African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12421

Full Length Research Paper

Genomic sequencing and recombinant expression of proinsulin isolated from cow and buffalo in Pakistan

Farheen Aslam
  • Farheen Aslam
  • Department of Biotechnology, Lahore College for Women University, Lahore, Pakistan.
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Hooria Younas
  • Hooria Younas
  • School of Biological Sciences, University of the Punjab, Lahore, Pakistan.
  • Google Scholar
Saima Iftikhar Bajwa
  • Saima Iftikhar Bajwa
  • School of Biological Sciences, University of the Punjab, Lahore, Pakistan.
  • Google Scholar

  •  Received: 08 April 2019
  •  Accepted: 16 May 2019
  •  Published: 31 July 2019


Abdel-Rahman S (2006). Evidences reveal that cattle and buffalo evolutionary derived from the same ancestor based on cytogenetic and molecular markers. Biotechnology in Animal Husbandry 22(3-4):1-9.


Aslam F, Gardner Q-tAA, Zain H, Nadeem MS, Ali M, Rashid N, Akhtar M (2013). Studies on the expression and processing of human proinsulin derivatives encoded by different DNA constructs. Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics 1834(10):2116-23.


Bimboim H, Doly J (1979). A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic acids Research 7(6):1513-23.


Bolivar F (1994). Production in Escherichia coli of a rat chimeric proinsulin polypeptide carrying human A and B chains and its preparative chromatography. Journal of biotechnology 38(1):89-96.


Chan SJ, Keim P, Steiner DF (1976). Cell-free synthesis of rat preproinsulins: characterization and partial amino acid sequence determination. Proceedings of the National Academy of Sciences 73(6):1964-8.


Chomczynski P, Sacchi N (1987). Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical biochemistry 162(1):156-159.


Olmos J, Cruz N, Sánchez M, López M, Balbás P, Gosset G, Valle F, Patzelt C, Labrecque AD, Duguid JR, Carroll RJ, Keim PS, Heinrikson RL, Steiner DF (1978). Detection and kinetic behavior of preproinsulin in pancreatic islets. Proceedings of the National Academy of Sciences 75(3):1260-1264.


Sambrook J, Russell D (2001). Molecular Cloning: A Laboratory Manual. 3rd Ed New York: Cold Spring Harbor Laboratory Press.


Younas H (2009). Studies on recombinant buffalo (Bubalus bubalis) proinsulin and its derivatives (Doctoral dissertation, University of Punjab, Lahore).