African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Full Length Research Paper

Purification and characterization of phenoloxidase from immunized haemolymph of Schistocerca gregaria

Mahmoud, D. M.
  • Mahmoud, D. M.
  • Department of Entomology, Faculty of Science, Ain Shams University, Abbassya, Cairo, Egypt.
  • Google Scholar
Salem, D. A. M.
  • Salem, D. A. M.
  • Department of Entomology, Faculty of Science, Ain Shams University, Abbassya, Cairo, Egypt.
  • Google Scholar
Mo'men, S. A.
  • Mo'men, S. A.
  • Department of Entomology, Faculty of Science, Ain Shams University, Abbassya, Cairo, Egypt.
  • Google Scholar
Barakat, E. M. S.
  • Barakat, E. M. S.
  • Department of Entomology, Faculty of Science, Ain Shams University, Abbassya, Cairo, Egypt.
  • Google Scholar
Salama, M. S.
  • Salama, M. S.
  • Department of Entomology, Faculty of Science, Ain Shams University, Abbassya, Cairo, Egypt.
  • Google Scholar


  •  Received: 19 May 2015
  •  Accepted: 11 September 2015
  •  Published: 04 November 2015

References

Ajamhassani M, Sendi JJ, Farsi MJ, Zibaee A (2012). Purification and characterization of phenoloxidase from the hemolymph of Hyphantria cunea (Lepidoptera: Arctiidae). Department of Plant Protection, College of Agriculture, University of Guilan-Rasht, 41635â1314, Iran.
 
Andersen SO (1980). Cuticular Sclerotization. In: Miller, TA (eds.), Cuticle techniques in Arthropods. Springer Verlag, New York. pp. 185-215.
Crossref
 
Andersson K, Sun SC, Boman HG, Steiner H (1989). Purification of the prophenoloxidase from Hyalophora cecropia and four proteins involved in its activation. Insect Biochem. 19:629 - 637.
Crossref
 
Asano T, Ashida M (2001). Cuticular pro-phenoloxidase of silkworm, Bombyx mori. J. Biol. Chem. 276:11100-11112.
Crossref
 
Ashida M, Ishizaki Y, Iwahana H (1983). Activation of prophenoloxidase by bacterial cell walls or beta-1, 3-glucans in plasma of the silkworm, Bombyx mori. Biochem. Biophys. Res. Community 113: 562 - 568.
Crossref
 
Aso Y, Karmer K, Hopkins T, Lookhart GL (1985). Characterization of haemolymph protyrosinase and cuticular activator from Manduca sexta. Insect Biochem. 15: 9 - 17.
Crossref
 
Aspan A, Söderhäll K (1991). Purification of prophenoloxidase from crayfish cells, and its activation by an endogenous serine proteinase. Insect Biochem. 21: 363 - 373.
Crossref
 
Aspan A, Huang TS, Cerenius L, Sِderhall K (1995). cDNA cloning of prophenoloxidase from the freshwater crayfish Pacifartacus leniuscufus and its activation. Proc. Natl. Acad. Sci. USA 92:939 - 943.
 
Barakat EMS. (1997). A comparative study on the immune response of the wax moth, Galleria mellonella (L.) to some biotic and abiotic materials. Ph. D Thesis. Ain Shams University.
 
Barakat EMS, Meshrif WS, Shehata MG (2002). Changes in the haemolymph of the desert locust, Schistocerca gregaria after injection of Bacillus thuringiensis. J. Egypt. Acad. Soc. Environ. Dev. 2: 95 - 115.
 
Barrett FM (1987). Phenoloxidases from larval cuticle of the sheep blowfly, Lucilia cuprina: characterization developmental changes and inhibition by antiphenoloxidase antibodies. Arch. Insect Biochem. Physiol. 5:99-118.
Crossref
 
Boman HG, Hultmark D (1987). Cell-free immunity in insects. Ann. Rev. Microbiol. 41:103-114
Crossref
 
Bradford MM (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem. 72:248-254.
Crossref
 
Brehelin M, Drif L, Baud L, Boemare N (1989). Insect haemolymph: co-operation between humoral and cellular factors in Locusta migratoria. Insect Biochem. 19:301-307.
Crossref
 
Carton Y, Nappi AJ (1997). Drosophila cellular immunity against parasitoids. Parasitol. Today 13(6):218-227.
Crossref
 
Cerenius L, Söderhäll K (2004). The prophenoloxidase-activating system in invertebrates. Rev. Immunol. 198:116-126.
Crossref
 
Cerenius L, Babu R, Söderhäll K, Jiravanichpaisal AP (2010). In vitro effects on bacterial growth of phenoloxidase reaction products. Inverteb. Pathol. 103:21-23.
Crossref
 
Chase MR, Raina K, Bruno J, Sugumaran M (2000). Purification, characterization and molecular cloning of prophenoloxidase from Sarcophaga bullata. Insect Biochem. Mol. Biol. 30:953-967.
Crossref
 
Cherqui A, Duvic B, Brehelin M (1996). Purification and Characterization of prpphenoloxidase from the hemolymph of Locusta migratoria. Arch. Insect Biochem. Physiol. 32:225-235.
Crossref
 
Dularay B, Lackie AM (1985). Haemocytic encapsulation and the prophenoloxidase activation pathway in the locust Schistocerca gregaria Forsk. Insect Biochem.15:827-834.
Crossref
 
Dunphy GB (1991). Phenoloxidase activity in the serum of two species of insects, the gypsy moth, Lymantria dispar (Lymantriidae) and the greater wax moth, Galleria mellonella (Pyralidae). Comp. Biochem. Physiol. 98B:535-538.
Crossref
 
Durrant HJ, Ratcliffe NA, Hipkin CR, Aspan A, Söderhäll K (1993). Purification of the prophenoloxidase enzyme from haemocytes of the cockroach Blaberus discoidalis. Biochem. J. 289:87-91.
Crossref
 
Fan T, Mingyu L, Jing W, Lingling Y, Rishan C (2009). Purification and characterization of phenoloxidase from Octopus ocellatus. Acta Biochim. Biophys. Sin. 41(10):865-872
Crossref
 
Feng C, Song Q, Lü W, Lu J (2008). Purification and characterization of hemolymph prophenoloxidase from Ostrinia furnacalis (Lepidoptera: Pyralidae) larvae. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 151(2):139-46.
Crossref
 
Fergusson J (1996). Serum Separation from Whole Blood, protocol on line. Department of Biochemistry, University of Nottingham. The Medical School, Q.M.C., Clifton Boulevard, NG7 2UH, U.K.
 
Fujimoto K, Masuda K, Asada N, Ohnishi E (1993). Purification and characterization of prophenoloxidase from the pupae of Drosophila melanogaster. J. Biochem. 113:285-291.
Pubmed
 
Goudru G, Sathish K, Senigala K, Chandish R, Hari C, Kuruba S (2013). Purification and characterization of prophenoloxidase from cotton bollworm, Helicoverpa armigera. Entomol. Res. 43:55-62.
Crossref
 
Gِtz P, Boman HG (1985). Insect immunity. In: Kertkut GA, Gilbert I (eds.), Comprehensive insect Physiology, Biochem. Pharmacol. Pergmon Press, Oxford. pp. 453-485.
 
Harlow E, David P (1988). Antibodies: A Laboratory Manual. pp. 92 -117
 
Hultmark D (1993). Immune reaction in Drosophila and other insects: a model for innate immunity. Trends Genet. 9:178-183.
Crossref
 
Huxham IM, Lackie AM (1989). Behavior in vitro of separated haemocytes from the locust, Schistocerca gregaria. Cell Tissue Res. 251:677-668.
Crossref
 
Kopácek P, Weise C, Gِtz P (1995). The prophenoloxidase from the wax moth Galleria mellonella: purification and characterization of the proenzyme. Insect Biochem. Mol. Biolol. 25:1081-1091.
Crossref
 
Laemmli UK (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
Crossref
 
Leonard C, Kenneth S, Ratcliffe NA (1985). Studies on prophenoloxidase and protease activity of Blaberus craniifer hemocytes. Insect Biochem. 15:803-810.
Crossref
 
Li X, Suzuki K, Kanaori K, Tajima K, Kashiwada A, Hiroaki H (2000). Soft metal ions, Cd(II) and Hg(II), induce triple-stranded alpha-helical assembly and folding of a de novo designed peptide in their trigonal geometries. Protein Sci. 9:1327-1333.
Crossref
 
Lockey TD, Ourth DD (1992). Isolation and characterization of haemolymph phenoloxidase from Heliothis virescens larvae. Comp. Biochem. Physiol. 102:891-896.
 
Meshrif WS, Barakat ES (2002). Cell mediated immunity in the Locust, Shistocerca gregaria against the bacterium, Bacillus thuringiensis. J. Egypt. Acad. Soc. Environ. Dev. 2(1):117-130.
 
Miranpuri S, Khachatourians GG (1993). Haemocyte surface changes in the migratory grasshopper, Melanoplus sanguinipes in response to wounding and infection with Beuveria bassiana. J. Entomol. Exp. Appl. 68:157-164.
Crossref
 
Mo'men SHA, Salama MS, Barakat EMS, Salem DAM (2010). The Role of Prophenoloxidase Activation System in Cellular Defense Mechanisms in the Haemolymph of the Desert Locust, Schistocerca gregaria (Forskal) M.Sc. Thesis, Science Faculty, Ain Shams University.
 
Mo'men SHA, Salem DAM, Barakat EMS, Salama MS (2012). Activation of Prophenoloxidase during Bacterial Injection into the Desert Locust, Schistocerca gregaria. Int. J. Med. Biol. Sci. 6:291-300.
 
Müller HM, Dimopoulos G, Blass C, Kafatos FC (1999). A hemocyte-like cell line established from the malaria vector Anopheles gambiae expresses six prophenoloxidase genes. J. Biol. Chem. 274:11727-11735.
Crossref
 
Nappi AJ, Christensen BM (2005). Melanogenesis and associated cytotoxic reactions: applications to insect innate immunity. Insect Biochem. Mol. 35:443-459.
Crossref
 
Nellaiappan K, Sugumaran M (1996). On the presence of prophenoloxidase in the hemolymph of the horseshoe crab, Limulus. Comp. Biochem. Physiol. 113B: 163 - 168.
Crossref
 
Pang QX, Zhanga SC, Shi X, Sua F, Wua D (2005). Purification and characterization of phenoloxidase from amphioxus Branchiostoma belcheri tsingtauense. Fish Shellfish Immunol. 19:139-148.
Crossref
 
Perdomo MR, Montero A, Perera VE, Pardo RZ, Alonso JE (2007). Phenoloxidase o activity in the hemolymph of the spiny lobster Panulirus argus. Fish Shellfish Immunol. 23:1187-1195.
Crossref
 
Rowley AF, Brookman JL, Ratcliffe NA (2011). Possible involvement of the prophenol-oxidase system of the locust, Locusta migratoria, in antimicrobial activity. Inverteb. Pathol. 60(2):31-38.
 
Ryazanova AD, Alekseev AA, Slephneva IA (2012). The phenylthiourea is a competitive inhibitor of the enzymatic oxidation of DOPA by phenoloxidase. J. Enzyme Inhib. Med. Chem. 27(1):78-83.
Crossref
 
Towbin H, Staehelint T, Gordon J (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. 76 (9):4350-4354.
Crossref
 
Vavricka CJ, Qian H, Prajwalini M, Haizhen D, Bruce MC, Li J (2014). Tyrosine metabolic enzymes from insects and mammals: A comparative perspective. Insect Sci. 21:13-19.
Crossref
 
Yasuhara Y, Koizumi Y, Katagiri C, Ashida M (1995). Reexamination of properties of prophenoloxidase isolated from larval haemolymph of the silk worm, Bombyx mori. Arch. Biochem. Biophys. 320:14-23.
Crossref
 
Zhao P, Li J, Wang Y, Jiang H (2007). Broad-spectrum antimicrobial activity of the reactive compounds generated in vitro by Manduca sexta phenoloxidase. Insect Biochem. 37(9):952-959.
Crossref
 
Zibaee A, Bandani A, Malagoli D (2011). Purification and characterization of phenoloxidase from the hemocytes of Eurygaster integriceps (Hemiptera: Scutelleridae) Compar. Biochem. Physiol. 158B:117-123.
Crossref