge, Blazer and T19 (Tables 6 and 7).
 
The locus Co-6 has been known to offer broad-based resistance to most of the Andean races in the Latin America (23, 31, 64, 69, 73, 81, 89 and 453) but less effective against races of Mesoamerican origin (Gonçalves-Vidigal et al.,1997;Gonçalves-Vidigal et al., 2001). Thus, the Co-6 locus can be utilized in breeding programs for   French   beans   in   Kenya   after   successful marker validation. The SCAR marker SAS-13900bps tightly linked to the Co-42 locus at 0.4 cM (Young et al., 1998; Kelly et al., 2003), was detected in two differential cultivars PI 207262 (Co-43) and G2333 (Co-42) specific to the tagged locus and one unspecific amplification in differential cultivar Widusa (Co-15, Co-33) (Figure 3A). Of the nine French bean genotypes under evaluation identified by the only local French bean breeding lines were consistent under field   evaluation   and   on   the reaction with specific races. Recent studies have shown that SAS-13900c is not specific for Co-4 allele (Awale and Kelly, 2001; Alzate-Marin et al., 2007) but, has been known to detect Co-4 locus consistently regardless of the type of allele as was confirmed in this study.
 
 
The SCAR marker SBB-141050/1150bps a codominant marker also linked to the gene Co-42 revealed consistency in detecting the Co-4 locus among  the  three differential   cultivars   PI 207262  (Co-43)  to  (Co-4)   and G2333 (Co-42) (Figure 3E). However, amplification of cultivars Cornell 49-242 (Co-2) and AB136 (Co-6 and Co-8) was unusual and none of the other genotypes were detected. The marker SH-181100bps a dominant marker revealed consistency in detecting the Co-42 locus among the differential cultivars PI 207262 (Co-43) and G2333 (Co-42); however, none of the other genotypes was identified with the marker. The Co-4 locus is the most utilized locus in common bean for anthracnose resistance. Its allele (Co-42) has been shown to provide greater resistance as compared to the original Co-4 and Co-43 allele and is among the broadest-based resistance genes described in common bean (Balardin and Kelly, 1998; Silvério et al., 2002; Vaz Bisneta and Gonçalves‐Vidigal, 2020).
 
SAB-03400bps maker that tags the gene Co-5 at a distance of 5.9 cM (Vallejo and Kelly, 2001)was detected in the anthracnose differential cultivars TU (Co-5) and G2333 (Co-5) as expected (Figure 3B). The marker was detected in  eight  other  genotypes,  that  is,  five  French beans cultivars, two breeding lines and one local cultivar (Table 7). Only the local breeding lines in the MU series revealed consistency with the marker both in the field and response to specific races.
 
The Co-5 gene has not been widely deployed in the past in resistance breeding programs but, has been identified as one of the most effective genes in a survey of races of C. lindemuthianum from Central America and Mexico that is predominantly Mesoamerican (Balardin et al., 1997). However, the combination of Co-5 and Co-4 has been seen as the most effective in providing broadest resistance to C. lindemuthianum races. Thus, the comparative advantage of the limited use of Co-5 in breeding makes the locus even more valuable to breeders for gene pyramiding bean programs.
 
SCAR markers tightly linked to anthracnose resistance genes offer another means to detect specific genes and for marker assisted selection as has been revealed in this study. Combination of Co-42 and Co-5 has been shown to offer the highest and broad resistance spectrum to anthracnose as revealed  in  cultivar  G2333 which agrees with the worked done by Sousa et al., (2015).  The consistency of the local breeding lines with the markers could be traced to the fact that these breeding lines were obtained from one breeding program which utilized resistance genes from both gene pools including dry beans (Arunga et al., 2015). This may have led to the introgression of the Mesoamerican resistant genes into the French bean background that has been known to offer broader resistance as compared to the Andean genotypes. Four of the six SCAR markers tested in the present work (SH18 and SBB-14 for Co-42, SAB-03 for Co-5, and SY-20 for Co-4), have shown to be useful for assisted selection of the target resistant genes. They were specific for the loci to which they are linked, even though some did not discriminate alleles from the same locus, except for SH18, which was shown to be specifically linked to Co-42. However, more robust molecular markers such as SSRs, CAPS and SNPs should be developed for future selection of French bean genotypes for resistance to C. lindemuthianum as the current set of SCAR markers were only useful to very few French bean genotypes in Kenya.


 CONCLUSION

The current study has revealed that, the race composition of C. lindemuthianum in Kenya is continuously changing and highly variable, hence the need for continuous evaluation.  The highest composition of the races was of the Andean origin that could probably be due to the large number of French beans used in this study that are predominantly Andean, evidence for co- evolution between C. lindemuthianum and the host within the two centers of origin. Local breeding lines in the MU series could offer a good basis of gene introgression in Kenya, a great advantage in reducing linkage drag common with introgression from dry beans. Identification of race zero (0) in this study is another evidence that there is a need to review the current set of differential cultivars to include a susceptible cultivar in the differential set that can distinguish this race. Use of molecular markers proved effective and can assist future breeding efforts focusing in identification of other possible resistance loci to buffer against resistance breakdown through gene pyramiding especially in combination with both field evaluation and race characterization.


 ACKNOWLEDGMENT

The author acknowledges Kirkhouse Trust for financial support and Kenya Agricultural and Livestock Research Organization (KALRO), Kakamega for field experiments. While the research reported here was funded by Kirkhouse Trust, the design, execution and interpretation of the research remains wholly the responsibility of the authors.


 CONFLICT OF INTERESTS

The authors have not declared any conflict of interests.



 REFERENCES

Allaire H, Brady T (2010). Classification and botanical description of legumes. Retrieved from

View
 

Allen D, Edje O (1990). Common bean in African farming systems. Paper presented at the Progress in improvement of common bean in Eastern and Southern Africa. Network on Bean Research in Africa Workshop Series.

  
 

Alzate-Marin AL, Baía GS, De Paula TJ, De Carvalho GA, De Barros EG, Moreira MA (1997). Inheritance of anthracnose resistance in common bean differential cultivar AB 136. Plant Disease and Genetics 81(9):996-998.
Crossref

  
 

Alzate-Marin AL, Menarim H, Chagas JM, Barros EGd, Moreira MA (2000). Identification of a RAPD marker linked to the Co-6 anthracnose resistant gene in common bean cultivar AB 136. Genetics and Molecular Biology 23(3):633-637.
Crossref

  
 

Aregbesola E, Ortega-Beltran A, Falade T, Jonathan G, Hearne S, Bandyopadhyay R (2020). A detached leaf assay to rapidly screen for resistance of maize to Bipolaris maydis, the causal agent of southern corn leaf blight. European Journal of Plant Pathology 156(1):133-145.
Crossref

  
 

Arunga EE, Odikara OS (2020). Characterization of Kenyan French Bean genotypes into gene pool affiliations using allele specific markers. African Journal of Biotechnology 19(9):653-660.
Crossref

  
 

Arunga EE, Kinyua M, Ochuodho J, Owuoche J, Chepkoech E (2015). Genetic diversity of determinate French beans grown in Kenya based on morpho-agronomic and simple sequence repeat variation. Plant Breeding and Crop Science 7:240-250.
Crossref

  
 

Arunga EE, Ochuodho J, Kinyua M, Owuoche J (2012). Characterization of Uromyces appendiculatus isolates collected from snap bean growing areas in Kenya. African Journal of Agricultural Research 7(42):5685-5691.
Crossref

  
 

Asfaw A, Blair MW, Almekinders C (2009). Genetic diversity and population structure of common bean (Phaseolus vulgaris L.) landraces from the East African highlands. Theoretical Applied Genetics 120(1):1-12.
Crossref

  
 

Awale HE, Kelly JD (2001). Development of SCAR markers linked to Co-4^ 2 gene in common bean. Annual Report-Bean Improvement Cooperative 44:119-120.

  
 

Balardin R, Jarosz A, Kelly J (1997). Virulence and molecular diversity in Colletotrichum lindemuthianum from South, Central, and North America. Phytopathology 87(12):1184-1191.
Crossref

  
 

Balardin RS, Kelly JD (1998). Interaction between Colletotrichum lindemuthianum races and gene pool diversity in Phaseolus vulgaris. Journal of the American Society for Horticultural Science 123(6):1038-1047.
Crossref

  
 

Batureine MJ (2009). Diversity of Colletotrichum lindemuthianum and reaction of common bean germplasm to anthracnose disease. (B.Sc. AGR- Hons: Crop Option), Makerere University, Uganda.

  
 

Beraldo ALA, Colombo CA, Chiorato AF, Ito MF, Carbonell SM (2009). Use of SCAR markers in common bean breeding for anthracnose resistance. Bragantia 68(1):53-61.
Crossref

  
 

Blair MW, Porch T, Cichy K, Galeano CH, Lariguet P, Pankhurst C, Broughton W (2007). Induced mutants in common bean (Phaseolus vulgaris), and their potential use in nutrition quality breeding and gene discovery. Israel Journal of Plant Sciences 55(2):191-200.
Crossref

  
 

Broughton WJ, Hernandez G, Blair M, Beebe S, Gepts P, Vanderleyden J (2003). Beans (Phaseolus spp.)-model food legumes. Plant Soil 252(1):55-128.
Crossref

  
 

Browne R, Cooke B (2004). Development and evaluation of an in vitro detached leaf assay forc pre-screening resistance to Fusarium head blight in wheat. European Journal of Plant Pathology 110(1):91-102.
Crossref

  
 

Burkett-Cadena M, Kokalis-Burelle N, Lawrence K, Van Santen E, Kloepper J (2008). Suppressiveness of root-knot nematodes mediated by rhizobacteria. Biological Control 47(1):55-59.
Crossref

  
 

Campa A, Rodríguez-Suárez C, Giraldez R, Ferreira JJ (2014). Genetic analysis of the response to eleven Colletotrichum lindemuthianum races in a RIL population of common bean (Phaseolus vulgaris L.). BMC Plant Biology 14(1):115.
Crossref

  
 

Chilagane LA (2017). Genotype-pathogen characterization and markers identification for angular leaf spot disease resistance in common bean in Tanzania. Sokoine University of Agriculture.

  
 

Coimbra‐Gonçalves GK, Gonçalves‐Vidigal MC, Coelho RT, Valentini G, Vidigal Filho PS, Lacanallo GF, Sousa LL, Elias HT (2016). Characterization and mapping of anthracnose resistance gene in Mesoamerican common bean cultivar Crioulo 159. Crop Science 56(6):2904-2915.
Crossref

  
 

Conner RL, Gillard CL, Mcrae KB, Hwang SF, Chen YY, Hou A, Penner WC, Turnbull GD (2019). Survival of the bean anthracnose fungus (Colletotrichum lindemuthianum) on crop debris in Canada. Canadian Journal of Plant Pathology 41(2):209-217.
Crossref

  
 

Costa M, Tanure J, Arruda K, Carneiro J, Moreira M, Barros E (2010). Development and characterization of common black bean lines resistant to anthracnose, rust and angular leaf spot in Brazil. Euphytica 176(2):149-156.
Crossref

  
 

De Lima CSA, Gonçalves-Vidigal MC, TA, Santana Gilio, Lacanallo GF, Valentini G, Martins Vanusa dSR, Song Q, Galván Marta Z, Hurtado-Gonzales OP, Pastor-Corrales MA (2017). Genetics and mapping of a new anthracnose resistance locus in Andean common bean Paloma. BMC Genomics 18(1):306.
Crossref

  
 

Deeksha J, Hooda K, Bhatt J, Mina B, Gupta H (2009). Suppressive effects of composts on soil-borne and foliar diseases of French bean in the field in the western Indian Himalayas. Crop Protection 28(7):608-615.
Crossref

  
 

Drijfhout E, Davis J (1989). Selection of a new set of homogeneously reacting bean (Phaseolus vulgaris) differentials to differentiate races of Colletotrichum lindemuthianum. Plant Pathology 38(3):391-396.
Crossref

  
 

Fernández MT, Fernandez M, Casares A, Rodriguez R, Fueyo M (2000). Bean germplasm evaluation for anthracnose resistance and characterization of agronomic traits: A new physiological strain of Colletotrichum lindemuthianum infecting Phaseolus vulgaris L. in Spain. Euphytica 114(2):143-149.
Crossref

  
 

Ferreira JJ, Campa A, Kelly JD, Varshney R, Tuberosa RJ, Wiley (2013). Organization of genes conferring resistance to anthracnose in common bean. Translational Genomics for Crop Breeding. 
Crossref

  
 

Garzón LN, Ligarreto GA, Blair MW (2008). Molecular marker‐assisted backcrossing of anthracnose resistance into andean climbing beans (Phaseolus vulgaris L.). Crop Science 48(2):562-570.
Crossref

  
 

Geffroy V, Sévignac M, Billant P, Dron M, Langin T (2008). Resistance to Colletotrichum lindemuthianum in Phaseolus vulgaris: a case study for mapping two independent genes. Theoretical Applied Genetics 116(3):407-415.
Crossref

  
 

Gonçalves-Vidigal M, Cardoso AA, Vieira C, Saraiva LS (1997). Inheritance of anthracnose resistance in common bean genotypes PI 207262 and AB 136. Brazilian Journal of Genetics 20.
Crossref

  
 

Gonçalves-Vidigal M, Pedro Filho SV, Medeiros A, & Pastor-Corrales M (2009). Common bean landrace Jalo Listras Pretas is the source of a new Andean anthracnose resistance gene. Crop Science 49(1):133-138.
Crossref

  
 

Gonçalves-Vidigal MC, Cruz AS, Garcia A, Kami J, Vidigal Filho PS, Sousa LL, McClean P, Gepts P, Pastor-Corrales M (2011). Linkage mapping of the Phg-1 and Co-14 genes for resistance to angular leaf spot and anthracnose in the common bean cultivar and 277. Theoretical Applied Genetics 122(5):893-903.
Crossref

  
 

Gonçalves-Vidigal MC, Kelly JD (2006). Inheritance of anthracnose resistance in the common bean cultivar Widusa. Euphytica 151(3):411-419.
Crossref

  
 

Gonçalves-Vidigal MC, Sakiyama NS, Vidigal Filho PS, Júnior ATA, Poletine JP, Oliveira VR (2001). Resistance of common bean cultivar AB 136 to races 31 and 69 of Colletotrichum lindemuthianum: the Co-6 locus. Crop Breeding Applied Biotechnology 1(2).
Crossref

  
 

González AM, Yuste-Lisbona FJ, Rodiño AP, De Ron AM, Capel C, García-Alcázar M, Lozano R, Santalla M (2015). Uncovering the genetic architecture of Colletotrichum lindemuthianum resistance through QTL mapping and epistatic interaction analysis in common bean. Frontiers in Plant Science 6:141.
Crossref

  
 

Kelly J, Gepts P, Miklas P, Coyne D (2003). Tagging and mapping of genes and QTL and molecular marker-assisted selection for traits of economic importance in bean and cowpea. Field Crops Research 82(2-3):135-154.
Crossref

  
 

Kelly JD, Vallejo VA (2004). A comprehensive review of the major genes conditioning resistance to anthracnose in common bean. HortScience 39(6):1196-1207.
Crossref

  
 

Kimno S, Kiplagat O, Arunga E, Chepkoech E (2016). Evaluation of Selected French Bean (Phaseolus vulgaris L.) Genotypes for Resistance to Angular Leaf Spot (Pseudocercospora griseola) in Western Kenya. American Journal of Experimental Agriculture 13(4):1-6.
Crossref

  
 

Kiptoo GJ, Kinyua MG, Kiplagat OK, Matasyoh LG (2020). Incidence and severity of anthracnose (Colletotrichum lindemuthianum) on selected common bean (Phaseolus vulgaris L.) genotypes. Agricultural Research 5(84):1-12.
Crossref

  
 

Lacanallo G, Gonçalves-Vidigal M, Vidigal Filho P, Kami J, Gonela A (2010). Mapping of an Andean gene for resistance to anthracnose in the landrace Jalo Listras Pretas. Paper presented at the Annual Report of the Bean Improvement Cooperative.

  
 

Mahuku G, Jara C, Cajiao C, Beebe (2002). Sources of resistance to Colletotrichum lindemuthianum in the secondary gene pool of Phaseolus vulgaris and in crosses of primary and secondary gene pools. Plant Disease 86(12):1383-1387.
Crossref

  
 

Mahuku GS, Riascos JJ (2004). Virulence and molecular diversity within Colletotrichum lindemuthianum isolates from Andean and Mesoamerican bean varieties and regions. European Journal of Plant Pathology 110(3):253-263.
Crossref

  
 

Melotto M, Kelly J (2000). An allelic series at the Co-1 locus conditioning resistance to anthracnose in common bean of Andean origin. Euphytica 116(2):143-149.
Crossref

  
 

Miklas P, Kelly J, Beebe S, Blair M (2006). Common bean breeding for resistance against biotic and abiotic stresses: from classical to MAS breeding. Euphytica 147(1-2):105-131.
Crossref

  
 

Miklas P, Kelly J, Singh S (2003). Registration of anthracnose-resistant pinto bean germplasm line USPT-ANT-1. Crop Science 43(5):1889-1891.
Crossref

  
 

Mogita W, Ochuodho J, Gohole L (2011a). Analysis of Western Kenya Isolates of Colletotrichum lindemuthianum Casual Agent of Bean Anthracnose using Pathogenicty and BOX-A1R Analysis. African Journal of Education, Science and Technology 1(3):155-162.

  
 

Mogita W, Ochuodho J, Gohole L, Arunga E, Billy M (2011b). Incidence of bean anthracnose in Western Kenya and its management using aqueous extract of Aloe vera. African Journal of Education, Science and Technology 3(3):6-12.

  
 

Mpeguzi M, Susan NM, Mabagala R, Chilagane LA (2020). Races of Colletotrichum lindemuthianum (Sacc. Magnus) Briosi Cavara in major bean growing regions in Tanzania. African Journal of Plant Science 14(8):308-314.
Crossref

  
 

Musyimi AS (2014). Marker assisted gamete selection for multiple disease resistence in Andean Bean genotypes and characterization of Colletotrichum lindemuthianum in Kenya. (BSc Agriculture), University of Nairobi, 2014. 

  
 

Mwaninki OK (2017). Determination of pre-harvest interval for carbendazim used for control of fungal diseases in French beans. University of Nairobi.

  
 

Njuguna SM (2014). Marker Assisted Gamete Selection For Multiple Disease Resistance in Mesoamerican Bean Genotypes and Race Typing of Angular Leaf Spot Pathogen In Kenya. University of Nairobi.

  
 

Nkalubo S (2006). Study of anthracnose (Colletotrichum lindemuthianum) resistance and its inheritance in Ugandan dry bean germplasm. 

  
 

Oblessuc P, Baroni R, da Silva Pereira G, Alisson Fernando, Carbonell S, Brinez B, Luciano Da Costa ES, Garcia AA, Luis EA, Kelly JD (2014). Quantitative analysis of race-specific resistance to Colletotrichum lindemuthianum in common bean. Molecular Breeding 34(3):1313-1329.
Crossref

  
 

Ombiri J, Zinkernagel V, Gathuru E, Achwanya O (2002). First report of race 485 of Colletotrichum lindemuthianum in Kenya and its implication in bean resistance breeding. Gartenbauwissenschaft 67(3):81-85.

  
 

Otsyula R, Rachier G, Ambitsi N, Juma R, Ndiya C, Buruchara R, Sperling L (2004). The use of informal seed producer groups for diffusing root rot resistant varieties during period of acute stress. Paper presented at the Addressing seed security in disaster response: Linking relief with development.

  
 

Padder B, Sharma P, Awale H, Kelly J (2017). Colletotrichum lindemuthianum, the causal agent of bean anthracnose. Journal of Plant Pathology 99(2):317-330.

  
 

Pastor-Corrales M (1991). Standardization of differential varieties and race designation of Colletotrichum lindemuthianum. Phytopathology 81:694.

  
 

Pastor-Corrales MA, Jara C, Singh SP (1998). Pathogenic variation in, sources of, and breeding for resistance to Phaeoisariopsis griseola causing angular leaf spot in common bean. Euphytica 103(2):161-171.
Crossref

  
 

Queiroz V, de Sousa C, Costa M, Sanglad D, Arruda K, de Souza T, Ragagnin V, de Barros E, Moreira M (2004). Development of SCAR markers linked to common bean anthracnose resistance genes Co-4 and Co-6 (0084-7747).

  
 

Ragagnin VA, Sanglard DA, De Souza T, Moreira MA, De Barros EG (2003). Simultaneous transfer of resistance genes for Rust, Anthracnose and Angular Leaf Spot to cultivar Perola assited by molecular markers. Paper presented at the Annual Report-Bean Improvement Cooperative.

  
 

Rezene Y, Tesfaye K, Mukankusi C, Arunga E, Gepts P (2018). Simple and rapid detached leaf technique for screening common beans (Phaseolus vulgarise L.) in vitro against angular leaf spot (Pseudocercospora griseola) disease. African Journal of Biotechnology 17(35):1076-1081.
Crossref

  
 

Schwartz HF, Steadman JR, Hall R, Forster RL (2005). Compendium of bean diseases: American Phytopathological Society (APS Press).

  
 

Sharma P, Sharma O, Padder B, Kapil R (2008). Yield loss assessment in common bean due to anthracnose (Colletotrichum

  
 

lindemuthianum) under sub temperate conditions of North-Western Himalayas. Indian Phytopathology 61(3):323.

  
 

Silvério L, Gonçalves-Vidigal M, Filho P, Barelli MA, Thomazella C, Nunes WM (2002). Genetic resistance to Collectotrichum lindemuthianum race 2047 in G2333. Paper presented at the Annual Report-Bean Improvement Cooperative.

  
 

Singh A, Singh AP, Ramaswamy HS (2015). Effect of processing conditions on quality of green beans subjected to reciprocating agitation thermal processing. Food Research International 78:424-432.
Crossref

  
 

Singh SP, Schwartz HF (2010). Breeding common bean for resistance to diseases: a review. Crop Science 50(6):2199-2223.
Crossref

  
 

Sousa LL, Gonçalves AO, Gonçalves-Vidigal MC, Lacanallo GF, Fernandez AC, Awale H, Kelly JD (2015). Genetic characterization and mapping of anthracnose resistance of common bean landrace cultivar Corinthiano. Crop Science 55(5):1900-1910.
Crossref

  
 

Sperling L (2001). The effect of the civil war on Rwanda's bean seed systems and unusual bean diversity. Biodiversity Conservation 10(6):989-1010.
Crossref

  
 

Tu J (1986). A detached leaf technique for screening beans (Phaseolus vulgaris L.) in vitro against anthracnose (Colletotrichum lindemuthianum). Canadian Journal of Plant Science 66(3):805-809.
Crossref

  
 

Twizeyimana M, Ojiambo P, Tenkouano A, Ikotun T, Bandyopadhyay R (2007). Rapid screening of Musa species for resistance to black leaf streak using in vitro plantlets in tubes and detached leaves. Plant Disease 91(3):308-314.
Crossref

  
 

Valentini G, Gonçalves-Vidigal MC, Hurtado-Gonzales OP, de Lima Castro SA, Cregan PB, Song Q, Pastor-Corrales MA (2017). High-resolution mapping reveals linkage between genes in common bean cultivar Ouro Negro conferring resistance to the rust, anthracnose, and angular leaf spot diseases. Theoretical Applied Genetics 130(8):1705-1722.
Crossref

  
 

Vallejo V, Kelly JD (2001). Development of a SCAR marker linked to Co-5 locus in common bean. Paper presented at the Annual Report-bean Improvement Cooperative.

  
 

Vallejo V, Kelly JD (2009). New insights into the anthracnose resistance of common bean landrace G 2333. Open Horticulture Journal 2(1):29-33.
Crossref

  
 

Vaz Bisneta M, Gonçalves‐Vidigal MC (2020). Integration of anthracnose resistance loci and RLK and NBS‐LRR‐encoding genes in the Phaseolus vulgaris L. genome. Crop Science. 
Crossref

  
 

Vieira A, Almeida L, Rodrigues L, Costa J, Melo L, Pereira H, Sanglard D, Souza T (2018). Selection of resistance sources to common bean anthracnose by field phenotyping and DNA marker-assisted screening. Embrapa Arroz e Feijão-Artigo em periódico indexado. 
Crossref

  
 

Wahome S, Kimani P, Muthomi J, Narla R, Buruchara R (2011). Multiple disease resistance in snap bean genotypes in Kenya. African Crop Science Journal 19(4):289-302.

  
 

Wortmann CS (1998). Atlas of common bean (Phaseolus vulgaris L.) production in Africa: CIAT.

  
 

Young R, Melotto M, Nodari R, Kelly J (1998). Marker-assisted dissection of the oligogenic anthracnose resistance in the common bean cultivar,'G2333'. Theoretical Applied Genetics 96(1):87-94.
Crossref

  
 

Young RA, Kelly JD (1997). RAPD markers linked to three major anthracnose resistance genes in common bean. Crop Science 37(3):940-946.
Crossref

  
 

Zuiderveen GH, Padder BA, Kamfwa K, Song Q, Kelly JD (2016). Genome-wide association study of anthracnose resistance in Andean beans (Phaseolus vulgaris). PLoS ONE 11(6):e0156391.
Crossref

  

 




Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

Back to Vol. 20 No. 2
Back to articles
SUBSCRIBE TO RSS
SUBMIT MANUSCRIPT
CONFERENCES
          */?>