African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12496

Full Length Research Paper

Phytochemical and proximate composition of cucumber (Cucumis sativus) fruit from Nsukka, Nigeria

Uzuazokaro Mark-Maria Agatemor
  • Uzuazokaro Mark-Maria Agatemor
  • Department of Biochemistry, University of Nigeria, Nsukka, Enugu State, Nigeria.
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Okwesili Fred Chiletugo Nwodo
  • Okwesili Fred Chiletugo Nwodo
  • Department of Biochemistry, University of Nigeria, Nsukka, Enugu State, Nigeria.
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Chioma Assumpta Anosike
  • Chioma Assumpta Anosike
  • Department of Biochemistry, University of Nigeria, Nsukka, Enugu State, Nigeria.
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  •  Received: 25 January 2018
  •  Accepted: 15 August 2018
  •  Published: 19 September 2018

 ABSTRACT

Cucumber (Cucumis sativus L.) is very common, cultivated throughout the world and often eaten as a raw vegetable without cooking. In this study, the phytochemical and proximate compositions of cucumber were investigated. Quantitative phytochemical analysis of the homogenate of C. sativus fruit showed that reducing sugars (574.36 ± 3.88 mg/g) was highest amount when compared to other phytochemicals, alkaloids (2.22 ± 0.96 mg/g) and flavonoids (2.14 ± 0.56 mg/g) were moderately present, while cyanogenic glycoside (0.21 ± 0.13 mg/g) was the lowest in quantity. Proximate analysis showed that C. sativus fruit contained the following - fibre (1.02 ± 0.01%), moisture (94.2 ± 0.08%), protein (3.01 ± 0.07%), lipid (0.55 ± 0.13%), carbohydrate (0.28 ± 0.09%) and ash (0.94 ± 0.24%) contents.

 

Key words: Phytochemicals, Cucumis sativus, proximate constituents.


 INTRODUCTION

Phytochemicals are secondary metabolites produced by plants. These products are biologically active, naturally occurring chemicals in various parts of a plant, providing health benefits for humans further than those attributed to macronutrients and micronutrients. Their functions are diverse and include provision of strength to plants, attraction of insects for pollination and feeding, while some are simply waste products (Ibegbulem et al., 2003). They give plants colour, flavour, and smell and are part of plants’ natural defence system, protecting plants’ cells from environmental hazards such as pollution, stress, drought, UV exposure and pathogenic attacks (Ejele and Akujobi, 2011). These  compounds  have  been  linked  to human health by contributing to protection against degenerative diseases (Dandjesso et al., 2012). Phytochemicals are present in varieties of plants utilized as important components of both human and animal diets. These include fruits, seeds, herbs and vegetables (Okwu, 2005). Different mechanisms have been suggested for the action of phytochemicals. They may act as antioxidants, or modulate gene expression and signal transduction pathways (Dandjesso et al., 2012). Phytochemicals may be used as chemotherapeutic or chemopreventive agents (Paolo et al., 1991). They are formed during the plant normal metabolic processes. The medicinal values of a plant lie in its constituent phytochemicals, which produce
 
the definite physiological actions on human body. The most important of these phytochemicals are alkaloids, tannins, flavonoids and phenolic compounds (Iwu, 2000).
 
Cucumber (Cucumis sativus) fruit is a widely cultivated plant in the gourd family of Cucurbitaceae, which also includes important crops such as melon, water melon and squash (Vivek et al., 2017). The plant has large leaves that form a canopy over the fruit. The fruit of the cucumber is roughly cylindrical, elongated with tapered ends, and may be as large as 60 cm (24 inches) long and 10 cm (3.9 inches) in diameter. Having an enclosed seed and developing from flowers, botanically speaking, cucumber can be classified as an accessory fruit (Huang et al., 2009). There is increased consumption of C. sativus fruits possibly because of their high nutritional value. The nutritional compositions of C. sativus include protein, fat and carbohydrate as primary metabolites; along with dietary fibre which is important for the digestive system. C. sativus contains some essential vitamins and anti-oxidants which are effective in human health (Grubben and Denton, 2004; Wang et al., 2007). C. sativus is used for jaundice, bleeding disorders and anuria; while its seeds are highly nourishing (Gogte, 2000). Till date, the present study on C. sativus represents variety of pharmacological activities like anticancer, anthelmentic, antimicrobial, hypolipidemic, antiulcer, analgesic and antioxidant (Dhande et al., 2013). It is believed that C. sativus seed has flavonoid, tannin, terpenoids and some phytochemicals (Kumar et al., 2010). Despite the acclaimed presence of those phytochemicals, to the best of our knowledge, the phytochemical and proximate compositions of the whole fruit (homogenate) is yet to be empirically established. Here, the phytochemical and proximate compositions, along with the potential pharmaceutical function of the whole C. sativus L. fruit were examined, highlighted and shown as homogenate. 


 MATERIALS AND METHODS

Plant material
 
Cucumber (C. sativus) fruits were purchased from Nsukka Main Market, Nsukka, Enugu State, Nigeria and were identified by Mr. Alfred Ozioko of Bioresources Development and Conservation Programme (BDCP) Research Centre, Nsukka, Enugu. The fruits were washed under running water, homogenized with Kenwood high speed blender and used for analysis without further dilution.
 
Qualitative phytochemical analysis
 
The qualitative phytochemical analyses of the fruits of C. sativus were carried out according to the methods of Harborne (1998) and Trease and Evans (2002). Quantitative determination of tannin was conducted using spectrophotometric determination method described by Gupta and Verma (2010). The total phenol content of C. sativus fruit was determined using a spectrophotometric method of Wolfe et al. (2003). Cyanogenic glycoside was determined using alkaline picrate method as described by Harborne (1998).
 
Spectrophotometric determination of glycoside content was carried out with a method described by Quasheesh (1937). The flavonoid content was estimated using ferric chloride colorimetric method of Mattila and Kumpulairen (2002). Saponin content was quantitatively estimated by spectrometric determination method of Uematsu et al. (2000). Determination of alkaloid content was carried out by the method described by Harborne (1998). The amount of steroid was determined by the method described by Edeoga et al. (2005). Quantitative determination of reducing sugars was carried out using Folin and Wu method (1920). Resin content was determined quantitatively by the UV absorption method of Harborne (1998). Quantitative estimation of terpenoid content was carried out using oxidation method of Harborne (1998). Anthocyanin content was estimated quantitatively with pH differentiation method of Harborne (1998). Chlorophyll content was determined using Harborne (1998) method. 
 
 
Where, V = Volume of solvent, and W = Weight of homogenate.
 
Proximate analysis
 
The proximate analysis of the homogenate of C. sativus fruits for moisture, ash, fat and carbohydrate were determined as described by AOAC (2000). The concentration of crude protein and fibre were determined using methods described by Pearson (1976). All determinations were done in triplicates and the results were expressed as means of percent values on dry weight basis.
 
Statistical analysis
 
Each experiment was repeated three times, and the results were presented as means and standard deviation.

 


 RESULTS AND DISCUSSION

As shown in Table 1, bioactive compounds such as steroids, terpenoids, glycosides and resins were found in relatively high concentrations; saponins, alkaloids and flavonoids were present in moderate concentrations, while tannins were slightly present. Table 2 shows quantitatively, the phytochemical composition of C. sativus fruit homogenate. Bioactive compounds such as reducing sugars were found to be in highest amount (574 ± 3.88 mg/g) relatively compared to other phytochemicals as shown in Table 2. Alkaloids and flavonoids that were moderately present were found in the concentration range of 2.22 ± 0.96 and 2.14 ± 0.56 mg/g respectively. The concentration range of cyanogenic glycosides: 0.21 ± 0.13 mg/g was very low. The proximate analyses of the homogenate of C. sativus fruit showed the presence of moisture, crude protein, ash and crude fibre with values shown in Table 3. The homogenate of C. sativus fruit had high concentrations of moisture (94.6 ± 0.08%).
 
 
Proximate analyses also revealed ash as being very low (1.07 ± 0.24%).
 
This study reveals the presence of phytochemicals considered as active medicinal chemical constituents. Important medicinal phytochemicals such as terpenoids, reducing sugar, glycosides, resins, flavonoids, alkaloids, phenols, saponins, steroids and tannins were present in the homogenate of cucumber fruits (Table 1). Bioactive compounds such as reducing sugars were found to be in highest amount (574 ± 3.88 mg/g) relatively compared to other phytochemicals as shown in Table 2. Alkaloids and flavonoids that were moderately present were found in the concentration range of 2.22 ± 0.96 and 2.14 ± 0.56 mg/g, respectively. The concentration range of cyanogenic glycosides (0.21 ± 0.13 mg/g) was very low. The presence of flavonoids in cucumber fruit homogenate suggests   that  the  fruit  homogenate  has  the  ability  to scavenge free radicals as they are the chief sources of antioxidant (Singh Gill et al., 2010; Egbung et al., 2013) in plants which have been known to play some role in free radical scavenging. The antioxidant activity of the phenolics, tannins, flavonoid compounds are attributed to their redox properties which can act as reducing agents, hydrogen donors and singlet oxygen quenchers (Gulcin et al., 2007; Andreia et al., 2013). Polyphenolics having hydroxyl groups are very important plant constituents which can protect the body from different types of oxidative stress (Jing et al., 2010; Anoop and Bindu, 2015) such as CCl4 induced hepatotoxicity. Epidemiologic studies recommend that coronary heart disease is opposed by dietary flavonoids (Wadood et al., 2013). Saponins detected in the fruit, is a known anti-nutritional factor, which reduces the uptake of certain nutrients including glucose and cholesterol at the gut through intra-lumenal physiochemical interactions (Shi et al., 2004; Agbafor et al., 2015). It has been reported to have hypocholesterolemic effects; hence it is useful in human diet in controlling cholesterol levels (James et al., 2010) and may aid in lessening the metabolic burden that would have been placed on the liver during metabolism. The homogenate of C. sativus fruits show trace amount of tannins which have been reported to possess some medicinal properties (Ekeanyanwu et al., 2010). Its wound healing properties, which include anti-inflammatory, analgesic (Ayinde et al., 2007) and antioxidant properties (Okwu and Okwu, 2004) have been reported; although they (tannins) are anti-nutrients (Doss et al., 2011). Ibrahim et al. (2014) reported anti-microbial effects of tannins through membrane disruption, binding to proteins, adhesions and enzyme inhibition. This result is in line with the findings of Liener (1994) who stated that lower concentrations of tannins in plants are found to be desirable for human and animal consumption. It could be the reduced amounts of tannins in the homogenate of the fruit that enhanced the protective property rather than the side effects. The phytochemical screening result of this study is contrary to the report of Jony and Roksana (2012) who reported the absence of flavonoids in the ethanol extract of C. sativus. Perhaps also,  flavonoids  were  not  detected  as  a  result  of  the extraction method used, as Kumar et al. (2010) reported the presence of flavonoids in the aqueous extract, thus correlating the findings of this investigation. The homogenate of C. sativus fruit also revealed the presence of significant amount of chlorophylls a and b. Chlorophyll is important in many plant metabolic functions such as growth and respiration. It is used in medicinal preparation for treating anaemia and hypertension as a healing agent and in oral hygiene (Fischman, 1997; Mujoriya and Bodla, 2012), indicating that C. sativus can be used to reduce bad breath and as healing agent.
 
The homogenate of C. sativus fruit also revealed the presence of alkaloids. Plants having alkaloids are used in medicines for reducing headache and fever. These are attributed to their antibacterial and analgesic properties (Pietta, 2000; Sotiroudis et al., 2010). Alkaloids have been reported to act as central nervous system stimulant (Abubakar et al., 2015). Also, they possess antispasmodic, antifungal, anti-fibrogenic effects (Ibraheem and Maimoko, 2014). Terpenoids detected in the fruit are reported to have anti-inflammatory (Olorunju et al., 2012), anti-viral, anti-malarial, inhibition of cholesterol synthesis (Njagi et al., 2015) and anti-bacterial properties (Wadood et al., 2013). The significant amount of terpenoids and alkaloids from this study show that C. sativus fruit homogenate could be recommended as an effective source of anti-bacterial agent. The proximate analyses show that the homogenate of C. sativus fruits have high concentration of moisture and relative amount of fibre, crude protein, ash, lipid and carbohydrate. Dietary fibre helps to reduce the chance of gastro intestinal problems such as constipation and diarrhoea by increasing the weight, size and wetness of stool (Weickert and Pfeiffer, 2008; Aina et al., 2012). Plant fibres are long chain carbohydrates (polysaccharides) that are indigestible by the digestive enzymes of human gastro intestinal tract (GIT). They help to keep the digestive system healthy and also aid and speed up the excretion of waste materials from the body (Weickert and Pfeiffer, 2008). The recommended daily allowance of fibre for a healthy adult is 20-25 g/day (American Dietetic Association, 2005). The result of this study is in line with the report that C. sativus is useful in fighting constipation, as the fibre content helps to overcome the hypotonic which aids constipation (Yohanna, 2013). The low concentration of lipid obtained for the fruit homogenate suggests that its regular incorporation and consumption in the diet is healthy for people on low fat diet. The result on the high concentration of moisture agrees with the report of Aina et al. (2012) that fleshy fruits have high percentage of moisture which aids in digestion and acts as a solvent in chemical reactions in the body system. The high moisture concentration is in accordance with the report of Egan et al. (1981) and Okoye (2013) which showed the moisture content of C. sativus as 96.4 and 97.8%, respectively. The appreciable amount of ash recorded from the study (Table 3) shows that C.  sativus fruit  homogenate  could be recommended as effective sources of mineral nutrients.


 CONCLUSION

Cucumber (C. sativus) fruit is a source of the secondary metabolites, that is, alkaloids, flavonoids, terpenoids, tannins, saponins, steroids, phenols, glycosides, reducing sugars, etc. Cucumber fruit may play vital role in preventing various diseases such as inflammation, bacterial infection, lipid peroxidation, fever, constipation, etc. The anti-inflammatory, anti-bacterial, antioxidant, analgesic and anti-constipation may be due to the presence of the above mentioned phytochemicals especially flavonoid (2.14 ± 0.56 mg/g), alkaloids (2.22 ± 0.96 mg/g) and proximate constituents. Thus, it is expected that the important phytochemical properties and proximate compositions identified in this study in the igure

 


 CONFLICT OF INTERESTS

The authors have not declared any conflict of interests.

 



 REFERENCES

Association of Official Agricultural Chemists (AOAC) (2000). Official Methods of Analysis of the Association of Analytical chemists. Washington D.C. 18th Ed, pp. 12-13.
 

Abubakar I, Mann A, Matthew JT (2015). Phytochemical composition, antioxidant and anti-nutritional properties of root-bark and leaf methanol extracts of Senna alata L. grown in Nigeria. African Journal of Pure and Applied Chemistry 9(5):91-97
Crossref

  
 

Agbafor KN, Ogbanshi ME, Akubugwo EI (2015). Phytochemical screening, hepatoprotective and antioxidant effects of leaf extracts of Zapoteca portoricensis. Advances in Biological Chemistry 4:35-39.
Crossref

  
 

Aina VO, Sambo B, Zakari A, Hauwa-Haruna MS, Umar H, Akinboboye RM, Mohammed A (2012). Determination of nutritional and anti-nutrient content of Vitis vinifera (grapes) grown in Bomo (Area C) Zaria, Nigeria. Advanced Journal of Food Science and Technology 4(6):445-448.

  
 

American Dietetic Association (2005). Dietary reference intakes for energy, carbohydrate, fibre, fat, fatty acids, cholesterol, protein and amino acids. Washington D.C: National Academies Press. 

  
 

Andréia AS, Anacharis B, Adelar B, Gomes da Costa S, Eloá AK, Cristina GM, Rosane MP (2013). Hepatoprotective effects of mushrooms. Molecules 18:7609-7630
Crossref

  
 

Anoop MV, Bindu AR (2015). In vitro and anti-inflammatory activity studies on Syzgium zeylanicum (L.) DC leaves. International Journal of Pharma Research and Review 4(8):18-27.

  
 

Ayinde BA, Omogbai EK, Amaechina FC (2007). Pharmacognosy and hypotensive evaluation of Ficus exasperate Vahl (Moraceae) leaf. Acta Poloniae Pharmaceutica 64:543-546.

  
 

Dandjesso C, Klotee JR, Dougnon TV, Segbo JM, Gbaguidi F, Fah L, Fanou B, Loko F, Dramane K (2012). Phytochemistry and hemostatic properties of some medicinal plant sold as anti-hemorrhagic in Cotonou markets (Benin). Indian Journal of Science and Technology 5(8):3105-3109.

  
 

Dhande SR, Dongare PP, Shah PR, Joshi YM, Kadam VJ (2013). Antihepatotoxic potential of Cucumis sativus and Pogostemon patchouli against carbon tetrachloride induced hepatotoxicity. Indo American Journal of Pharmaceutical Research 3(11):9212-9221

  
 

Doss A, Pugalenthi M, Valivel VG, Subhashini G, Anitha SR (2011). Effects of processing technique on the nutritional composition and anti-nutrients content of under-utilized food legume Canavalia ensiformis LDC. International Food Research Journal 18(3): 965-970.

  
 

Edeoga HO, Okwu DE, Mbaebie BO (2005). Phytochemical constituents of some Nigerian medicinal plants. African Journal of Biotechnology 4(7):685-688.
Crossref

  
 

Egan H, Kirk RS, Swayer RR (1981). Pearson's Chemical Analysis of Foods. 8th Edn. Churchill Livingstone, London pp. 200-514.

  
 

Egbung GE, Atangwho IJ, Iwara IA, Odey MO, Eyong EU (2013). Chemical composition of root and stem bark extracts of Nauclea latifolia. Archives of Applied Science Research 5(3):193-196.

  
 

Ejele AE, Akujobi CO (2011). Effects of secondary metabolites of Garcinia kola on the microbial spoilage of Cajanus cajan extract. International Journal of Tropical Agricultural Food Systems 5(1):8-14.

  
 

Ekeanyanwu RC, Njoku OU, Ononogbu IC (2010).The phytochemical composition and some biochemical effects of Nigerian tigernut (Cyperus esculentus L) tuber. Pakistan Journal of Nutrition 9(7):709-715.
Crossref

  
 

Folin O, Wu H (1920). Blood sugar determination. Journal of Biological Chemistry 41:367-374.

  
 

Gogte VM (2000). Ayurvedic Pharmacology and Therapeutic Uses of Medicinal Plants. Mumbai. Chaukhamba Publisher P 633.

  
 

Grubben GJH, Denton OA. (2004). Plant Resources of Tropical Africa 2 Vegetables, Leiden, Wageningen, Backhuys Publishers, Netherlands. pp. 48-57.

  
 

Gulcin I, ElmastaÅŸ M, Aboul-Enein HY (2007). Determination of antioxidant and radical scavenging activity of basil (Ocimum basilicum L. Family Lamiaceae) assayed by different methodologies. Phytotherapy Research 21(4):354-361.
Crossref

  
 

Gupta C, Verma R (2010).Visual estimation and spectrophotometric determination of tannin content and anti-oxidant activity of common vegetable. International Journal of Pharmaceutical Science and Research 2(1):175-182.

  
 

Harborne JB (1998). Phytochemical Methods. A Guide to Modern Technology of Plant Analysis, 3rd Edn. Chapman and Hall, New York pp. 88-185.

  
 

Huang S, Li R, Zhang Z, Li L, Gu X (2009). The genome of the cucumber (Cucumis sativus) L. Nature genetics 41(12):1275-1281
Crossref

  
 

Ibegbulem CO, Ayalogu EO, Uzohu MN (2003). Phytochemical, anti-nutritional contents and hepatotoxicity of zobo (Hibiscus sabdariffa) drink. Journal of Agriculture and Food Science 1(1):335-339.

  
 

Ibraheem O, Maimako RF (2014). Evaluation of alkaloids and cardiac glycosides contents of Ricinus communis Linn (Castor) whole plant parts and determination of their biological properties. International Journal of Toxicological and Pharmacological Research 6(3):34-42.

  
 

Ibrahim ID, Muhammad I, Ashiru S, Sani I, Shehu K, Aliero AA, Aliyu RU (2014). Qualitative and quantitative phytochemical screening of Mimoso pudica plant extracts (touch me not). American Journal of Biological Chemistry 2(2):8-16.

  
 

Iwu MM (2000). Handbook of Africa Medicinal Plants, CRC Press, London P 19.

  
 

James DB, Owolabi OA, Ibrahim AB, Folorunshio DF, Bwalla I, Akanta F (2010). Changes in lipid profile of aqueous and ethanolic extract of Blighiasapide in rats. Asian Journal of Medical Science 2:177-180.

  
 

Jing LJ, Mohamed M, Rahmat A, Bakar MFA (2010). Phytochemicals, antioxidant properties and anticancer investigations of the different parts of several gingers species (Boesenbergia rotunda, Boesenbergia pulchella var attenuata and Boesenbergia armeniaca). Journal of Medicinal Plants Research 4(1):27-32.

  
 

Fischman SL (1997). Oral hygiene products: How far have we come in 6000 years? Periodontology 2000 15:7-14.
Crossref

  
 

Jony M, Roksana A (2012). Phytochemical screening and in-vitro evaluation of reducing power, cytotoxicity and anti-fungal activities of ethanol extract of Cucumis sativus L. International Journal of Pharmaceutical and Biological Archives 3(3):555-560.

  
 

Kumar D, Kumar S, Singh J, Rashlimi N, Vashistha BD, Singh N (2010). Free radical scavenging and analgesic activities of Cucumis sativus L. fruit extract. Journal of Young Pharmacist 2(4):365-368.
Crossref

  
 

Liener IE (1994). Implications of anti-nutritional components in soybean foods. CRC Critical Reviews in Food Science and Nutrition 34: 31-37.
Crossref

  
 

Mattila P, Kumpulairen J (2002). Determination of free and total phenolic acids in plants-derived foods by HPLC with diode-array detection. Journal of Agricultural and Food Chemistry 50:3660-3668.
Crossref

  
 

Mujoriya R, Bodla RB (2012). A study on wheat grass and its nutritional value. Food Science and Quality Management 2:1-8.

  
 

Njagi JM, Gitahi SM, Njagi MM, Mwangi BM, Mworia JK, Juma KK, Aliyu U, Mwonjoria K J, Njoroge WA, Abdirahman Y, Ngugi MP, Njagi NME (2015). Determination of hematological effects of methanolic leaf extract of S. incanum in normal mice. Pharmaceutica Analytica Acta 6(10):24-30.

  
 

Okoye NR (2013). Proximate analysis and protein solubility of four cucurbits found in Nigeria. Pakistan Journal of Nutrition 12(1):20-22.
Crossref

  
 

Okwu DE (2005). Phytochemicals, vitamins and mineral contents of two Nigerian medicinal plants. International Journal of Molecular Medicine and Advanced Science 1(14):372-381.

  
 

Okwu DE, Okwu ME (2004).Chemical composition of Spondiasmombin Linn. plants parts. Journal of Sustainable Agriculture and Environment 6:140-147

  
 

Olorunju AE, Adewale A, Modupe MJ (2012). Anti-inflammatory activity of Russelia equisetiformis schlecht & cham: Identification of its active constituent. Journal of Intercultural Ethnopharmacology 1(1):25-29.
Crossref

  
 

Paolo DM, Michael EM, Helmut S (1991). Anti-oxidation defence system: The role of carotenoids, tocopherol, and thiols. American Journal of Clinical Nutrition 53:194-200.
Crossref

  
 

Pearson D (1976). The Chemical Analysis of Foods. 7th ed. London, Churchill Livingstone pp. 3-4.

  
 

Pietta PG (2000). Flavonoids as antioxidants. Journal of Natural Product 63:1035-1042
Crossref

  
 

Quasheeh M (1937). Quantitative Analysis of Glycoside, Practical Guide. Department of Pharmacognsy, King Staub University pp.1-2.

  
 

Shi J, Arunasala K, Yeung D, Kakuda Y, Mittal G, Jiang Y (2004). Saponin from edible legumes: Chemistry, processing and health benefits. Journal of Medicinal Food 7(1): 67-78.
Crossref

  
 

Singh Gill N, Sood S, Muthraman A, Garg M, Kumar R, Bali M, Dev-Sharma P (2010). Antioxidant, anti-inflammatory and analgesic potential of Cucumis sativus seed extract. Latin American Journal of Pharmacy 29:927-932.

  
 

Sotiroudis G, Melliou E, Sotiroudis TG, Chinou I (2010). Chemical analysis, antioxidant and antimicrobial activity of three Greek cucumber (Cucumis sativus) cultivars. Journal of Food Biochemistry 34:61-78.
Crossref

  
 

Trease GE, Evans WC (2002).Textbook of Pharmacognosy.15th Ed. Saunder Publishers, London pp. 42-393.

  
 

Uematsu Y, Hirata K, Saito K (2000). Spectrophotometric determination of saponin in yucca extract used as food additive. Journal of AOAC International 83(6):1451-1454.

  
 

Vivek KB, Ji-Eun K, Yong-Ha P, Sun CK (2017). In vivo pharmacological effectiveness of heat-treated cucumber (Cucumis sativus L.) juice against CCI4- induced detoxification in a rat model. Indian Journal of Pharmaceutical Education and Research 51(2):280-287.
Crossref

  
 

Wadood A, Ghufran M, Jamal SB, Naeem M, Khan A, Ghaffer R, Asna D (2013). Phytochemical analysis of medicinal plants occurring in local area of Mardan. Biochemistry and Analytical Biochemistry 2(4):1-4
Crossref

  
 

Wang YH, Joobeur T, Dean RA, Staub, JE (2007). Cucurbits-genome mapping and molecular breeding in plants 5. Vegetables 375.

  
 

Weickert MO, Pfeiffer AF (2008). Metabolic effects of dietary fibre consumption and prevention of diabetes. Journal of Nutrition 138(3):439-442.
Crossref

  
 

Wolfe K, Wu X, Liu RH (2003). Antioxidant property of apple peels. Journal of Agricultural Food and Chemistry 51(3):609-614.
Crossref

  
 

Yohanna S (2013). Healthy living for CEOs and VIPs.Cucumber, 2nd Edition.Snaap Press, Enugu pp. 40-41.

  

 




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