Full Length Research Paper
ABSTRACT
The main objective of this study was the characterization of new lactobacilli probiotic strains belonging to lactic acid bacteria (LAB). Eighty-eight strains were isolated from different Algerian camel and mare fermented milks; three of them were pre-selected for their stability, fast growth and resistance to acidity and bile salts. Cell viability was assessed in simulated gastric and intestinal conditions. On the other hand, cell safety was checked by testing their hemolytic capacity. The in vitro tests revealed a good probiotic potential of selected strains. The majority of lactobacilli is resistant to cross-stress and persists beyond 4 h of incubation in contact with simulated gastrointestinal juices; a survival rate of over 80% was observed. All strains showed better lipolytic activity in the presence of natural substrates compared to Tween-80. Lipolysis zones diameters obtained in the presence of butter and olive oil were remarkable (between 20 and 27 mm respectively). Investigation of the cholesterol-lowering and the triglyceride-lowering properties revealed a cholesterol ratio degradation of 54.8% and a triglyceride ratio degradation of 80.3% for Lactobacillus plantarum NSC5C.
Key words: Probiotic, camel and mare fermented milks, cholesterol lowering, triglycerides lowering, Lactobacillus plantarum.
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
CONCLUSION
CONFLICT OF INTEREST
The authors Sabrina AMARA, Halima ZADI-KARAM and Nour-Eddine KARAM declare that they have no conflict of interest.
FUNDING
This work was funded by the Algerian Ministry of Higher Education and Scientific Research (MESRS) and the Directorate General for Scientific Research and Technological Development (DGRSDT).
ETHICAL APPROVAL
This article does not contain any studies with human participants or animals performed by any of the authors.
REFERENCES
Aarti C, Khusro A, Varghese R, Arasu MV, Agastian P, Al-Dhabi N.A, Ilavenil S, Choi KC (2017). In vitro studies on probiotic and antioxidant properties of Lactobacillus brevis strain LAP2 isolated from Hentak, a fermented fish product of North-East India. LWT (86):438-446. |
|
Aarti C, Khusro A, Varghese R, Valan-Arasuc M, Agastiana P, Al-Dhabi NA, Ilavenil S, Choid KC (2018). In vitro investigation on probiotic, anti-Candida, and antibiofilm properties of Lactobacillus pentosus strain LAP1. Archives of Oral Biology (89):99-106. |
|
Abdel Gader AM, Alhaider AA (2016). The unique medicinal properties of camel products: A review of the scientific evidence. Journal of Taibah University Medical Sciences 11(2):98-103. |
|
Akalu N, Assefa F, Dessalegn A (2017). In vitro evaluation of lactic acid bacteria isolated from traditional fermented Shamita and Kocho for their desirable characteristics as probiotics. African Journal of Biotechnology 16(12):594-606. |
|
Akpinar A, Yerlikaya O, Kiliç S (2011). Antimicrobial activity and antibiotic resistance of Lactobacillus delbrueckii ssp. bulgaricus and Streptococcus thermophilusstrains isolated from Turkish homemade yoghurts. African Journal of Microbiology Research 5(6):675-682. |
|
Alagawany M Abd El-Hack ME, Farag MR, Sachan S, Karthik K, Dhama K (2018). The use of probiotics as eco-friendly alternatives for antibiotics in poultry nutrition. Environmental Science and Pollution Research 25(11):10611-10618. |
|
Al-Furaih LY, Ababutain IM, Abd-El-Khalek AB, Abdel-Salam AM (2016). Effect of different microencapsulation materials on stability of Lactobacillus plantarum DSM 20174. . African Journal of Biotechnology 15(24)1207-1216. |
|
Anyika KC, Okaiyeto SO, Saidu SN, Ijale GO (2018). Efficacy of two probiotics in the control of Escherichia coli O157:H7 in experimentally infected lambs. African Journal of Microbiology Research 12(10):243-247. |
|
Bahri F (2014). Isolement et caractérisation des souches de lactobacilles à caractères probiotiques à partir de sellesd'enfants. Thèse de Doctorat (Université Constantine I, Algérie). |
|
Bahri F, Lejeune A, Dubois-Dauphin R, Elmejdoub T, Boulahrouf A, Thonart P (2014). Characterization of Lactobacillus strains isolated from Algerian children faeces for their probiotic properties. African Journal of Microbiology Research 8(3)15:297-303. |
|
Balcázar JL, Rojas-Luna T (2007). Inhibitory activity of probiotic Bacillus subtilis UTM 126 against vibrio species confers protection against vibriosis in juvenile shrimp (Litopenaeusvannamei). Current Microbiology 55:409-412. |
|
Belyagoubi L, Abdelouahid DE (2013). Isolation, identification and antibacterial activity of lactic acid bacteria from traditional Algerian dairy products. Advances in Food Sciences 35(1):84-85. |
|
Bendali F, Kerdouche K, Hamma-Faradji S, Drider D (2017). In vitro and in vivo cholesterol lowering ability of Lactobacillus pentosus KF923750. Beneficial Microbes 8(2):271-280. |
|
Bron PA, Molenaar D, de Vos WM and Kleerebezem M (2006). DNA micro-array-based identification of bile-responsive genes in Lactobacillus plantarum. Journal of Applied Microbiology 100(4):728-738. |
|
Bruno FA, Shah NP (2002). Inhibition of pathogenic and putrefactive microorganisms by Bifidobacterium sp. Milchwissenschaft 57(12):617-621. |
|
Charlier CM, Cretenet S, Even Y, Le Loir Y (2009). Interactions between Staphylococcus aureus and lactic acid bacteria: An old story with new perspectives. International Journal of Food Microbiology 131:30-39. |
|
Chaves BD, Brashears MM, Nightingale KK (2017). Applications and safety considerations of Lactobacillus salivarius as a probiotic in animal and human health. Journal of Applied Microbiology 123(1):18-28. |
|
Azcárate-Peril M.A., Raya R.R. (2001). Methods for Plasmid and Genomic DNA Isolation from Lactobacilli. In: Spencer JFT, de Ragout Spencer AL (eds) Food Microbiology Protocols. Methods in Biotechnology (14) Humana Press. |
|
Collado MC, Gueimonde M, Sanz Y, Salminen S (2006). Adhesion properties and competitive pathogen exclusion ability of bifidobacteria with acquired acid resistance. Journal of Food Protection 69(7):1675-1679. |
|
Conway PL, Gorbach SL, Goldin BR (1987). Survival of Lactic Acid Bacteria in the Human Stomach and Adhesion to Intestinal Cells. Journal of Dairy Science 70(1):1-12. |
|
Cotter PD, Hill C (2003). Surviving the acid test: responses of Gram positive bacteria to low pH. Microbiology and Molecular Biology Reviews 67:429-453. |
|
De Moraes J, Chandan RC (1982). Factors influencing the production and activity of a Streptococcus thermophilus lipase. Journal of Food Science 47:1579-1583. |
|
De Smet I, Van Hoorde L, Vande Woestyne M, Christiaens H, Verstraete W (1995). Significance of bile salt hydrolytic activities of lactobacilli. Journal of Applied Microbiology 79(3):292-301. |
|
Deng Y, Ryu JH, Beuchat LR (1999). Tolerance of acid-adapted and non-adapted E. coli O157: H7 cells to reduced pH as affected by type of acidulant. Journal of Applied Microbiology 86:203-210. |
|
Desmazeaud M (1996). Les bactéries lactiques dans l'alimentation humaine: Utilisation et innocuité. Cahiers Agricultures 5:331-343. |
|
Di Cagno R, De Angelis M, Limitone A, Fox PF, Gobbetti M (2006a). Response of Lactobacillus helveticus PR4 to heat stress during propagation in cheese whey with a gradient of decreasing temperatures. Applied and Environmental Microbiology 72:4503-4514. |
|
Di Cagno R, De Angelis M, Limitone A, Minervini F, Carnevali P, Corsetti A, Gaenzle M, Ciati R, Gobbetti M (2006b). Glucan and fructan production by sourdough Weissellacibaria and Lactobacillus plantarum. Journal of Agricultural and Food Chemistry 54(26):9873-9881. |
|
Digo CA, Kamau-Mbuthia E, Matofari JW, Ng'etich WK (2017). Potential probiotics from traditional fermented milk, Mursik of Kenya. International Journal of Nutrition and Metabolism 10(9):75-81. |
|
Dinçer E, Kıvanç M (2018). Lipolytic Activity of Lactic Acid Bacteria Isolated from Turkish Pastırma. Anadolu Üniversitesi Bilimve Teknoloji Dergisi - C YaÅŸam Bilimleri Ve Biyoteknoloji 7(1):12-19. |
|
Ding W, Shia C, Chen M, Zhou J, Long R and Guo X (2017). Screening for lactic acid bacteria in traditional fermented Tibetan yak milk and evaluating their probiotic and cholesterol-lowering potentials in rats fed a high-cholesterol diet. Journal of Functional Foods 32:324-332. |
|
Even S, Lindley ND, Loubiere P, Cocaign-Bousquet M (2002). Dynamic response of catabolic pathways to autoacidification in Lc. lactis: transcript profiling and stability in relation to metabolic and energetic constraints. Applied and Environmental Microbiology 45:1143-1152. |
|
Fang SB, Lee HC, Hu JJ, Hou SY, Liu HL, Fang HW (2009). Dose-dependent effect of Lactobacillus rhamnosuson quantitative reduction of faecal rotavirus shedding in children. Journal of Tropical Pediatrics 55(5):297-301. |
|
Fernandez A, Ogawa J, Penaud S, Boudebbouze S, Ehrlich D, van de Guchte M, Maguin E (2008). Rerouting of pyruvate metabolism during acid adaptation in Lactobacillus bulgaricus. Proteomics 8:3154-3163. |
|
Ferrières J, Dauchet L, Arveiler D, Yarnell JW, Gey F, Ducimetière P, Ruidavets JB, Haas B, Evans A, Bingham A, Amouyel P, Dallongeville J (2004). Frequency of fruit and vegetable consumption and coronary heart disease in France and Northern Ireland: the PRIME study. British Journal of Nutrition 92(6):963-972. |
|
Fleming HP, Etchells JL and Costilow RN (1975). Microbiological inhibition of isolate of Pediococcus from cucumber brine. Applied and Environmental Microbiology 30:1040-1042. |
|
Fooks LJ, Gibson GR (2002). Probiotics as modulators of the gut flora. British Journal of Nutrition 88:S39-S49. |
|
Gao Y, Li D (2018). Screening of lactic acid bacteria with cholesterol-lowering and triglyceride-lowering activity in vitro and evaluation of probiotic function. Annals of Microbiology 68(9):537-545. |
|
García-Cayuela T, Tabasco R, Peláez C, Requena T (2009). Simultaneous detection and enumeration of viable lactic acid bacteria and bifidobacteria in fermented milk by using propidium monoazide and real-time PCR. International Dairy Journal (19):405-409. |
|
Gonzalez-Cuello RE, Colpas-Castillo F, Tarón-Dunoyer F (2017). Protection of Lactobacillus acidophilus under in vitro gastrointestinal conditions employing binary microcapsules containing inulin. African Journal of Biotechnology 16(3):132-138 |
|
Guiraud J, Galzy P (1980). L'analyse microbiologique dans les industries alimentaires. Edition l'usine 119 p. |
|
Guo LD, Yang LJ, Huo GC (2011). Cholesterol Removal by Lactobacillus plantarum Isolated from Homemade Fermented Cream in Inner Mongolia of China. Czech Journal of Food Sciences 29(3):219-225 |
|
Heunis T, Deane S, Smit S, Dicks LM (2014). Proteomic profiling of the acid stress response in Lactobacillus plantarum 423. Journal of Proteome Research 13:4028-4039. |
|
Huang Y, Wang X, Wang J, Wu F, Sui Y, Yang L and Wang Z (2013). Lactobacillus plantarum strains as potential probiotic cultures with cholesterol-lowering activity. Journal of Diary Science 96(5):2746-2753. |
|
Idoui T (2008). Bactérie lactiques indigènes : Isolement, identification et propriétés technologiques : Effets probiotiques chez le poulet de chair ISA15, le lapin de souche locale et le rat Wistar, Université Ahmed Ben Bella d'Oran1 Es Senia. |
|
Isolauri E, Salminen S, Ouwehand A (2004). Microbial-gut interactions in health and disease, Probiotics. Best Practice and Research Clinical Gastroenterology 18(2):299-313. |
|
Izquierdo E, Marchioni E, Aoude-Werner D, Hasselmann C, Ennahar S (2009). Smearing of soft cheese with Enterococcus faecium WHE 81, a multi-bacteriocin producer, against Listeria monocytogenes. Food Microbiology 26:16-20. |
|
Jaspers DA, Massey LK, Leudecke LO (1984). Effect of consuming yogurts prepared with three culture strains on human serum lipoproteins. Journal of Food Science 49:1178-1181. |
|
Jastrzębska W, Wadas E, Daszkiewicz T, Pietrzak-Fiećko R (2017). Nutritional Value and Health-Promoting Properties of Mare's Milk - a Review. Czech Journal of Animal Science 62(12):511-518. |
|
Kamal AM, Salama OA (2009). Lipid fractions and fatty acid composition of colostrums, transitional and mature she-camel milk during the first month of lactation Asian. The American Journal of Clinical Nutrition 1:23-30. |
|
Kamaly KM, El Soda M, Marth EH (1988). Esterolytic activity of Streptococcus lactis,Streptococcus cremoris and their mutants. Milchwissenschaft 43:346-349. |
|
Katz M, Sarvary I, Frejd T, Hahn-Hägerdal B, Gorwa-Grauslund MF (2002). An improved stereoselective reduction of a bicyclic diketone by Saccharomyces cerevisiae combining process optimization and strain engineering. Applied Microbiology and Biotechnology 59(6):641-648. |
|
Kondo S, Xiao JZ, Satoh T, Odamaki T, Takahashi S, Sugahara H et al. (2010). Antiobesity effects of Bifidobacterium breve strain B-3 supplementation in a mouse model with high-fat diet-induced obesity. Bioscience, Biotechnology, and Biochemistry 74:1656-1661. |
|
Konuspayeva G, Lemarie E, Faye B, Loiseau G, Montet D (2008). Fatty acid and cholesterol composition of camel's (Camelusbactrianus, Camelusdromedariusand hybrids) milk in Kazakhstan. Dairy Science and Technology 88(3):327-340. |
|
Koponen J, Laakso K, Koskenniemi K, Kankainen M, Savijoki K, Nyman TA, de Vos WM, Tynkkynen S, Kalkkinen N and Varmanen P (2012). Effect of acid stress on protein expression and phosphorylation in Lb. rhamnosus GG. Journal of Proteomics 75:1357-1374. |
|
Lane DJ (1991). 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic Acids Techniques in Bacterial Systematics.Wiley, Chichester pp. 115-147. |
|
Liao SF, Nyachoti M (2017). Using probiotics to improve swine gut health and nutrient utilization. Animal Nutrition 3(4):331-343. |
|
Lilly DM, Stillwell RH (1965). Probiotics: growth-promoting factors produced by microorganisms. Science 147 (3659):747-748. |
|
Lin WH, Yu B, Jang SH, Tsen HY (2007). Different probiotic properties for Lactobacillus fermentum strains isolated from swine and poultry. Anaerobe 13(3-4):107-113. |
|
Lindwall S and Fonden R (1984). Passage and survival of L. acidophilus in the human gastrointestinal tract. International Dairy Federation Bulletin 21:179. |
|
Liu DM, Guo J, Zeng XA, Sun DW, Brennan CS, Zhou QX, Zhou JS (2016). The probiotic role of Lactobacillus plantarum in reducing risks associated with cardiovascular disease. International Journal of Food Science and Technology 52(1):127-136. |
|
Mahdhi A, Harbi B, Ángeles Esteban M, Chaieb K, Kamoun F, Bakhrouf A (2010). Using mixture design to construct consortia of potential probiotic Bacillus strains to protect gnotobiotic Artemia against pathogenic Vibrio. Biocontrol Science and Technology 20:983-996. |
|
Manson JE, Tosteson H, Ridker PM, Satterfield S, Hebert P, O'Connor GT, Buring JE, Hennekens CH (1992). The primary prevention of myocardial infraction, The New England Journal of Medicine 326:1406-1416. |
|
Mansoub NH (2010). Effect of Probiotic Bacteria Utilization on Serum Cholesterol and Triglycerides Contents and Performance of Broiler Chickens. Global Veterinaria 5 (3):184-186. |
|
Maragkoudakis E, Realdi G, Dore MP (2005). Fungal infections of the gastrointestinal tract. Recenti Progressi in Medicina 96(6):311-317. |
|
Maragkoudakis PA, Zoumpopoulou G, Miaris C, Kalantzopoulos G, Pot B, Tsakalidou T (2006). Probiotic potential of Lactobacillus strains isolated from dairy products. International Dairy Journal 16(3):189-199. |
|
Marceau A, Zagorec M, Champomier-Vergès MC (2002). Analysis of Lactobacillus sakei adaptation to its environment by a proteomic approach. Sciences des Aliments 22:97-105. |
|
Marteau P, Shanahan MD (2003). Basic aspects and pharmacology of probiotics: an overview of pharmacokinetics, mechanisms of action and side-effects. Best Practice and Research Clinical Gastroenterology 17(5):725-740. |
|
Mathipa MG, Thantsha MS (2015). Cocktails of probiotics pre-adapted to multiple stress factors are more robust under simulated gastrointestinal conditions than their parental counter parts and exhibit enhanced antagonistic capabilities against Escherichia coli and Staphylococcus aureus. Gut Pathogens 7:5. |
|
Merzoug M, Dalache F, Zadi Karam H, Karam NE (2016). Isolation and preliminary characterisation of bacteriocin,produced by Enterococcus faecium GHB21 isolated from Algerian paste of dates "ghars". Annals of Microbiology 66(2):795-805. |
|
Merzoug M, Mosbahi K, Walker D, Karam NE (2018). Screening of the Enterocin-Encoding Genes and Their Genetic Determinism in the Bacteriocinogenic Enterococcus faecium GHB21. Probiotics and Antimicrobial Proteins 10:1007. |
|
Mirlohi M, Soleimanian-Zad S, Dokhani S, Sheikh-Zeinodin M, Abghary A (2009). Investigation of Acid and Bile Tolerance of Native Lactobacilli Isolated from Fecal Samples and Commercial Probiotics by Growth and Survival Studies. Iranian Journal of Biotechnology (7)4:233-240. |
|
Okpara AN, Okolo BN, Ugwuanyi JO (2014). Antimicrobial activities of lactic acid bacteria isolated from akamu and kunun-zaki (cereal based non-alcoholic beverages) in Nigeria. African Journal of Biotechnology 13(29)2977-2984. |
|
Olek A, Woynarowski M, Ahrén IL, Kierkus J, Socha P, Larsson N, Önning G (2017). Efficacy and safety of Lactobacillus plantarum DSM 9843 (LP299V) in the prevention of antibiotic associated gastrointestinal symptoms in children randomized, double-blind placebo-controlled study. Journal of Pediatrics 186:82-86. |
|
Papon M, Talon R (1989). Cell location and partial characterization of Brochothrixthermosphacta and Lactobacillus curvatus lipases. Journal of Applied Microbiology 66:235-242 |
|
Pereira DI, McCartney AL, Gibson GR (2003). An in vitro study of the probiotic potential of a bile-salt-hydrolyzing Lactobacillus fermentum strain, and determination of its cholesterol-lowering properties. Applied and Environmental Microbiology 69(8):4743-4752. |
|
Pieszka M, Luszczynski J, Zamachowska M, Augustyn R, Dlugosz B, Hedrzak M (2016). Is mare milk an appropriate food for people? - A review. Annals of Animal Science 16:33-51. |
|
Raziq A, Younas M, Kakar MA (2008). Camel-a potential diary animal in difficult environments. Pakistan Journal of Agricultural Sciences 45(2):263-267. |
|
Reyes-Nava LA, Garduño-Siciliano L, Estrada-de los Santos P, Hernández-Sánchez HA, Arauz J, Muriel P, Rivera Espinoza Y (2016). Use of bile acids as a selection strategy for lactobacillus strains with probiotic potential. Journal of Food and Nutritional Disorders 5:1. |
|
Rudel LL, Felts JM, Morris MD (1973). Exogenous cholesterol transport in rabbit plasma lipoproteins. Biochemical Journal 134(2):531-537. |
|
Rychen G, Aquilina G, Azimonti G, Bampidis V,De Lourdes Bastos M, Bories G, Chesson A et al. (2017). Safety and efficacy of Lactobacillus buchneri NRRL B-50733 as a silage additive for all animal species. EFSA Journal 15(7):04934-04939. |
|
Saarela M, Rantala M, Hallamaa K, Nohynek L, Virkajarvi I and Matto J (2004). Stationary-phase acid and heat treatments for improvement of the viability of probiotic lactobacilli and bifidobacteria. Journal of Applied Microbiology 96:1205-1214. |
|
Salminen S, Bouley MC, Boutron-Rualt MC, Cummings J, Franck A, Gibson G, Isolauri E, Moreau MC, Roberfroid M and Rowland I (1998). Functional Food Science and Gastrointestinal Physiology and Function. British Journal of Nutrition 1:147-171. |
|
Sanchez T, Lamela L, Valdes R and Lopez O (2006). Evaluation of the productive indicators of Holstein cows in pedestals. Pastos y Forrajes 29(1):51-60. |
|
Savadogo A, Cheik AT, Ouattara imael HN, Bassole A, Traore S (2004). Antimicrobial activities of lactic acid bacteria strains isolated from Burkina Faso fermented milk. Pakistan journal of nutrition 3(3):174-179. |
|
Servin AL (2004). Antagonistic activities of lactobacilli and bifidobacteria against microbial pathogens. FEMS Microbiological Reviews 28(4):405-440. |
|
Shah NP (2007). Functional cultures and health benefits. International Dairy Journal 17(11):262-1277. |
|
Shahab-Lavasani S, Ehsani M, Mirdamadi S and Mousavi S (2012). Study of the proteolysis and lipolysis of probiotic Lighvan cheese. International Journal of Agricultural Research 2:341-352. |
|
Siegumfeldt H, Rechinger KB, Jakobsen M (2000). Dynamic changes of intracellular pH in individual lactic acid bacterium cells in response to a rapid drop in extracellular pH. Applied and Environmental Microbiology 66:2330-2335. |
|
Soomro RM, Bucur IJ and Noorani S (2002). Cumulative incidence of venous thromboembolism during pregnancy and puerperium: a hospital-based study. Angiology 53(4):429-34. |
|
Tadesse G, Ephraim E, Ashenafi M (2004). Assessment of the antimicrobial activity of lactic acid bacteria isolated from Borde and Shamita, traditional Ethiopian fermented beverages, on some foodborne pathogens and effect of growth medium on the inhibitory activity. The International Journal of Food Safety 5:13-20. |
|
Tashakor A, zadehdehkordi MH, Emruzi Z, Gholami D (2017). Isolation and Identification of a Novel Bacterium, Lactobacillus Sakei Subsp. Dgh Strain 5, and Optimization of Growth Condition for Highest Antagonistic Activity. Microbial Pathogenesis 106:78-84. |
|
Tejero-Sariñena S, Barlow J, Costabile A, Gibson GR and Rowland I (2012). In vitro evaluation of the antimicrobial activity of a range of probiotics against pathogens: evidence for the effects of organic acids. Anaerobe 18(5):530-538. |
|
Tremonte P, Sorrentino E, Pannella G, Tipaldi G, Sturchio M, Masucci A, Maiuro L, Coppola R. and Succi M (2017). Detection of different microenvironments and Lactobacillus sakei biotypes in Ventricina, a traditional fermented sausage from central Italy. International Journal of Food Microbiology 242:132-140. |
|
Vaughan EE, Beat Mollet B and DeVos WM (1999). Functionality of probiotics and intestinal lactobacilli: light in the intestinal tract tunnel. Current Opinion in Microbiology 10:505-510. |
|
Wu R, Sun Z, Wu J, Meng H, Zhang H (2010). Effect of bile salts stress on protein synthesis of Lactobacillus casei Zhang revealed by 2-dimensional gel electrophoresis. Journal of Dairy Science 93(8):3858-68. |
|
Zhai Z, Douillard FP, An H, Wang G, Guo X, Luo Y, Hao Y (2014). Proteomic characterization of the acid tolerance response in Lactobacillus delbrueckii subsp. Bulgaricus CAUH1and functional identification of a novel acid stress-related transcriptional regulator Ldb0677. Environmental Microbiology 16:1524-1537. |
|
Zhang F, Qiu L, Xu X, Liu Z, Zhan H, Tao X, Shah N, Wei H (2017). Beneï¬cial effects of probiotic cholesterol-lowering strain of Enterococcus faecium WEFA23 from infants on diet-induced metabolic syndrome in rats. Journal of Diary Science 100(3):1618-1628. |
|
Zhang M, Hang X, Fan X, Li D, Yang H (2008). Characterization and selection of Lactobacillus strains for their effect on bile tolerance, taurocholate deconjugation and cholesterol removal. World Journal of Microbiology Biotechnology 4(1):7-14. |
|
Zuljan FA, Repizo GD, Alarcón SH, Magni C (2014). Acetolactate synthase of Lactococcus lactis contributes to pH homeostasis in acid stress conditions. International Journal of Food Microbiology 188:99-107. |
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