July 2013
Bioinformatics tools for development of fast and cost effective simple sequence repeat (SSR), and single nucleotide polymorphisms (SNP) markers from expressed sequence tags (ESTs)
The development of current molecular biology techniques has led to the generation of huge amount of gene sequence information under the expressed sequence tag (EST) sequencing projects on a large number of plant species. This has opened a new era in crop molecular breeding with identification and/or development of a new class of useful DNA markers called genic molecular markers (GMMs). These markers represent...
July 2013
Genome-wide identification, functional analysis and expression profiling of pleiotropic drug resistance (PDR) sub-family in potato
The plant pleiotropic drug resistance (PDR) family of ATP-binding cassette (ABC) transporters has comprehensively been researched in relation to transport of antifungal agents and resistant pathogens. In our study, analyses of the whole family of PDR genes present in the potato genome were provided. This analysis resolves discrepancies of potato PDR proteins and provides an expression analysis of all annotated potato...
July 2013
DNA profiling of commercial chilli pepper (Capsicum annuum L.) varieties using random amplified polymorphic DNA (RAPD) markers
In the present study, genetic variability in 10 commercial chilli pepper varieties viz. Gemini, G-334, Agnirekha, Pusa jwala, Mangala, Black diamond, Sindura, Pusa jyothi, Badiga-2 and Teja (branch), cultivated in the local area of Andhra Pradesh State, India, using random amplified polymorphic DNA (RAPD) markers was examined. Out of thee RAPD markers used for screening 10 chilli pepper genotypes,...
July 2013
Genetic relationships of cotton (Gossypium barbadense L.) genotypes as studied by morphological and molecular markers
Fifteen (15) morphological traits and three different types of molecular markers [inter simple sequence repeats (ISSR), simple sequence repeat (SSR) and expressed sequence tag (EST) markers] were used to study the genetic relationships among 24 cotton (Gossypium barbadense L.) genotypes (commercial varieties and new germplasm). High significant differences were observed among the genotypes for all the studied...
July 2013
Genetic diversity in cocoa (Theobroma cacao L.) plus trees in Tamil Nadu by simple sequence repeat (SSR) markers
The range of polymorphism of about 27 cocoa plus trees screened in the major cocoa growing regions of Tamil Nadu were assessed using 10 simple sequence repeat (SSR) markers. The gene diversity, genetic differentiation and genetic similarities were analyzed for the cocoa trees. The number of alleles detected by different primers ranged from 0 to 3 and the level of polymorphism was 0 to 100%. The polymorphism information...
July 2013
Construction and prokaryotic expression of the fusion gene PRRSV GP5 and Mycobacterium bovis Hsp70
Porcine reproductive and respiratory syndrome (PRRS) is an economically important swine disease that has devastated the swine industry worldwide. Vaccination with live attenuated vaccine or inactivated vaccine is the main treatment to control PRRS. However, the disadvantages such as virulence resumption of the attenuated vaccine and low immunogenicity of the inactivated vaccine call for a more efficient and safer...
July 2013
Comparison of protocols for genomic DNA extraction from ‘velame pimenta’ (Croton linearifolius), a native species to the Caatinga, Brazil
The Caatinga biome occupies some 12% of the Brazilian territory, which is present in at least nine states. The species that constitute its biodiversity have the potential to be used as natural resources, among them are approximately 700 species of the genusCroton. As an example of this potential, the Croton linearifolius specie is used by local communities as a natural insecticide. Associated with the economic...
July 2013
Manganese analysis of sorghum hybrid (Sorghum bicolor (L) Moench) on different lamp current using an atomic absorption spectrophotometer
Several studies on the effect of water treatment sludge (WTS) have been conducted on trace metal contents of sorghum hybrid (Sorghum bicolor (L.) Moench) grown on a mountainous Kumsan district in the Republic of Korea. The objective of the present study was for measuring manganese (Mn) content in sorghum hybrid on an appropriate lamp current with an atomic absorption spectrophotometer. Four treatments...
July 2013
Effect of seasonal drawdown variations on groundwater quality in Nigeria
Water samples from twenty (20) shallow wells in Akure were analyzed during the wet and dry seasons in 2009 to ascertain the effect of drawdown on their qualities. Twenty (20) parameters consisting of five physical, twelve chemical and three heavy metals were tested for in the samples. The parameters included dissolved and suspended solids, turbidity, the pH, alkalinity, calcium, sulphate, nitrate, magnesium, electrical...
July 2013
Morphological and anatomical responses of two Palestinian tomato (Solanum lycopersicon L.) cultivars to salinity during seed germination and early growth stages
The effect of salinity (50, 100 and 150 mM NaCl) on seed germination and early growth of two Palestinian tomato (Solanum lycopersicon) cultivars (J1 and Ram) was investigated. Salinity delayed seed germination of both cultivars, and none of them reached 50% germination at 150 mM NaCl even after 21 days of incubation. The seedling height increased with time but decreased with increasing salinity level in both...
July 2013
Screening of in vitro antioxidant activity of bark extracts of Polyalthia longifolia
The purpose of this study was to evaluate the antioxidant activity of the bark extracts of Polyalthia longifolia. Methanol, ethanol and aqueous extracts of bark of P. longifoliawere evaluated 1,1-diphenyl-1,2-picryl hydroxyl (DPPH) radical scavenging, hydrogen peroxide scavenging, hydroxyl radical scavenging, superoxide radical scavenging, total phenolic content, total antioxidant capacity, and...
July 2013
Effectiveness of gamma ray irradiation and ethyl methane sulphonate on in vitro mutagenesis of strawberry
The experiment was conducted to study the effect of gamma-ray irradiation on the high concentration thidiazuron (TDZ) produced buds. In vitro buds were irradiated with different gamma-ray doses. Akihime cultivar (‘Akihime’) was irradiated with the doses of 0, 30, 80, 130, 180, and 230 Gy while ‘DNKW001 accession’ (‘DNKW001’) was exposed to the doses of 0, 30, 80, 130, 180,...
July 2013
In vitro callus formation in cultivated and wild species of Cyamopsis
2,4-Dichlorophenoxy acetic acid (2, 4-D) and benzylaminopurine (BAP) induced callusing from cotyledons in all three species of Cyamopsis. The maximum callus induction from cotyledon explant was evident in Cyamopsis serrata and Cyamopsis senegalensis on a medium supplemented with 2,4-D (2 mg/l). On the other hand,Cyamopsis tetragonoloba showed poor callus formation on the same...
July 2013
Optimization of coagulation-flocculation process for printing ink industrial wastewater treatment using response surface methodology
A coagulation–flocculation process was used to treat water-based printing ink wastewater with aluminum sulphate {Al2(SO4)3} as coagulant and with Praestol as flocculant. To minimize turbidity and sludge volume index (SVI), the experiments were carried out using jar tests and response surface methodology (RSM) was applied to optimize this process. A central composite design, which is the standard design of RSM, was...
July 2013
An extensive characterization study of different Bacillus thuringiensis strains collected from the Nashville Tennessee area
Bacillus thuringiensis (Bt) is a delta-endotoxins producing bacterium that inhibits the digestive process of many insect orders such as; lepidopteran, dipteran and coleopteran. A comparative study between sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis and DNA hybridization was performed on 25 chosen different strains of B. thuringiensis. Based on patterns from these molecular tools, the...
July 2013
Antibacterial evaluation of Anacardium occidentale (Linn) (Anacardiaceae) in semiarid Brazil
Microorganisms that cause losses are proving to be resistant to most known antibiotics, thereby encouraging the search for naturally occurring antibiotics. This study aimed to perform a phytochemical and bacterial study of ethanolic extracts of leaves and barks of Anacardium occidentale L. The samples were submitted for extraction using ethyl alcohol; the crude extract was used to perform phytochemical...
July 2013
Quantification of microcystin-producing microcystis in freshwater bodies in the Southern Mozambique using quantitative real time polymerase chain reaction
In the last decades, large number of reported cases of illnesses in Mozambique is related to drinking water. However, only a limited number of studies have focused on aquatic pollution in this country. Cyanobacterial blooms dominated by Microcystis sp are regularly identified in freshwater bodies in Mozambique. Microcystis is known to proliferate in freshwater bodies and produce microcystins which...
July 2013
Physico-chemical assessment of borehole water used by schools in Greater Giyani Municipality, Mopani district, South Africa
In the present study, the physical and chemical quality of several borehole water sources, used by rural schools in Greater Giyani Municipality, were assessed to determine their safety for human consumption. Atomic absorption spectroscopy and ion chromatography were used to determine the chemical quality of water sources. The pH of the water samples varied between 5.29 and 8.3 and tended to be lower in summer and higher...
July 2013
Extracts of Moringa oleifera Lam. showing inhibitory activity against early steps in the infectivity of HIV-1 lentiviral particles in a viral vector-based screening
Moringa oleifera Lam. (Moringaceae) is one of the many medicinal plants employed by herbalist to treat or manage people living with HIV/AIDS (PLWHA) in African Traditional Medicine (ATM) and there are many claims to the fact that it improves quality of life and reverses the course of the HIV/AIDS disease progression. This practice and the claims of efficacy spurred the present study in which the...
Advertisement
Advertisement
