June 2020
Responses of soybeans (Glycine max (L.) Merrill) associated with variable plant density stress applied at different phenological stages: Plasticity or elasticity?
The study evaluated developmental responses associated with plant population density stress applied at different phenological phases and effects on grain yield in determinate and indeterminate soybean (Glycine max L.). A split-split plot design with four replications, with variety (main plot), plant density (sub plot) and thinning time (sub-subplot) was adopted. Two determinate genotypes (Lukanga and SC Semeki) and an...
June 2020
Sub-acute and chronic toxicity of silver nanoparticles synthesized by Azadirachta indica extract
In this study, biosynthesis of silver nanoparticles (AgNPs) and its toxicity were investigated. Different functional groups responsible for adsorption, morphology and absorption of the nanoparticle were characterized using UV-Vis, Fourier transmission infrared spectroscopy (FTIR), and scanning electron microscope (SEM) analyses, respectively. The toxicity of orally administered Azadirachta indica AgNPs was assessed in...
June 2020
Optimization of plant growth regulators for meristem initiation and subsequent multiplication of five virus tested elite sweet potato varieties from Ethiopia
Vegetatively propagated crops are exposed to pests every growing season, providing them with the opportunity to feed on the plants and transmit diseases. Meristem culture is an important method to clean for viruses to restore high yields. Media optimizations with different concentrations and combinations of 6-benzylamino purine (BAP) and Naphthalene Acetic Acid (NAA) were tested for their effects on shoot initiation...
June 2020
Selection for and biochemical characterization of DDT resistance in laboratory strains of Anopheles arabiensis
Resistance to conventional insecticides still constitutes a major obstacle to control of malaria vectors. Xenobiotic pollutants encountered by aquatic stages of natural populations of malarial vector species in agricultural and domestic environment are often selected due to resistance to various insecticides. The Laboratory Matatuine (MAT) and Kayamba (KGB) strains of Anopheles arabiensis were subjected to controlled...
June 2020
Comparison and optimization for DNA extraction of okra (Abelmoschus esculentus L. Moench)
The extraction of high-quality DNA from okra (Abelmoschus esculentus L. Moench) is notoriously troublesome due to the high contents of polysaccharides, polyphenols, and different secondary metabolites. We have tested seven extraction buffers on silica dried okra leaves. Here, we describe a simple, rapid and modified procedure for high-quality DNA extraction from okra, which is amenable for downstream analyses. In...
June 2020
Lactic acid bacteria fermentation of coconut milk and its effect on the nutritional, phytochemical, antibacterial and sensory properties of virgin coconut oil produced
Coconut oil has profound health benefits but the high content of fatty acids is a concern to many consumers, as several processing methods have failed to produce oil with considerable change in fatty acid content. In this study, selected lactic acid bacteria including Lactobacillus plantarum, Lactobacillus pentosus, Leuconostoc mesenteroides and Enterococcus faecium were used to ferment coconut milk for production of...
June 2020
TetraSOD® activates the antioxidant response pathway in human cells: An in vitro approach
The combined action of both endogenous and exogenous factors stimulates the production of free radicals and reactive oxygen species (ROS) in cells. They are neutralized by an elaborate antioxidant defense system, superoxide dismutase (SOD) being the first line of enzymes involved in ROS scavenging. However, when ROS production cannot be counteracted by cellular antioxidant mechanisms oxidative stress happens, inducing...
June 2020
Potential applications of enzymes on the extraction of vitexin from dried Mas Cotek leaves
Mas Cotek or Ficus deltoidea var. deltoidea leaves extracts contain various beneficial properties such as antidiabetic, antihypoglycemic and antioxidant activities, which can be enzymatically extracted in water. Vitexin is one of the antioxidants and remarkable compound of flavone C-glycosides found in Mas Cotek leaves. Cellulase is one of the essential enzymes applied to hydrolyze cellulose into fermentable sugars and...
June 2020
Assessment of genetic diversity among cassava landraces using single nucleotide polymorphic markers
This study seeks to determine the genetic diversity among cassava landraces using single nucleotide polymorphic (SNP) markers. One hundred and five cassava landraces were assayed with 195 SNP markers. Major allele frequency varied from 0.500 to 0.942 with an average of 0.728. Average gene diversity, heterozygosity and polymorphic information content (PIC) were 0.359, 0.314 and 0.286 respectively. These values were...
June 2020
Cytotoxic effects of some essential oils on Mcf-7, Hfs and Hct116 cell lines
This study investigates the chemical composition and cytotoxic effects of three essential oils of rosemary, lemon and orange, as well as its effects on breast cancer (MCF-7), human foreskin fibroblasts (HFS) and colorectal carcinoma (HCT116) using gas chromatography-mass spectrometry (GC-MS) methods, leading to identification of a different compound by GC-MS. Eicosapentaenoic acid (13.79%) and heptatriacotanol (13.79%)...
Advertisement
Advertisement
