African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12300

Full Length Research Paper

Micropropagation of two species of Micranthocereus (Cactaceae) with ornamental potential native to Bahia, Brazil

Laila Mandel Civatti
  • Laila Mandel Civatti
  • Programa de Pós-Graduação em Genética e Biodiversidade, Universidade Federal da Bahia, UFBA, Rua Barão de Geremoabo, 147 - Campus de Ondina, CEP: 40170-290 Salvador, Bahia, Brasil
  • Google Scholar
Maria Nazaré Guimarães Marchi
  • Maria Nazaré Guimarães Marchi
  • Instituto Federal de Educação, Ciência e Tecnologia Baiano, BR 420 (Rodovia Santa Inês – Ubaíra), CEP: 45320-000, Zona Rural, Bahia, Brasil
  • Google Scholar
Moema Cortizo Bellintani
  • Moema Cortizo Bellintani
  • Programa de Pós-Graduação em Genética e Biodiversidade, Universidade Federal da Bahia, UFBA, Rua Barão de Geremoabo, 147 - Campus de Ondina, CEP: 40170-290 Salvador, Bahia, Brasil
  • Google Scholar


  •  Received: 18 January 2017
  •  Accepted: 08 March 2017
  •  Published: 05 April 2017

Abstract

The two target cactus species in this study, Micranthocereus flaviflorus subsp. densiflorus (Buining and Brederoo) P.J.Braun and Esteves and Micranthocereus. polyanthus subsp. alvinii M. Machado and Hofacker, are endemic to the state of Bahia and have ornamental value. Therefore, this work proposed to establish M. flaviflorus in vitro, as well as to micropropagate both species. The temperature regimes 25°C and 30°C and the alternating temperatures of 15 to 25°C were tested for in vitro germination of M. flaviflorus seeds, which achieved higher germination rates at 25°C. Regarding nutrient media, the lower water potentials of MS and MS/2 media, when compared to agar, allowed the germination of M. flaviflorus seeds. Furthermore, the test of plant growth regulators for in vitro multiplication consisted of regulator concentrations in a factorial arrangement (2x4): Naphthaleneacetic acid (NAA) (0 and 1.34 μmol L-1) and Kinetin (KIN) (0, 6.74, 20.22 and 40.44 μmol L-1) in MS/2 media. Shoots of different lengths derived from in vitro propagation were removed from explants and directly planted in plastic cups for survival appraisal. In the in vitro propagation of the studied species, the association of the highest KIN concentration (40.44 μmol L-1) to 1.34 μmol L-1 of NAA significantly reduced the number of shoots per explant. The use of 1.34 μmol L-1 of NAA without cytokinin is suggested for in vitro multiplication in both species. Shoot size was determinant for survival during acclimatization, and so, the usage of shoots at least 0.6 and 1.5 cm long for M. flaviflorus and M. polyanthus, respectively, is recommended for ex vitro establishment.

 

Key words: Acclimatization, Brazilian flora, Cactaceae, conservation.