July 2014
Genetic variation and DNA markers in forensic analysis
In this review, we highlighted the DNA markers and their applications in forensic medicine which included the study of genetic variation or polymorphism, markers for genetic variation, types of variation, diallelic polymorphisms (SNPs and Indels), haplotypes, autosomal short tandem repeat (STRs), Y-chromosome short tandem repeat (Y-STRs) and Y chromosome. This is a useful tool for tracing human evolution,...
July 2014
Molecular characterization and diversity analysis in chilli pepper using simple sequence repeats (SSR) markers
India is considered to be the secondary center of diversity of chilli pepper, especially of Capsicum annuum. Simple sequence repeats (SSRs) are the most widely used marker system for plant variety characterization and diversity analysis especially in cultivated species which have low levels of polymorphism. The diversity analysis of 64 chilli pepper accessions, mostly of Indian origin, was performed using 50 SSR...
July 2014
Distribution and molecular detection of apple mosaic virus in apple and hazelnut in Turkey
Apple mosaic virus (ApMV) is one of the most important diseases limiting the production of hazelnut and apple in Turkey and the objectives of this research were to determine the convenient and reliable method for RNA isolation and also to determine primer pair for real time polymerase chain reaction (RT-PCR) detection of coat protein gene for Turkish ApMV isolates. Apple mosaic virus isolates were collected in 2007 to...
July 2014
Cloning and characterization of pre-miR159a and pre-miR1123 from Indian hexaploid wheat and their evolutionary linkage analysis
MicroRNAs (miRNAs) are important post-transcriptional regulator of genes in plants. In spite of their unusual small size (-21-24 nt), the evolutionary history of miRNA gene families appears to be similar to their protein-coding counterparts. Although many miRNA genes are conserved across the plant species, the same gene family varies significantly in size and genomic organization in different species. In the present...
July 2014
Preparation of chitosan nanoparticles for loading with NPK fertilizer
In this paper the preparation of chitosan nanoparticles was carried out using methacrylic acid (MAA) and studied by both ultraviolet (UV)- visible transmission spectrophotometry and transmission electron microscopy (TEM). Nanoparticles with sizes as small as 17 to 25 nm were achieved. The obtained nanoparticles had a homogeneous morphology showing a quite uniform particles size distribution with a spherical shape. The...
July 2014
Association mapping of resistance to Verticillium wilt in Gossypium hirsutum L. germplasm
Verticillium wilt is a major disease affecting the growth of cotton. For screening the resistant genes, 320 Gossypium hirsutum germplasms were evaluated in Verticillium nursery, and association mapping was used to detect the markers associated with the Verticillium wilt resistance. 106 microsatellite marker primer pairs were used to estimate the genetic diversity, population structure and linkage disequilibrium (LD) of...
July 2014
Biochemical and secondary metabolites changes under moisture and temperature stress in cassava (Manihot esculenta Crantz)
Cassava’s (Manihot esculenta Crantz) importance as a food security crop in Sub Saharan Africa is enhanced by its special traits such as tolerance to drought and high yields under drought stress. However, full understanding of tolerance mechanisms under hydrothermal stress in cassava is a key in developing highly tolerant varieties with increased yield. In our study, the effects of low soil moisture and increased...
July 2014
Agrobacterium mediated genetic transformation of popular Indica rice Ratna (IET 1411)
This is the first report of genetic transformation of indica rice cultivar Ratna (IET 1411). Agrobacterium tumefaciens strain LBA 4404 carrying a binary vector pCAMBIA1301 harboring the CaMV35S promoter driven modified GUS gene was used. Various parameters critical to rice transformation were optimized including callus induction medium, bacterial concentration, co-cultivation conditions, concentration of the plant...
July 2014
Antibacterial activity of Waltheria indica Linn (Sterculiaceae), collected from Blouberg area, Limpopo Province, South Africa
Mature roots of Waltheria indica were collected from Blouberg area, Limpopo Province, republic of South Africa. Water, ethanol and methanol extracts were investigated for antibacterial activity at 5 mg/ml against 10 human pathogenic strains using disc diffusion method. Ethanol extract exhibited potent antibacterial activity against selected strains while methanol extract exhibited largest zone of inhibition of...
July 2014
Fine structural observations on oocyte development, oogenesis and fertilization process of Cotugnia polycantha Diamar, 1893 (Cestoda:Davaineidae) infecting doves (Streptopelia senegalensis)
Adult Cotugnia polycantha was recovered from the intestine of infected Streptopelia senegalensis. The fine structure of the ovary, oogenesis and fertilization process are described. The oocytes undergo characteristic cytological changes, that lead to oocyte maturation. Early stages of mature oocytes showed synaptonemal complexes in the nucleus. Lipid droplets appeared in late stages of mature oocyte. Mature oocytes are...
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