African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Milk clotting and proteolytic activity of enzyme preparation from Pediococcus acidilactici SH for dairy products

Mohamed Muftah Imdakim
  • Mohamed Muftah Imdakim
  • Faculty of Science and Technology, Universiti Sains Islam Malaysia, Bandar Baru Nilai, 71800 Nilai, Negeri Sembilan, Malaysia.
  • Google Scholar
Zaiton Hassan*
  • Zaiton Hassan*
  • Faculty of Science and Technology, Universiti Sains Islam Malaysia, Bandar Baru Nilai, 71800 Nilai, Negeri Sembilan, Malaysia.
  • Google Scholar
Mohamed Mustafa Aween
  • Mohamed Mustafa Aween
  • Faculty of Science and Technology, Universiti Sains Islam Malaysia, Bandar Baru Nilai, 71800 Nilai, Negeri Sembilan, Malaysia.
  • Google Scholar
Brahim Mustafa Elshaafi
  • Brahim Mustafa Elshaafi
  • Faculty of Science and Technology, Universiti Sains Islam Malaysia, Bandar Baru Nilai, 71800 Nilai, Negeri Sembilan, Malaysia.
  • Google Scholar
Belal Jamal Muhialdin
  • Belal Jamal Muhialdin
  • Faculty of Food Science and Technology, Universiti Putra Malaysia (UPM), UPM Serdang, 43400, Selangor, Malaysia.
  • Google Scholar


  •  Received: 10 June 2014
  •  Accepted: 15 August 2014
  •  Published: 14 January 2015

References

Arima KYUJ, Iwasaki S, Tamura G (1968). Milk-clotting enzyme from microorganisms: V. Purification and crystallization of Mucor rennin from Mucor pusillus var. lindt. Appl. Environ. Microb. 16:1727-173.

 

Aween MM, Hassan Z, Muhialdin BJ, Noor HM, Eljamel YA (2012). Evaluation on antibacterial activity of Lactobacillus acidophilus strains isolated from honey. Am. J. Appl. Sci. 6:807-817.
Crossref

 
 

Bradford MM (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein dye binding. Anal. Biochem. 72:248-254.
Crossref

 
 

Cavalcanti MTH, Martinez CR, Furtado VC, Neto BB, Teixera MF, Lima FJL, Porto ALF (2005). Milk-clotting protease production by Nocardiopsis sp. in an inexpensive medium. World J. Microb. Biotechnol. 21:151-154.
Crossref

 
 

Chazarra S, Sidrach L, López-Molina D, Rodríguez-López JN (2010). Characterization of the milk-clotting properties of extracts from artichoke (Cynara scolymus, L.) flowers. Int. Dairy J. 17:1393-1400.
Crossref

 
 

Cheng K, Lu F, Li M, Liang X (2010). Purification and biochemical characterization of a serine alkaline protease TC4 from a new isolated Bacillus alcalophilus TCCC11004 in detergent formulations. Afr. J. Biotechnol. 9:4942-4953.

 
 

Chu IM, Lee C, Li TS (1992). Production and degradation of alkaline protease in batch cultures of Bacillus subtilis ATCC 14416. Enzyme Microb. Technol. 14:755-761.
Crossref

 
 

D'souza TM, Pereira L (1982). Production and immobilization of a bacterial milk-clotting enzyme. J. Dairy Sci. 65:2074-2081.
Crossref

 
 

De-Lima CJB, Mariana C, Lovaglio RB, Ribeiro EJ, Contiero J, De-Araújo EH (2008). Production of rennet in submerged fermentation with the filamentous fungus Mucor miehei NRRL 3420. World. Appl. Sci. J. 4:578-585.

 
 

Dutt K, Meghwanshi GK, Gupta P, Saxena RK (2008). Role of casein on induction and enhancement of production of a bacterial milk clotting protease from an indigenously isolated Bacillus subtilis. Lett. Appl. Microb. 46:513-518.
Crossref

 
 

Egito AS, Girardetc JM, Lagunaa LE, Poirsonc C, Molle D’Micloc bL, Humbertc G, Gaillard JL (2007). Milk-clotting activity of enzyme extracts from sunflower and albizia seeds and specific hydrolysis of bovine k-casein. Int. Dairy J. 17:816-825.
Crossref

 
 

El-Bendary MA, Moharam ME, Ali TH (2007). Purification and characterization of milk-clotting enzyme produced by Bacillus sphaericus. J. Appl. Sci. 3:695-699.

 
 

El-Sayed ST, Elmazar MMA, Al-Azzouny RA (2013). Purification and Characterization of a Novel Milk Clotting Enzyme from Brassica napus Seeds. Aus. J. Basic. Appl. Sci. 7:482-493.

 
 

He X, Ren F, Guo H, Zhang W, Song Xi, Gan B (2011). Purification and properties of a milk-clotting enzyme producedby Bacillus amyloliquefaciens D4. Kor. J. Chem. Eng. 28:203-208.
Crossref

 
 

Hebert EM, De Giori GS, Raya RR (2001). Isolation and Characterization of a Slowly Milk-Coagulating Variant of Lactobacillus helveticus. Appl. Environ. Microb. 67:1846-1850.
Crossref

 
 

Irigoyen A, Izco JM, IbaÂnÄez FC, Torre P (2001). Influence of rennet milk-clotting activity on the proteolytic and sensory characteristics of an ovine cheese. Food Chem. 72:137-144.
Crossref

 
 

Isma AM, Isao M, Elfadil EB (2009). Characterisation of partially milk-clotting enzyme from Solanum dubium fresen seeds. Food. Chem. 116:395-400.
Crossref

 
 

Kathiresan K, Manivannan S (2007). Production of alkaline protease by Streptomyces sp. isolated from coastal mangrove sediment. Res. J. Environ. Sci. 1:173-178.
Crossref

 
 

Keila AM, Maria TH, Helena SD, Elias BT, Eduardo HM, Lins AL, Figueiredo P (2001). Partial characterization of proteases from Streptomyce clavuligerus using an inexpensive medium. Braz. J. Microb. 32:76-82.

 
 

Khademi F, Abachi S, Mortazavi A, Ehsani MA, Tabatabaei MR, Malekzadeh FA (2013). Optimization of fungal rennet production by local isolate of Rhizomucor nainitalensis under solid substrate fermentation system. IOSR J. Pharm. Biol. Sci. 5:115-121.

 
 

Laemmli UK (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
Crossref

 
 

Lee CY, Cheng MF, Yu MS, Pan MS (2002). Purification and characterization of a putative virulence factor, serine protease, from Vibrio parahaemlyticus. FEMS Microb. Letts. 209:31-37.
Crossref

 
 

Merheb CW, Cabral H, Gomes E, Da-Silva R (2007). Partial characterization ofprotease from thermophilic fungus Thermoascus aurantiacus and its hydrolytic activity on bovine casein. Food Chem. 104:127-131.
Crossref

 
 

Merheb-Dini C, Gomes E, Boscolo M, da Silva R (2010). Production and characterization of a milk-clotting protease in the crude enzymatic extract from the newly isolated Thermomucor indicae-seudaticae N31: (Milk-clotting protease from the newly isolated Thermomucor indicae-seudaticae N31). Food Chem. 120:87-93.
Crossref

 
 

Moon SH, Parulekar SJ (1991). A parametric study ot protease production in batch and fedbatch cultures of Bacillus firmus. Biotechnol. Bioeng. 37:467-483.
Crossref

 
 

Moosavi-Nasab M, Radi M, Jouybari HA (2010). Investigation of enzyme modified cheese production by two species of Aspergillus. Afr. J. Biotechnol. 9:508-511.

 
 

Patel R, Dodia M, Singh SP (2005). Extracellular alkaline protease from a newly isolated haloalkaliphilic Bacillus sp.: Production and optimization. Process Biochem. 40:3569-3573
Crossref

 
 

Poza M, Sieiro C, Carreira L, Barros-Velazquez J, Villa TG (2003). Production and characterization of the milk-clotting protease of Myxococcus xanthus strain 422. J. Ind. Microb. Biotechnol. 30:691-698.
Crossref

 
 

Reps A, Jêdrychowski L, Wisniewska K, Jankowska A (2006). Application of a coagulating preparation obtained with Rhizomucor miehei N in cheese-making. Pak. J. Nutr. 5:97-101.
Crossref

 
 

Richardson GH, Nelson JH, Lubnow RE (1967). Rennin like enzyme from Mucor pusillus for cheese manufacture. J. Dairy Sci. 50:1066-1072.
Crossref

 
 

Rifaat HM, Hassanein SM, El-Said OH, Saleh SA, Selim MSM (2005). Purification and characterisation of extracellular neutral protease from Streptomyces microflavus. Arab. J. Biotechnol. 9:51-60575.

 
 

Roseiro LB, Barbosa M, Ames JM, Wilbey A (2003). Cheesemaking with vegetable coagulants-The use of Cynara L. for the production of ovine milk cheese. Int. J. Dairy Technol. 56:76-85.
Crossref

 
 

Sandra S, Ho M, Alexander M, Corredig M (2012). Effect of soluble calcium on the renneting properties of casein micelles as measured by rheology and diffusing wave spectroscopy. J. Dairy Sci. 95:75-82.
Crossref

 
 

Sato S, Tokuda H, Koizumi T, Nakanishi K (2004). Purification and Characterization of an Extracellular Proteinase Having Milk-Clotting Activity from Enterococcus faecalis TUA2495L. Food Sci. Technol. Res. 10:44-50.
Crossref

 
 

Shata HMA (2005). Extraction of milk-clotting enzyme produced by solid state fermentation of Aspergillus oryzae. Pol. J. Microb. 54:241-247.
Pubmed

 
 

Shieh CJ, Phan-Thi LA, Shih IL (2009). Milk clotting enzymes produced by culture of Bacillus subtilis natto. Biochem. Eng. J. 43:85-91.
Crossref

 
 

Silveira GG, Oliveira GM, Ribeiro EJ, Monti R, Contiero J (2005). Microbial rennet produced by Mucor miehei in solid state and submerged fermentation. Braz. Arch. Biol. Technol. 6:931-937.
Crossref

 
 

Srinivasan M, Dhar SC (1990). Factors influencing extracellular protease synthesis in an Aspergillus flavus isolate. Acta. Microb. Hung. 37:15-23.
Pubmed

 
 

Vairo-Cavalli S, Claver S, Priolo N, Natalucci C (2005). Extraction and partial characterization of a coagulant preparation from Silybum marianum flowers. Its action on bovine caseinate. J. Dairy Res. 72:271-275.
Crossref

 
 

Vishwanatha KS, Rao AAG, Singh SA (2010). Production and characterization of a milk-clotting enzyme from Aspergillus oryzae MTCC 5341. Appl. Microb. Biotechnol. 85:1849-1859.
Crossref

 
 

Wu FC, Chang CW, Shih IL (2013). Optimization of the production and characterization of milk clotting enzymes by Bacillus subtilis natto. SpringerPlus 2:33.
Crossref

 
 

Xing G, Pan D, Tong M, Zeng X (2012). Purification and characterization of cell-envelopeproteinase from Lactobacillus casei DI-1. Afr. J. Biotechnol. 11:15060-15067.

 
 

Yegin S, Fernández-Lahore M, Guvenc U, Goksungur Y (2010). Production of extracellular aspartic protease in submerged fermentation with Mucor mucedo DSM 809. Afr. J. Biotechnol. 38:6380-6386.

Academic Journals

 
 

Yu PJ, Chou CC (2005). Factors affecting the growth and production of milk-clotting by Amylomyces rouxii in rice liquid medium. Food Technol. Biotechnol. 43:283-288.