African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12267

Table of Content: 28 September, 2016; 15(39)

September 2016

Molecular identification of Anopheles gambiae sensu stricto Giles (formerly Anopheles gambiae Savannah Form) in Kamuli District, Uganda

Anopheles gambiae sensu stricto Giles (formerly A. gambiae S molecular form), the largely anthropophilic species, is reportedly the most important malaria vector in Uganda among the A. gambiae complex species. Indoor and outdoor human-biting mosquitoes were caught for four consecutive nights in each of 48 households in Kamuli district using human-baited bed net traps for subsequent identification of the principal...

Author(s): Fredrick George Kabbale, Anne Margaret Akol, John Baptist Kaddu, Enock Matovu, Anne Kazibwe, Anges Yadouleton and Ambrose Winston Onapa

September 2016

In vitro methods for mutation induction in potato (Solanum tuberosum L.)

Potato (Solanum tuberosum L.) is an important vegetable and staple crop worldwide and mainly propagated vegetatively. Breeding of potato is problematic and therefore induced mutation is an attractive means of improving the crop. In vitro culture systems, and especially the production of micro-tubers, are ideal for such purposes in potato improvement. Radio-sensitivity testing (growth reduction, GR and lethal dose, LD)...

Author(s): Souleymane Bado, Matumelo Alice Rafiri, Kaoutar El-Achouri, Enoch Sapey, Stephan Nielen, Abdelbagi Mukhtar Ali Ghanim, Brian Peter Forster, and Margit Laimer

September 2016

Natural occurrence and pathogenicity of Xanthomonas bacteria on selected plants

The bacterial genus Xanthomonas consists of several species of economic importance, among which Xanthomonas campestris pv.musacearum (Xcm), the cause of enset and banana wilt is the most important in tropical Africa. However, the natural occurrence and host range of this species is yet to be clarified. The objectives of this study were to verify the presence of Xanthomonas bacteria on plants growing in and around...

Author(s): Alemayehu Chala, Tadesse Kebede and Guy Blomme

September 2016

Development of simple kinetic models and parameter estimation for simulation of recombinant human serum albumin production by Pichia pastoris

In order to describe and predict the growth and expression of recombinant proteins by using a genetically modified Pichia pastoris, we developed a number of unstructured models based on growth kinetic equation, fed-batch mass balance and the assumptions of constant cell and protein yields. The growth of P. pastoris on both glycerol and methanol could be represented by Monod kinetic equation. A simple simulation...

Author(s): Panchiga Chongchittapiban, Jӧrgen Borg, Yaowapha Waiprib, Jindarat Pimsamarn and Anan Tongta

September 2016

Improved embryogenic callus induction and plant regeneration in big bluestem (Andropogon gerardii Vitman), a potential bioenergy feedstock

The objective of this study was to develop an efficient regeneration protocol in big bluestem, a potential feedstock that produces huge biomass. Embryogenic calli were induced from the seeds of cultivars, Kaw and Earl, on Murashige and Skoog (MS) medium with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) (0.2, 0.5, 1.0, 2.0, 3.0 and 5.0 mg l-1) alone or in combination with 6-benzylaminopurine (BA)...

Author(s): Pramod Pantha, Sathish Kumar Ponniah, Sixte Ntamatungiro and Muthusamy Manoharan

September 2016

A novel sampler for limnological investigation in developing world

A simple pump sampler for shallow water bodies, constructed with materials that are affordable and readily available in the local market is presented. It is simple to operate and no electric motor is required. The challenge of cost of acquisition of conventional samplers for sampling the lentic habitat, especially small water bodies such as small natural lakes, rivers, streams and reservoirs for fisheries and water...

Author(s): Nkechinyere O. Nweze

September 2016

Evaluation of the simultaneous effects of a heat stabilized starter concentration and the duration of fermentation on the quality of the opaque sorghum beer

This study evaluates the simultaneous effect of dried starter concentration and fermentation duration on the quality characteristics of the African opaque sorghum beer using response surface methodology. The aim was to improve the beer quality and to optimize its fermentation process. Results show that the granule starter concentration and the fermentation duration have significant effects on the dry matter, refractive...

Author(s): Yves Kadjogbé Djegui, Adéchola Pierre Polycarpe Kayodé, Joseph Dossou and Joseph Djidjoho Hounhouigan

September 2016

Preliminary investigation into the chemical composition of the invasive brown seaweed Sargassum along the West Coast of Ghana

The arrival of the invasive brown seaweed Sargassum in the Western Region of Ghana was first reported in 2009. This impacted negatively on biodiversity, tourism and the livelihoods of the coastal communities. The objectives of the study among others were to identify and determine the nutritional and toxicological contents of the seaweed. Twenty four samples collected from 6 zones along the Western Region were analysed....

Author(s): Gloria Naa Dzama Addico and Kweku Amoako Atta deGraft-Johnson