African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12300

Full Length Research Paper

Cytotoxic effects of some essential oils on Mcf-7, Hfs and Hct116 cell lines

Nehad A. Shaer
  • Nehad A. Shaer
  • Chemistry Department, University College of Al Leith- Umm Al-Qura University, Makkah, Saudi Arabia.
  • Google Scholar
Nouf S. Al-Abbas
  • Nouf S. Al-Abbas
  • Chemistry Department, University College of Al Leith- Umm Al-Qura University, Makkah, Saudi Arabia.
  • Google Scholar
Amal A. Mohamed
  • Amal A. Mohamed
  • Chemistry Department, University College of Al Leith- Umm Al-Qura University, Makkah, Saudi Arabia.
  • Google Scholar
Mehal A. Alqriqri
  • Mehal A. Alqriqri
  • Stem Cell Unit, King Fahad Medical Research Center, King Abdulaziz University, Jeddah 23839, Saudi Arabia.
  • Google Scholar


  •  Received: 18 May 2020
  •  Accepted: 04 June 2020
  •  Published: 30 June 2020

Abstract

This study investigates the chemical composition and cytotoxic effects of three essential oils of rosemary, lemon and orange, as well as its effects on breast cancer (MCF-7), human foreskin fibroblasts (HFS) and colorectal carcinoma (HCT116) using gas chromatography-mass spectrometry (GC-MS) methods, leading to identification of a different compound by GC-MS. Eicosapentaenoic acid (13.79%) and heptatriacotanol (13.79%) were the major constituents present in the orange oil. Cytotoxic activity was evaluated through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) methods. Results revealed that essential oils significantly reduced the viability of MCF-7, HFS and HCT116 cells in a concentration-dependent manner. The evaluated essential oils could prove to be promising for future applications in the treatment of cancer-related diseases.

 

Key words: Anticancer activity, chemical composition, essential oil, cytotoxic effects, cell lines,  Mcf-7, Hfs, Hct116, gas chromatography-mass spectrometry (GC–MS), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.