October 2011
Temporal expression of genes involved in the biosynthesis of gibberellins in birch (Betula platyphylla)
Gibberellins (GAs) are a large family of endogenous plant growth regulators. Bioactive GAs influence nearly all processes during plant growth and development. In the present study, we cloned and identified 10 unique genes that are potentially involved in the biosynthesis of GAs, including one BpGGDP gene, two BpCPSgenes, one BpKS gene, two BpKO genes,...
October 2011
Morphological characterization and phylogenetic distance among several genotypes of Rebutia, Aylostera, Mediolobivia and Sulcorebutia (Cactaceae)
Four genera and 75 species belonging to Cactaceae family were investigated regarding their morphology and their molecular polymorphism. The botanical classification that described the phenotypic aspects of different characters, such as number of spines/areoles, length of spines and flower diameter, was used to describe the main peculiarities (morphological method). In Rebutia genus, the floral...
October 2011
Correlation and path-cofficient analysis of seed yield and yield related trait in Iranian confectionery sunflower populations
This study was undertaken in order to determine the association among yield components and their direct and indirect effects on the seed yield of confectionery sunflower. 36 confectionery sunflower populations originated from different regions of Northwest Iran were characterized using 11 agro-morphological traits including: Days to 50% flowering, plant height, stem diameter, head diameter, number of leaves, leaf...
October 2011
Application of randomly amplified polymorphic DNA (RAPD) markers and polyphenol oxidases (PPO) genes for distinguishing between the diploid (glaucum) and the tetraploid (leporinum) accessions in Hordeum murinum complex
The genome of 12 wild barley accessions belonging to Hordeum murinum complex (eight out of them belong to H. m. glaucum and four belong to H. m. leporinum) was characterized using RAPD-PCR markers and polyphenol oxidases genes (PPO). A total of 123 RAPD markers with 22 arbitrary decamer primers were used. 56 out of the RAPD markers were common to all accessions, and the other 67...
October 2011
Genetic analysis of field and physiological indicators of drought tolerance in bread wheat (Triticum aestivum L.) using diallel mating design
In order to study the inheritance of field, physiological and metabolite indicators of drought tolerance in wheat, an eight-parental diallel cross, excluding reciprocals, was grown in a randomized complete block design (RCBD) with three replications under two different water regimes (irrigated and rainfed). Significant differences were found for yield potential (Yp), stress yield (Ys), stress tolerance index (STI), leaf...
October 2011
New allelic variant of the ovine calpastatin gene
Calpastatin, a specific inhibitor of the calpains, participates in muscle growth and tenderization. Amplified fragment of 12 intron of CAST was used; the gene encoding calpastatin, from 164 lambs from synthetic lines for restriction fragment length polymorphism analysis. Although, CAST has previously been investigated in sheep, the observed polymorphism incidence in this...
October 2011
Evaluation of sorghum genotypes under drought stress conditions using some stress tolerance indices
Seven genotypes of sorghum (Sorghum bicolour (L.) Moench) were studied in both drought and normal conditions. In each condition, the genotypes were evaluated using a split plot based randomized complete block design with three replications. Drought tolerance indices including stability tolerance index (STI), mean productivity (MP), geometric mean productivity (GMP), stress susceptibility index (SSI), tolerance...
October 2011
Effect of zinc fertilizer applications on yield and element contents of some registered chickpeas varieties
In this study, the effect of zinc fertilizer applications on different chickpea varietiesand the most suitable zinc application dose were investigated under the field conditions in May to September of 2003 and 2004. Three types of chickpeas (Cicer arietinum cv.Canitez-87, cv. ILC-482 and cv. Gokce) were studied under irrigated conditions. Zinc was applied to the soil at four doses 0, 0.5, 1.0 and 1.5 kg...
October 2011
Influence of prohexadione-calcium, trinexapac-ethyl and hexaconazole on lodging characteristic and gibberellin biosynthesis of rice (Oryza sativa L.)
We investigated the influence of prohexadione-calcium (Pro-Ca), trinexapac-ethyl (TNE) and hexaconazole (HX) on lodging and gibberellin (GA) biosynthesis pathway of rice cultivar, Hwayeongbyeo. It was observed that these novel synthetic growth retardants suppressed lodging of rice under field conditions through blocking GA biosynthesis pathway. These growth retarding chemicals were applied at basic (20 uM) and...
October 2011
Effect of land use patterns on stability and distributions of organic carbon in the hilly region of Western Sichuan, China
Soil aggregation is important for the resistance of land surfaces to erosion, and it influences the ability of soils to remain productive. At the same time, it is also an important process of carbon sequestration. The objectives of this study were to elucidate the effects of different land use patterns on soil aggregate stability and the distribution of organic carbon in different aggregate fractions in order to prove...
October 2011
Cultivation of the microalga, Chlorella pyrenoidosa, in biogas wastewater
Biogas wastewater is always a problem as a result of its extremely high concentrations of nitrogen and phosphorus, which is the main reason for the eutrophication of the surrounding water. The microalga, Chlorella pyrenoidosa, can utilize the nitrogen and phosphorus in wastewater for its growth. Therefore, the microalga was introduced to be cultivated in the biogas wastewater, which could not only...
October 2011
The effects of gold nanoparticles size and concentration on viscosity, flow activation energy, dielectric and optical properties
This study was carried out to investigate viscosity in relation with the temperature, flow activation energy and dielectric properties for 10, 20 and 50 nm gold nanoparticles size (GNPs) in addition to absorption and fluorescence spectra at different concentrations (0.2 × 10-3 to 1 × 10-2%) in an attempt to cover and understand the toxicity and potential role of their...
October 2011
Studies on the growth behavior of Chlorella, Haematococcus and Scenedesmus sp. in culture media with different concentrations of sodium bicarbonate and carbon dioxide gas
Growth studies were conducted on green algae Chlorella, Scenedesmus andHaematococcus strains in batch mode cultures. In this study, the effect of sodium bicarbonate salt (NaHCO3) and carbon dioxide (CO2) gas as carbon source on microalgal cultures were investigated. For this purpose, growth response of theaforementioned three strains under varying concentrations of NaHCO3 (15, 30, 45, 60...
October 2011
Production and characterization of lipase from Bacillus stearothermophilus
The production of lipase by Bacillus stearothermophilus was investigated and its properties were evaluated. Different factors such as temperature, pH, carbon source, incubation period and culture volume were studied for improving lipase production. B. stearothermophilus was able to produce lipase at a wide range of temperature and pH values. The maximum enzyme activity of...
October 2011
Process parameters influencing tannase production by Aspergillus niger using mangrove (Rhizophora apiculata) bark in solid substrate fermentation
Rhizophora apiculata bark is a tannin-rich waste material obtained from charcoal industry. This industrial waste was used as solid substrate in the study for the production of tannase and at the same time, help in minimizing the country’s industrial wastes. This study was carried out to optimize the physical parameters for the maximal tannase production by Aspergillus niger via solid substrate...
October 2011
Studying arsenic trioxide-induced apoptosis of Colo-16 cells with two-photon and confocal laser scanning microscopy
With two-photon and confocal laser scanning microscopy in combination with fluorescent probes Hoechst 33342, 2¢,7¢-dichlorofluorescin diacetate (DCFH-DA) and Fluo 3-AM, we simultaneously observed arsenic trioxide (As2O3)-induced changes in nuclear morphology, reactive oxygen species (ROS) and intracellular calcium concentration (Ca2+)i within human skin squamous carcinoma cells (Colo-16 cells). Our...
October 2011
Growth effects on mixed culture of Dunaliella salina and Phaeodactylum tricornutum under different inoculation densities and nitrogen concentrations
Dunaliella salina and Phaeodactylum tricornutum are two important marine microalgae rich in bioactive substances and other high-value constituents. In thisstudy, growth effects on mixed culture of these two microalgae were studied under different inoculation proportions (10:0, 7:3, 5:5, 3:7, 0:10) and low, medium and high nitrogen concentrations of 1.4, 14 and 140 mg/l, respectively....
October 2011
Biochemical properties and potential applications of an organic solvent-tolerant lipase isolated from Bacillus cereus BF-3
An organic solvent-tolerant strain producing lipase was isolated. The optimal pH and temperature were 8.0 and 34°C, respectively. The lipase was stable at 50°C after 1 h incubation at pH 6 to 8. BF-3 lipase was very stable when incubated with methanol, ethanol and toluene below 30% (v/v) but was stability reduced drastically above 50%. While K+, Na+, Mg2+ and Ba2+ (1 mMol/L; 3 mMol/L) stimulated lipase...
October 2011
Heterologous expression and partial purification of calcium and calmodulin dependant protein kinase (CCaMK) of Medicago truncatula
In legumes, the establishment of symbioses with arbuscular mycorrhizal fungi and nitrogen-fixing bacteria share a common signalling pathway. One of the shared components is a calcium and calmodulin dependant protein kinase predicted to perceive and transduce the calcium signals generated upon perception of the symbiotic signals. The removal of the autoinhibitory domain that negatively...
October 2011
Decreasing erucic acid level by RNAi-mediated silencing of fatty acid elongase 1 (BnFAE1.1) in rapeseeds (Brassica napus L.)
The β-ketoacyl CoA synthase encoded by fatty acid elongase 1 gene (BnFAE1.1) is a rate-limiting enzyme regulating biosynthesis of erucic acid in rapeseeds (Brassica napus). To develop low level of erucic acid in rapeseeds by intron-spliced hairpin RNA, an inverted repeat unit of a partial BnFAE1.1 gene interrupted by a spliceable intron was cloned into pCAMBIA3301, and a seed-specific...
October 2011
Antibacterial activity of crude extracts from Mexican plants against methicillin-resistant Staphylococcus
The aim of this study was to evaluate the antimicrobial activity of 36 extracts from 18 vegetal species used as soap, insecticides, insect repellent and for the treatment of several diseases likely associated to microorganisms. The vegetal species were collected in Oaxaca, Puebla and Veracruz States, México. The extracts were evaluated against isolates of nosocomial infections...
October 2011
Casein kinase 1-Like 3 is required for abscisic acid regulation of seed germination, root growth, and gene expression in Arabidopsis
The homozygous T-DNA mutant of a casein kinase 1-Like 3 (ckl3) was identified. The quantitative real-time polymerase chain reaction (QRT-PCR) results showed that CKL3 was highly expressed in flowers and roots, but less in stems, leaves and leafstalks. It was found that CKL3 gene was induced by abscisic (ABA). When grown in the presence of increasing concentration of...
October 2011
Adipose tissue-derived stem cells in oral mucosa tissue engineering: Enhanced migration and proliferation in co-culture with oral keratinocytes in vitro
Tissue-engineered oral mucosa holds a great prospect in urethroplasty and adipose tissue-derived stem cells (ADSCs) may play an important role in this field. In this research, canine oral keratinocytes (OKs) and ADSCs were harvested and culturedin vitro. The affinity between the two cell lines was evaluated by analyzing their migration and proliferation patterns in a co-culture environment. The results demonstrate that...
October 2011
Effect of artemether on rat hepatocytes during acute damage
The aim of this study was to investigate the hepatocellular regeneration ability of artemether in experimental CCl4-induced acute damage in rat hepatocytes. 20Wistar rats were equally allocated to 4 groups. The first group was designated as the distilled water control group (group 1); the second group was CCl4 toxic control (group 2) and received oral administration of CCl4 (50 mg/kg body weight diluted...
October 2011
Rhein induces apoptosis of HCT-116 human colon cancer cells via activation of the intrinsic apoptotic pathway
Rhein, a major compound in rhubarb, has been found to have anti-tumor properties in many human cancer cells. However, the details about rhein suppressing the growth of human colon cancer cells remained elusive. In this paper, we explored the potential of rhein as a chemotherapeutic agent on HCT-116 cells and demonstrated significant inhibition of HCT-116 cells proliferation in both concentration (0, 10, 30, and 100...
October 2011
Rotational and nematicidal effect of lupine (Lupinus albus L.: Leguminosae)
The study was conducted to evaluate the nematicidial and rotational activity of lupine (Lupinus albus L.), in completely randomized design in a growth room in pots, in 2011. Naturally infested soil, from an arable land containing root-lesion nematode Pratylenchus thornei Sher and Allen including several other genera of free-living was used. Firstly, lupine (L. albus) and wheat (Tricum durum- the variety...
October 2011
The impact of microwaves irradiation and temperature manipulation for control of stored-products insects
The impact of microwaves irradiation and temperature manipulation against adults of saw-toothed grain beetle, Oryzaephlius surinamensis (L.) and cigarette beetle,Lasioderma serricorne (F.), over various exposure times and cold storage period was evaluated. The insects were exposed to 2450 MHZ at five different power levels of 0, 100, 200, 300 and 400 W for five exposure times of 0, 3, 6, 9 and 12 min. A...
October 2011
The implement of plastic oval tags for mark-recapture in juvenile Japanese flounder (Paralichthys olivaceus) on the northeast coast of Shandong Province, China
As part of the stock enhancement research project of Shandong Province, China, plastic oval tags (POTs) were used to mark juvenile Japanese flounder for release,Paralichthys olivaceus (70 to 133 mm total length, TL), in 2009 and 2010. Optimal tag placement locations, retention, tagging rates, and mortality were initially evaluated. Mark–recapture experiments were carried out in the coastal waters of Weihai...
October 2011
Development of loop-mediated isothermal amplification method for visualization detection of the highly virulent strains of porcine reproductive and respiratory syndrome virus (PRRSV) in China
A novel assay method to detect the highly virulent Porcine reproductive and respiratory syndrome virus (PRRSV) termed reverse transcriptase loop-mediated isothermal amplification (RT-LAMP), was reported by using hydroxynaphthol blue (HNB) as the LAMP product colorimetric judgment. By the set of special primers, targeting the sequence that belongs to Nsp2 gene containing an 87 bp deletion mutation,...
October 2011
Optimized conditions for high-level solubilization and purification of recombinant camel growth hormone in Escherichia coli
In this report, we describe the cloning, over-expression, efficient solubilization, purification and evaluation of bioactivity of camel growth hormone (cGH). The total cellular RNA was extracted from pituitary glands of freshly slaughtered animals and cDNA of cGH was synthesized by a pair of sequence specific primers with a product of 576 base pairs (bps). Amplicons was cloned into T/A cloning...
October 2011
Effect of Agri-SC as a soil conditioner on runoff, soil loss and crust strengths
This study was carried out to determine the effect of Agri-SC as a soil conditioner at different doses (0, 18.50, 37.00, 55.50 and 74.00 l ha-1) on water erosion and crust strengths under laboratory conditions with three replicates. The Agri-SC solutions were sprayed and two consecutive simulated rainfalls (60 mm h-1) were applied on a loamy soil sample into the erosion plots. Erosion plots were waited under a platform...
October 2011
Biodegradation and growth characteristics of a toluene-degrading strain
A toluene-degrading strain was isolated from active sludge in this study. Both growth characteristic and the performance to degrade toluene by the strain in batch culture mode were evaluated. Results showed that the isolated strain presented a good ability to remove toluene with the maximum removal efficiency of 93.8%. Growth and toluene degradation occurred at 20 to 50°C but the optimum was found to be...
October 2011
Analysis of chemical constituents in medicinal plants of selected districts of Pakhtoonkhwa, Pakistan
Alkaloids, flavonoids and saponins were determined in the Teraxacam officinale,Cichorium intybus and Figonia tritica. Quantative determinations of crude alkaloids, flavonoids and saponins (g kg-1) and their percentages (%) were determined in C. intybus, T. officinale and F. critica collected from Kohat, Mardan, Nowshera and Peshawar regions. Thin layer chromatography (TLC) study...
October 2011
Some biological studies on Hypnea pannosa J. Ag.
The present investigation focused on studying the toxicity, analgesic, behavioral and anti-emetic activities of the ethanol extract of Hypnea pannosa. The ethanol extract caused 100% lethality at the doses of 250 and 500 mg/kg. Significant analgesic and central depressant effects were observed from 150 mg/ kg dose. The extract at 200 mg/kg dose exhibited significant anti-emetic effect, when compared to...
October 2011
Efficiency of different levels of Satureja hortensis L. (Savory) in comparison with an antibiotic growth promoter on performance, carcass traits, immune responses and serum biochemical parameters in broiler chickens
This study was conducted to examine the effects of different levels of Satureja hortensis L. (Savory) in comparison with an antibiotic growth promoter (flavophospholipol) on performance, carcass characteristics, immune responses and serum biochemical parameters of broiler chicks. In this study, 240 one-day-old mixed sex broiler chicks (Ross 308) were weighed and randomly allocated to the 4...
October 2011
Estimation of genetic parameters in three commercial silk-worm lines of Iran
In order to estimate genetic parameters of the cocoon weight (CW), cocoon shell weight (CSW) and cocoon shell percentage (CSP) of three industrial lines of silk-worm (number 31, 103 and 107), data were collected from three successive generations. (Co)variance components were as follows: additive genetic variation (δ2g) and environmental variation (δ2e) of traits and additive genetic covariance (covg1, 2) and...
October 2011
Genetic polymorphism of exon 9-11 of the leptin gene receptor in breeder hens of Mazandaran native fowls
Leptin is a 16 kDa protein synthesized by white adipose tissue and involved in regulation of feed intake, energy balance, fertility and immune function. In order to evaluate the leptin gene receptor polymorphism, we used a restriction fragment length polymorphism (RFLP) method. Blood samples were collected from 100randomly chosen Mazandaran native fowls. Genomic DNA was extracted using modified salting-out method and...
October 2011
Determination of taste receptor type 1 member 1 (TAS1R1) gene polymorphism and association with some body measurements in goats
Umami taste plays a key role in intake of amino acids. It is believed that the taste receptor type 1 member 1 (TAS1R1) and taste receptor type 1 member 3 (TAS1R3) receptors are to function in combination as a heteromeric umami taste receptor in humans and other mammals. Most of the previous studies laid particular emphasis on the taste sensitivity to umami substances or the relation of umami taste sensitivity to...
October 2011
Effects of dietary crude protein and calcium/phosphorus content on growth, nitrogen and mineral retention in broiler chickens
This experiment was conducted to examine the effect of three levels of crude protein (CP) (NRC, 15% more than NRC and 15% less than NRC) and three levels of Ca and available P (Av. P) (NRC, 15% more than NRC and 15% less than NRC) on performance of broilers from hatching until 21 days of age. The experimental design was a completely randomized design (CRD), with a 3 × 3 factorial arrangement of nine...
October 2011
Detection of polymorphism of the insulin-like growth factor-I (IGF-I) gene in Mazandaran native chicken using PCR-RFLP method
Molecular genetic selection on individual genes is a promising method to genetically improve economically important traits in chickens. The insulin-like growth factor-I (IGF-I) gene may play important roles in growth of multiple tissues, including muscle cells, cartilage and bone. In the present study, polymorphism of the promoter and 5' untranslated region of IGF-I gene of Mazandaran native fowls was...
October 2011
Developing knowledge level scale of functional foods: Validity and reliability study
The aim of the study was to develop a scale to determine the knowledge levels of University students on functional foods and to investigate the validity and reliability of the scale. The research was conducted on 417 (209 girls and 208 boys) undergraduate students in Selcuk University regarding functional foods. The participants were selected from different departments of Selcuk University using proportional cluster...
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