This study investigates the chemical composition and cytotoxic effects of three essential oils of rosemary, lemon and orange, as well as its effects on breast cancer (MCF-7), human foreskin fibroblasts (HFS) and colorectal carcinoma (HCT116) using gas chromatography-mass spectrometry (GC-MS) methods, leading to identification of a different compound by GC-MS. Eicosapentaenoic acid (13.79%) and heptatriacotanol (13.79%) were the major constituents present in the orange oil. Cytotoxic activity was evaluated through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) methods. Results revealed that essential oils significantly reduced the viability of MCF-7, HFS and HCT116 cells in a concentration-dependent manner. The evaluated essential oils could prove to be promising for future applications in the treatment of cancer-related diseases.
Key words: Anticancer activity, chemical composition, essential oil, cytotoxic effects, cell lines, Mcf-7, Hfs, Hct116, gas chromatography-mass spectrometry (GC–MS), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
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