Full Length Research Paper
Abstract
Thermostable alkaline tolerant lipase producing Bacillus licheniformis isolated from marine environment. Peanut oil cake was used as substrate for enzyme production and purified to 3.6 fold with a specific activity of 148.4 U/mg of protein with molecular weight of 35 kDa. The enzyme was 100% stable at 60°C, it retains more than 80% activity after 1 h incubation at 70°C and pH 9-11.5. Metals like Ca2+, Ba2+ and Mg2+ enhanced whereas Ni2+ and Fe3+ inhibited the enzyme activity. No effect on enzyme activity found when treated with Tween 80 and sodium deoxycholate, but completely inhibited with SDS.
Key words: Bacillus licheniformis, lipase, peanut oil cake, enzyme activity, thermostable, metals.
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